FlyBase Allele Report: Dmel\ssh[UAS.Tag:HA]

General Information

Symbol

Dmel\ssh^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0129028

Feature type

allele

Associated gene

Dmel\ssh

Associated Insertion(s)

Carried in Construct

P{UAS-ssh.N}

Key Links

Transgenic product class

wild_type

(Niwa et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Niwa et al., 2002)

Mutagen

in vitro construct

(Niwa et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-ssh.N}

Cytology

Description

UASt sequences drive expression of ssh tagged with Tag:HA.

(Niwa et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ssh

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Ojelade et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant | adult stage, with Scer\GAL4^Tub.PU

(Ojelade et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Conditional expression of ssh^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^tub.PU (GAL80ts has been used to limit expression to the adult stage) results in increased resistance to ethanol-induced sedation in adult flies compared to controls.

(Ojelade et al., 2015)

When ssh^{Scer\UAS.T:Ivir\HA1} is driven by Scer\GAL4^da.G32, no effect on hair or bristle morphogenesis is seen.

(Niwa et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ssh^UAS.Tag:HA/Scer\GAL4^NP7175 is a suppressor | partially of abnormal neuroanatomy phenotype of sick^A384/sick^Δ

(Abe et al., 2014)

NOT Suppressor of

Statement

Reference

ssh^UAS.Tag:HA, sick^A384, sick^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

ssh^UAS.Tag:HA/Scer\GAL4^NP7175 is a suppressor | partially of adult mushroom body alpha-lobe phenotype of sick^A384/sick^Δ

(Abe et al., 2014)

ssh^UAS.Tag:HA/Scer\GAL4^NP7175 is a suppressor | partially of adult mushroom body beta-lobe phenotype of sick^A384/sick^Δ

(Abe et al., 2014)

NOT Suppressor of

Statement

Reference

ssh^UAS.Tag:HA, sick^A384, sick^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of adult mushroom body alpha-lobe phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

ssh^UAS.Tag:HA, sick^A384, sick^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of adult mushroom body beta-lobe phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ssh^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^NP7175 partially suppresses the axon growth phenotype seen in the α/β mushroom body lobes of sick^A384/sick^Δ mutants.

Expression of ssh^{Scer\UAS.T:Ivir\HA1} suppresses the axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^ey-OK107. This suppression does not occur in a sick^Δ/sick^A384 mutant background.

(Abe et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

ssh^UAS.Tag:HA/Scer\GAL4^da.G32 rescues ssh^26-1/ssh⁰¹²⁰⁷

(Niwa et al., 2002)

ssh^UAS.Tag:HA/Scer\GAL4^da.G32 rescues ssh⁰¹²⁰⁷/ssh^2-4

(Niwa et al., 2002)

ssh^UAS.Tag:HA/Scer\GAL4^unspecified rescues ssh^1-11

(Niwa et al., 2002)

Partially rescues

ssh^UAS.Tag:HA/Scer\GAL4^ey-OK107 partially rescues ssh^1-11

(Ng and Luo, 2004)

Comments

Expression of ssh^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^OK107 largely rescues the axon growth and guidance defects seen in homozygous ssh^1-11 adult mushroom body clones (which are generated as neuroblast clones in newly hatched larvae). The axon growth defects seen in neuroblast clones of optic lobe and antennal lobe contralateral projection neurons are rescued by expression of ssh^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^OK107.

(Ng and Luo, 2004)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9112

y¹ w^*; P{UAS-ssh.N}30

9113

y¹ w^*; P{UAS-ssh.N}45

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S2 cells to study its phosphorylation.

(Niwa et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ssh^{Scer\UAS.T:Ivir\HA1}

ssh^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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