FlyBase Allele Report: Dmel\tsr[S3A.UAS]

General Information

Symbol

Dmel\tsr^S3A.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0177370

Feature type

allele

Associated gene

Dmel\tsr

Associated Insertion(s)

Carried in Construct

P{UAS-tsr.S3A}

Also Known As

UAS-TsrS3A, UAS-tsr.S3A

Key Links

Transgenic product class

missense_variant

(Ng and Luo, 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Ng and Luo, 2004)

Mutagen

in vitro construct

(Ng and Luo, 2004)

Progenitor genotype

Carried in construct

P{UAS-tsr.S3A}

Cytology

Description

UASt regulatory sequences drive expression of a constitutively active form of tsr (carries the amino acid replacement S3A).

(Ng and Luo, 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

tsr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Ojelade et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with Scer\GAL4^GMR13F02

(Cervantes-Sandoval et al., 2016)

abnormal memory | adult stage, with Scer\GAL4^elav.PU

(Shuai et al., 2010)

abnormal neuroanatomy | adult stage | somatic clone, with Scer\GAL4^Act.PU

(Stephan et al., 2012)

chemical sensitive | adult stage, with Scer\GAL4^Tub.PU

(Ojelade et al., 2015)

Phenotype Manifest In

adult olfactory receptor neuron Or59c | somatic clone, with Scer\GAL4^Act.PU

(Stephan et al., 2012)

Detailed Description

Statement

Reference

Expression of tsr^S3A.Scer\UAS under the control of Scer\GAL4^GMR13F02 (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) results in enhanced memory after conditioning in an olfactory conditioning assay.

(Cervantes-Sandoval et al., 2016)

Conditional expression of tsr^S3A.Scer\UAS under the control of Scer\GAL4^tub.PU (GAL80ts has been used to limit expression to the adult stage) results in increased sensitivity to ethanol-induced sedation in adult flies compared to controls.

(Ojelade et al., 2015)

Expression of tsr^S3A.Scer\UAS under the control of Scer\GAL4^Act.PU in clones results in mild-mistargeting of Or59c-expressing olfactory receptor neurons in 20% of adult brains.

(Stephan et al., 2012)

Expression of tsr^S3A.Scer\UAS under the control of Scer\GAL4^elav.PU (using tub-Gal80[ts] to limit the expression to the adult stage) significantly enhances 3 hr memory of adult flies in an aversive olfactory assay compared to controls.

(Shuai et al., 2010)

Overexpression of tsr^S3A.Scer\UAS in mushroom body neurons under the control of Scer\GAL4^OK107 does not result in gross axon or cell proliferation defects.

(Ng and Luo, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR13F02, tsr^S3A.UAS has abnormal memory phenotype, non-enhanceable by scrib^KK106065/Scer\GAL4^GMR13F02

(Cervantes-Sandoval et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^NP7175/tsr^S3A.UAS is a suppressor | partially of abnormal neuroanatomy phenotype of sick^A384/sick^Δ

(Abe et al., 2014)

Scer\GAL4^Act.PU/tsr^S3A.UAS is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of psidin¹

(Stephan et al., 2012)

tsr^S3A.UAS, Scer\GAL4^ey-OK107 is a suppressor | partially of abnormal neuroanatomy phenotype of Pak^UAS.Tag:MYC, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

tsr^S3A.UAS, Scer\GAL4^ey-OK107 is a suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Scer\GAL4^ey-OK107/tsr^S3A.UAS is a suppressor of abnormal neuroanatomy | somatic clone phenotype of ssh^1-11

(Ng and Luo, 2004)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^NP7175/tsr^S3A.UAS is a suppressor | partially of adult mushroom body alpha-lobe phenotype of sick^A384/sick^Δ

(Abe et al., 2014)

Scer\GAL4^NP7175/tsr^S3A.UAS is a suppressor | partially of adult mushroom body beta-lobe phenotype of sick^A384/sick^Δ

(Abe et al., 2014)

Scer\GAL4^Act.PU/tsr^S3A.UAS is a suppressor of adult olfactory receptor neuron Or59c | somatic clone phenotype of psidin¹

(Stephan et al., 2012)

tsr^S3A.UAS, Scer\GAL4^ey-OK107 is a suppressor | partially of adult mushroom body phenotype of Pak^UAS.Tag:MYC, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Scer\GAL4^ey-OK107/tsr^S3A.UAS is a suppressor of optic lobe neuron & axon | somatic clone phenotype of ssh^1-11

(Ng and Luo, 2004)

tsr^S3A.UAS, Scer\GAL4^ey-OK107 is a suppressor of adult mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Scer\GAL4^ey-OK107/tsr^S3A.UAS is a suppressor of adult mushroom body & axon | somatic clone phenotype of ssh^1-11

(Ng and Luo, 2004)

Scer\GAL4^ey-OK107/tsr^S3A.UAS is a suppressor of antennal lobe & axon | somatic clone phenotype of ssh^1-11

(Ng and Luo, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of scrib^KK106065 does not affect the memory performance of flies expressing tsr^S3A.Scer\UAS under the control of Scer\GAL4^GMR13F02 (in combination with a Gal80[ts] transgene to restrict expression to the adult stage).

(Cervantes-Sandoval et al., 2016)

Expression of tsr^S3A.Scer\UAS under the control of Scer\GAL4^NP7175 significantly suppresses the axon growth phenotype seen in the α/β mushroom body lobes of sick^A384/sick^Δ mutants.

(Abe et al., 2014)

Expression of tsr^S3A.Scer\UAS under the control of Scer\GAL4^Act.PU reduces the axon mistargeting phenotype seen in Or59c-expressing psidin¹ olfactory receptor neuron clones by 14%.

(Stephan et al., 2012)

The axon growth and guidance defects seen in homozygous ssh^1-11 adult mushroom body clones (which are generated as neuroblast clones in newly hatched larvae) are almost completely suppressed by co-expression of tsr^S3A.Scer\UAS under the control of Scer\GAL4^OK107. The axon growth defects seen in neuroblast clones of optic lobe and antennal lobe contralateral projection neurons are partially suppressed by co-expression of tsr^S3A.Scer\UAS under the control of Scer\GAL4^OK107.

(Ng and Luo, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^ey-OK107/tsr^S3A.UAS partially rescues tsr^N96A

(Ng and Luo, 2004)

Comments

Expression of tsr^S3A.Scer\UAS under the control of Scer\GAL4^OK107 partially rescues the cell proliferation defects seen in homozygous tsr^N96A adult mushroom body clones (which are generated as neuroblast clones in newly hatched larvae); only γ and α/β neurons are generated in these clones. Expression of tsr^S3A.Scer\UAS under the control of Scer\GAL4^OK107 partially rescues the axon growth defects seen in single cell tsr^N96A γ neuron clones in the mushroom body.

(Ng and Luo, 2004)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9236

P{UAS-tsr.S3A}4.1, y¹ w^*; βTub60D^Pin-1/CyO

9237

y¹ w^*; βTub60D^Pin-1/CyO; P{UAS-tsr.S3A}4.2.3/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin

Discoverer