FlyBase Allele Report: Dmel\hiw[ΔRING.UAS]

General Information

Symbol

Dmel\hiw^ΔRING.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0215866

Feature type

allele

Associated gene

Dmel\hiw

Associated Insertion(s)

Carried in Construct

P{UAS-hiw.ΔRING}

Also Known As

UAS-HiwΔRING

Key Links

Transgenic product class

missense_variant

(Wu et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Wu et al., 2005)

Mutagen

in vitro construct

(Wu et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-hiw.ΔRING}

Cytology

Description

Amino acid replacement: C4991S.

(Wu et al., 2005)

UAS regulatory sequences drive expression of hiw coding sequence in which the first two cysteine residues (C4991 and C4994) in the RING domain are mutagenised to serine.

(Wu et al., 2005)

Amino acid replacement: C4994S.

(Wu et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

hiw

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with Scer\GAL4^c739

(Huang et al., 2012)

abnormal memory, with Scer\GAL4^{elav.Switch.PO}

(Huang et al., 2012)

abnormal memory, with Scer\GAL4^NP4132

(Huang et al., 2012)

abnormal memory, with Scer\GAL4^NP6024

(Huang et al., 2012)

abnormal memory, with Scer\GAL4^NP7175

(Huang et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of hiw^{ΔRING.Scer\UAS} under the control of Scer\GAL4^{elav.Switch.PO} display significantly enhanced aversive long term memory.

Expression of hiw^{ΔRING.Scer\UAS} under the control of Scer\GAL4^NP4132 leads to enhanced aversive long term memory after one-session training. Similar enhancement is seen when hiw^{ΔRING.Scer\UAS} is expressed under the control of any of Scer\GAL4^NP7175, Scer\GAL4^NP6024 or Scer\GAL4^c739. No enhancement is seen using the Scer\GAL4^NP5286 or Scer\GAL4^repo drivers. Learning is unaffected.

Sensorimotor abilities are impaired when hiw^{ΔRING.Scer\UAS} is expressed under the control of either Scer\GAL4^c739 or Scer\GAL4^NP7175 when compared to hiw^{ΔRING.Scer\UAS}-only controls, but is not significantly different from the driver only controls. Sensorimotor ability is unaffected when hiw^{ΔRING.Scer\UAS} is expressed under the control of either Scer\GAL4^NP4132 or Scer\GAL4^NP6024.

The morphological structure of the mushroom body is not obviously affected when hiw^{ΔRING.Scer\UAS} is expressed under the control of Scer\GAL4^NP4132, Scer\GAL4^NP7175 or Scer\GAL4^NP6024.

The enhancement of aversive long-term memory seen in flies expressing hiw^{ΔRING.Scer\UAS} under the control of Scer\GAL4^NP4132 is eliminated when the flies are fed the protein synthesis inhibitor cycloheximide.

Expression of hiw^{ΔRING.Scer\UAS} under the control of either Scer\GAL4^NP4132 or Scer\GAL4^NP7175 (limited to 4 days using Scer\GAL80^ts.αTub84B) results in enhanced aversive long term memory formation compared with controls. Learning is unaffected.

(Huang et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^NP4132, hiw^ΔRING.UAS has abnormal memory phenotype, suppressible by Scer\GAL4^NP4132/wnd^GD13786

(Huang et al., 2012)

Scer\GAL4^NP4132, hiw^ΔRING.UAS has abnormal memory phenotype, suppressible by bsk^DN.UAS/Scer\GAL4^NP4132

(Huang et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of wnd^GD13786 suppresses the enhancement of aversive long term memory seen when hiw^{ΔRING.Scer\UAS} is expressed under the control of Scer\GAL4^NP4132. Learning is unaffected.

Expression of bsk^DN.Scer\UAS suppresses the enhancement of aversive long term memory seen when hiw^{ΔRING.Scer\UAS} is expressed under the control of Scer\GAL4^NP4132. Learning is unaffected.

(Huang et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^{elav.Switch.PO}/hiw^ΔRING.UAS fails to rescue hiw^ND9

(Huang et al., 2012)

hiw^ΔRING.UAS/Scer\GAL4^elav.PLu fails to rescue hiw^ND8

(Wu et al., 2005)

Comments

Expression of hiw^{ΔRING.Scer\UAS} under the control of Scer\GAL4^{elav.Switch.PO} (in the presence of RU486) does not rescue or further enhance the aversive long term memory defects seen in hiw^ND9 mutant flies.

(Huang et al., 2012)

Expression of hiw^{ΔRING.Scer\UAS} under the control of Scer\GAL4^elav.PLu fails to rescue any of the synaptic morphology phenotypes of hiw^ND8.

(Wu et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hiw^{ΔRING.Scer\UAS}

hiw^ΔRING.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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