FlyBase Allele Report: Dmel\hiw[UAS.fl]

General Information

Symbol

Dmel\hiw^UAS.fl

Species

D. melanogaster

Name

FlyBase ID

FBal0215862

Feature type

allele

Associated gene

Dmel\hiw

Associated Insertion(s)

Carried in Construct

P{UAS-hiw.fl}

Also Known As

UAS-Hiw

Key Links

Transgenic product class

wild_type

(Wu et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Wu et al., 2005)

Mutagen

in vitro construct

(Wu et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-hiw.fl}

Cytology

Description

UASt regulatory sequences drive expression of full-length hiw coding sequences (15.7kb).

(Wu et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

hiw

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with Scer\GAL4^NP4132

(Huang et al., 2012)

abnormal memory, with Scer\GAL4^NP7175

(Huang et al., 2012)

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Shen and Ganetzky, 2009)

decreased cell growth rate, with Scer\GAL4^elav-C155

(Shen and Ganetzky, 2009)

Phenotype Manifest In

neuromuscular junction, with Scer\GAL4^elav-C155

(Shen and Ganetzky, 2009)

Detailed Description

Statement

Reference

Ectopic expression of hiw^Scer\UAS.fl under the Scer\GAL4^elav-C155 driver does not alter the number of neuromuscular junction boutons in third instar larvae compared to controls.

(Wong et al., 2015)

Expression of hiw^Scer\UAS.fl under the control of either Scer\GAL4^NP4132 or Scer\GAL4^NP7175 severely impairs long term memory formation induced by spaced training. The presence of Scer\GAL80^Mef2.mb247 eliminates this long term memory impairment. Learning is unaffected.

(Huang et al., 2012)

Expression of hiw^Scer\UAS.fl under the control of Scer\GAL4^elav.PLu has no effect on neuromuscular junction growth.

Expression of hiw^Scer\UAS.fl under the control of Scer\GAL4^elav-C155 results in mild suppression of neuromuscular junction growth.

(Shen and Ganetzky, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^elav-C155, hiw^UAS.fl has abnormal neuroanatomy phenotype, enhanceable by Atg1^Δ3D

(Shen and Ganetzky, 2009)

Scer\GAL4^elav-C155, hiw^UAS.fl has decreased cell growth rate phenotype, enhanceable by Atg1^Δ3D

(Shen and Ganetzky, 2009)

Scer\GAL4^elav-C155, hiw^UAS.fl has abnormal neuroanatomy phenotype, enhanceable by Atg2^EP3697

(Shen and Ganetzky, 2009)

Scer\GAL4^elav-C155, hiw^UAS.fl has decreased cell growth rate phenotype, enhanceable by Atg2^EP3697

(Shen and Ganetzky, 2009)

Scer\GAL4^elav-C155, hiw^UAS.fl has abnormal neuroanatomy phenotype, enhanceable by Atg6⁰⁰⁰⁹⁶

(Shen and Ganetzky, 2009)

Scer\GAL4^elav-C155, hiw^UAS.fl has decreased cell growth rate phenotype, enhanceable by Atg6⁰⁰⁰⁹⁶

(Shen and Ganetzky, 2009)

Suppressor of

Statement

Reference

hiw^UAS.fl, Scer\GAL4^elav.PLu is a suppressor of abnormal neuroanatomy phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

hiw^UAS.fl, Scer\GAL4^elav.PLu is a suppressor of increased cell growth rate phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

Other

Statement

Reference

Scer\GAL4^elav-C155, Trpml¹, hiw^UAS.fl has abnormal neuroanatomy | third instar larval stage phenotype

(Wong et al., 2015)

Scer\GAL4^elav-C155, Trpml¹, hiw^UAS.fl has lethal - all die during embryonic stage | recessive phenotype

(Wong et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^elav-C155, hiw^UAS.fl has neuromuscular junction phenotype, enhanceable by Atg1^Δ3D

(Shen and Ganetzky, 2009)

Scer\GAL4^elav-C155, hiw^UAS.fl has neuromuscular junction phenotype, enhanceable by Atg2^EP3697

(Shen and Ganetzky, 2009)

Scer\GAL4^elav-C155, hiw^UAS.fl has neuromuscular junction phenotype, enhanceable by Atg6⁰⁰⁰⁹⁶

(Shen and Ganetzky, 2009)

Suppressor of

Statement

Reference

hiw^UAS.fl, Scer\GAL4^elav.PLu is a suppressor of NMJ bouton phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

hiw^UAS.fl, Scer\GAL4^elav.PLu is a suppressor of neuromuscular junction phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

Other

Statement

Reference

Scer\GAL4^elav-C155, Trpml¹, hiw^UAS.fl has neuromuscular junction | third instar larval stage phenotype

(Wong et al., 2015)

Scer\GAL4^elav-C155, Trpml¹, hiw^UAS.fl has NMJ bouton | third instar larval stage phenotype

(Wong et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Ectopic expression of hiw^Scer\UAS.fl under the Scer\GAL4^elav-C155 driver in the Trpml¹/+ heterozygous background causes a decrease in the number of neuromuscular junction boutons in third instar larvae compared to controls.

Ectopic expression of hiw^Scer\UAS.fl under the Scer\GAL4^elav-C155 driver in the Trpml¹/Trpml¹ homozygous background causes a decrease in the number of neuromuscular junction boutons in third instar larvae compared to controls.

Supplementing food with ML-SA1 (ML-synthetic agonist) does restore normal synaptic growth (number of neuromuscular junction boutons) in third instar Trpml¹/+ heterozygous larvae expressing hiw^Scer\UAS.fl under the Scer\GAL4^elav-C155 driver.

(Wong et al., 2015)

Co-expression of hiw^Scer\UAS.fl with Atg1^Scer\UAS.cSa, under the control of Scer\GAL4^elav-C155 significantly suppresses Atg1^Scer\UAS.cSa-mediated neuromuscular junction overgrowth.

The modest neuromuscular undergrowth seen upon expression of hiw^Scer\UAS.fl under the control of Scer\GAL4^elav-C155 is enhanced in a Atg1^Δ3D, Atg2^EP3697 or Atg6⁰⁰⁰⁹⁶ heterozygous background.

(Shen and Ganetzky, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^VGlut-OK371/hiw^UAS.fl rescues hiw⁰⁵²

(Neukomm et al., 2014)

Scer\GAL4^{elav.Switch.PO}/hiw^UAS.fl rescues hiw^ND9

(Huang et al., 2012)

hiw^UAS.fl/Scer\GAL4^elav.PLu rescues hiw^ND8

(Wu et al., 2005)

Fails to rescue

hiw^UAS.fl/Scer\GAL4^elav.PLu fails to rescue hiw^ND8

(Wu et al., 2005)

Comments

Expression of hiw^Scer\UAS.fl under the control of Scer\GAL4^VGlut-OK371 suppresses the strong protective effect of hiw⁰⁵² on L1 vein neurons following axotomy. As in wild type, the severed axons undergo fragmentation.

(Neukomm et al., 2014)

Expression of hiw^Scer\UAS.fl under the control of Scer\GAL4^{elav.Switch.PO} (in the presence of RU486) rescues the aversive long term memory defects seen in hiw^ND9 mutant flies.

(Huang et al., 2012)

Presynaptic expression of hiw^Scer\UAS.fl (under the control of Scer\GAL4^elav.PLu) resuces the deficit in both spontaneous and evoked neurotransmitter release in hiw^ND8 hemizygotes. The quantal content, which is estimated by the mean mEJP amplitude, is also rescued.

Expresion of hiw^Scer\UAS.fl postsynaptically, under the control of Scer\GAL4^G7 fails to rescue the hiw^ND8 synaptic overgrowth phenotype.

(Wu et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hiw^Scer\UAS.fl

hiw^UAS.fl

Name Synonyms

Secondary FlyBase IDs

References (7)

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