FlyBase Allele Report: Dmel\Rbf[120a]

General Information

Symbol

Dmel\Rbf^120a

Species

D. melanogaster

Name

FlyBase ID

FBal0095621

Feature type

allele

Associated gene

Dmel\Rbf

Associated Insertion(s)

P{CaSpeR}Rbf^120a

Carried in Construct

Also Known As

rbf1^120a

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Du and Dyson, 1999)

Progenitor genotype

P{CaSpeR}cx31A.2a

(Du and Dyson, 1999)

Associated Insertion(s)

P{CaSpeR}Rbf^120a

Cytology

Description

Insertion of a P{CaSpeR} element in the 5' untranslated region, approximately 700bp from the start codon.

(Du and Dyson, 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg chorion (with Rbf¹¹)

(Brumby et al., 2002)

eye (with Rbf¹¹)

(Brumby et al., 2002)

eye disc

(Truscott et al., 2014, Moon et al., 2008)

eye disc (with Rbf¹¹)

(Brumby et al., 2002)

eye disc | somatic clone

(Moon et al., 2008)

eye disc anterior to the morphogenetic furrow | somatic clone

(Hsieh et al., 2010)

eye disc morphogenetic furrow | somatic clone

(Hsieh et al., 2010)

follicle cell (with Rbf¹⁴)

(Cayirlioglu et al., 2003, Bosco et al., 2001, Cayirlioglu et al., 2001)

follicle cell & cell cycle (with Rbf¹¹)

(Brumby et al., 2002)

mitotic cell cycle (with Rbf¹⁴)

(Bosco et al., 2001)

S phase | ectopic & eye disc | posterior (with Rbf¹¹)

(Brumby et al., 2002)

Detailed Description

Statement

Reference

Rbf^120a mutant larvae are more sensitive to fasting than controls.

(Zhang et al., 2014)

Rbf^120a flies have almost normal eyes.

(Popova et al., 2011)

On average, 3.7 ectopic S-phase cells/1000 pixels are present in Rbf^120a mutant eye disc clones.

Rbf^120a mutant eye disc clones exhibit apoptosis just anterior to the morphogenetic furrow, and this is not observed in the wild-type disc.

(Hsieh et al., 2010)

Compared with wild-type controls, homozygous Rbf^120a mutant cells in the eye disc show heightened sensitivity to apoptosis after treatment with ionising irradiation.

DNA-damage induces apoptosis in Rbf^120a mutant eye discs.

(Moon et al., 2008)

In Rbf^120a/Rbf¹⁴ animals, FACS profiles of DNA content in ovarian follicle cells show a population with a ploidy of 32n not seen in wild-type.

(Shcherbata et al., 2004)

Rbf^120a/Rbf¹⁴ follicle cells show increased ploidy compared to control cells by FACS analysis.

(Cayirlioglu et al., 2003)

Rbf¹¹/Rbf^120a eye discs have a severe disruption in the post-morphogenetic furrow S phase band, but have excessive S phases in the posterior region. Despite this disruption, the adult eye phenotype is only mildly disorganised.

(Brumby et al., 2002)

Rbf^120a/Rbf¹⁴ egg chambers have a mosaic of follicle cells with DNA replication defects. Overamplification of DNA is also seen in the follicle cells. The follicle cells also have cell-cycle defects; the follicle cells undergo at least one extra S phase. DNA replication also persists in later stages of mutant follicle cells than normal.

(Bosco et al., 2001)

Some follicle cells from Rbf¹⁴/Rbf^120a ovaries undergo an additional endocycle.

(Cayirlioglu et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rbf^120a has increased cell death phenotype, enhanceable by mir-998^exc222

(Truscott et al., 2014)

Rbf^120a has increased cell death phenotype, enhanceable by Cbl^L.UAS/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

Rbf^120a has increased cell death phenotype, enhanceable by Scer\GAL4^{FRT.Rnor\Cd2.Act5C}/Cbl^S.UAS

(Truscott et al., 2014)

Rbf^120a has abnormal starvation stress response phenotype, enhanceable by Axn¹²⁷

(Zhang et al., 2014)

Rbf^120a has increased cell death | somatic clone phenotype, enhanceable by Tsc1^R453X

(Hsieh et al., 2010)

Rbf^120a has increased occurrence of cell division | larval stage | somatic clone phenotype, enhanceable by Tsc1^R453X

(Hsieh et al., 2010)

Rbf^120a has increased cell death | somatic clone phenotype, enhanceable by Tsc1^f01910

(Hsieh et al., 2010)

NOT Enhanced by

Statement

Reference

Rbf^120a has increased cell death phenotype, non-enhanceable by p53^5A-1-4

(Moon et al., 2008)

Suppressed by

Statement

Reference

Rbf^120a, Scer\GAL4^GMR.PFa, yki^{S111A.S168A.S250A.UAS.Tag:V5} has abnormal cell number | larval stage phenotype, suppressible | partially by hth^KK108831, Scer\GAL4^GMR.PFa

(Rader et al., 2023)

Rbf^120a, Scer\GAL4^GMR.PFa, yki^{S111A.S168A.S250A.UAS.Tag:V5} has increased occurrence of cell division | larval stage phenotype, suppressible | partially by hth^KK108831, Scer\GAL4^GMR.PFa

(Rader et al., 2023)

Cbl^L.UAS, Rbf^120a, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} has increased cell death phenotype, suppressible | partially by mir-998^UAS.cTa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

Cbl^S.UAS, Rbf^120a, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} has increased cell death phenotype, suppressible | partially by mir-998^UAS.cTa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

Rbf^120a has increased cell death phenotype, suppressible by mir-998^UAS.cTa/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

Rbf^120a has increased cell death phenotype, suppressible by Cbl^F165

(Truscott et al., 2014)

Rbf^120a, Tsc1^R453X has increased cell death | somatic clone phenotype, suppressible by Rheb^2D1

(Hsieh et al., 2010)

Rbf^120a, Tsc1^f01910 has increased cell death | somatic clone phenotype, suppressible by E2f1^rM729

(Hsieh et al., 2010)

Rbf^120a, gig¹⁹² has increased cell death | somatic clone phenotype, suppressible by S6k^l-1

(Hsieh et al., 2010)

Rbf^120a has increased cell death | somatic clone phenotype, suppressible by Rheb^2D1

(Hsieh et al., 2010)

Rbf^120a has increased cell death | somatic clone phenotype, suppressible by S6k^l-1

(Hsieh et al., 2010)

Rbf^120a has increased cell death phenotype, suppressible by Dp^unspecified

(Moon et al., 2008)

Rbf^120a has increased cell death phenotype, suppressible by p53^5A-1-4

(Moon et al., 2008)

Rbf^120a has increased cell death | recessive phenotype, suppressible by rpr⁸⁷

(Moon et al., 2008)

NOT suppressed by

Statement

Reference

Rbf^120a has increased cell death phenotype, non-suppressible by mir-11^UAS.DsRed2/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

Rbf^120a has increased cell death phenotype, non-suppressible by p53^5A-1-4

(Moon et al., 2008)

Enhancer of

Statement

Reference

Rbf^120a/Rbf[+], Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Scer\GAL4^GMR.PF, lin-52^GD12885

(Bhaskar et al., 2014)

Rbf^120a is an enhancer of abnormal starvation stress response phenotype of Axn¹²⁷

(Zhang et al., 2014)

Rbf^120a is an enhancer of increased cell death | somatic clone phenotype of Tsc1^R453X

(Hsieh et al., 2010)

Rbf^120a is an enhancer of increased cell death | somatic clone phenotype of Tsc1^f01910

(Hsieh et al., 2010)

Rbf^120a/E2f1^rM729 is an enhancer of increased cell death | somatic clone phenotype of Tsc1^f01910

(Hsieh et al., 2010)

Rbf^120a is an enhancer of increased cell death | somatic clone phenotype of gig¹⁹²

(Hsieh et al., 2010)

Rbf^120a is an enhancer of visible phenotype of Dp^GMR.PD, E2f1^GMR.PD

(Du and Dyson, 1999)

NOT Enhancer of

Statement

Reference

Rbf^120a is a non-enhancer of decreased cell death | recessive phenotype of hid⁰⁵⁰¹⁴

(Moon et al., 2008)

Suppressor of

Statement

Reference

Rbf¹⁴/Rbf^120a is a suppressor of lethal phenotype of E2f1⁹¹/E2f1^rM729

(Du, 2000)

Rbf^120a/Rbf[+] is a suppressor of visible phenotype of Hsap\CDKN1A^GMR.PH

(Du and Dyson, 1999)

NOT Suppressor of

Statement

Reference

Rbf^120a is a non-suppressor of decreased cell death | recessive phenotype of hid⁰⁵⁰¹⁴

(Moon et al., 2008)

Other

Statement

Reference

Rbf^120a, Scer\GAL4^GMR.PFa, yki^{S111A.S168A.S250A.UAS.Tag:V5} has increased occurrence of cell division | larval stage phenotype

(Rader et al., 2023)

Rbf^120a, Scer\GAL4^GMR.PFa, yki^{S111A.S168A.S250A.UAS.Tag:V5} has abnormal cell number | larval stage phenotype

(Rader et al., 2023)

Rbf^120a, Scer\GAL4^Act5C.PU, emc^d09015 has visible | somatic clone phenotype

(Popova et al., 2011)

Rbf^120a, Scer\GAL4^Act5C.PU, emc^UAS.cdCa has visible | somatic clone phenotype

(Popova et al., 2011)

Df(3R)16C3a, P{CG4287-CG5516-(Rbf2).S}, P{mor⁺11.8}, Rbf^120a has visible phenotype

(Stevaux et al., 2005)

E2f1¹⁶⁴/E2f1⁹¹, Rbf¹⁴/Rbf^120a has abnormal size | larval stage phenotype

(Du, 2000)

E2f1¹⁶⁴/E2f1⁹¹, Rbf¹⁴/Rbf^120a has abnormal body size | larval stage phenotype

(Du, 2000)

E2f1¹⁶⁴/E2f1⁹¹, Rbf¹⁴/Rbf^120a has increased occurrence of cell division | larval stage phenotype

(Du, 2000)

E2f1¹⁶⁴/E2f1⁹¹, Rbf¹⁴/Rbf^120a has abnormal developmental rate | larval stage phenotype

(Du, 2000)

E2f1¹⁶⁴/E2f1⁹¹, Rbf¹⁴/Rbf^120a has increased cell death | larval stage phenotype

(Du, 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rbf^120a has eye disc phenotype, enhanceable by mir-998^exc222

(Truscott et al., 2014)

Rbf^120a has eye disc phenotype, enhanceable by Cbl^L.UAS/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

Rbf^120a has eye disc phenotype, enhanceable by Scer\GAL4^{FRT.Rnor\Cd2.Act5C}/Cbl^S.UAS

(Truscott et al., 2014)

Rbf^120a has eye disc anterior to the morphogenetic furrow | somatic clone phenotype, enhanceable by Tsc1^R453X

(Hsieh et al., 2010)

Rbf^120a has eye disc morphogenetic furrow | somatic clone phenotype, enhanceable by Tsc1^R453X

(Hsieh et al., 2010)

Rbf^120a has eye disc anterior to the morphogenetic furrow | somatic clone phenotype, enhanceable by Tsc1^f01910

(Hsieh et al., 2010)

Rbf^120a has eye disc morphogenetic furrow | somatic clone phenotype, enhanceable by Tsc1^f01910

(Hsieh et al., 2010)

NOT Enhanced by

Statement

Reference

Rbf^120a has eye disc phenotype, non-enhanceable by p53^5A-1-4

(Moon et al., 2008)

Rbf^120a/Rbf¹¹ has S phase | ectopic & eye disc | posterior phenotype, non-enhanceable by brm^25S14/mor^35S1

(Brumby et al., 2002)

Rbf^120a/Rbf¹¹ has eye disc phenotype, non-enhanceable by brm^25S14/mor^35S1

(Brumby et al., 2002)

Rbf^120a/Rbf¹¹ has eye phenotype, non-enhanceable by brm^25S14/mor^35S1

(Brumby et al., 2002)

Suppressed by

Statement

Reference

Rbf^120a, Scer\GAL4^GMR.PFa, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye disc | larval stage phenotype, suppressible | partially by hth^KK108831, Scer\GAL4^GMR.PFa

(Rader et al., 2023)

Rbf^120a, Scer\GAL4^GMR.PFa, yki^{S111A.S168A.S250A.UAS.Tag:V5} has photoreceptor cell R8 | larval stage phenotype, suppressible | partially by hth^KK108831, Scer\GAL4^GMR.PFa

(Rader et al., 2023)

Cbl^L.UAS, Rbf^120a, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} has eye disc phenotype, suppressible | partially by mir-998^UAS.cTa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

Cbl^S.UAS, Rbf^120a, Scer\GAL4^{FRT.Rnor\Cd2.Act5C} has eye disc phenotype, suppressible | partially by mir-998^UAS.cTa, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

Rbf^120a has eye disc phenotype, suppressible by mir-998^UAS.cTa/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

Rbf^120a has eye disc phenotype, suppressible by Cbl^F165

(Truscott et al., 2014)

Rbf^120a, Tsc1^R453X has eye disc morphogenetic furrow | somatic clone phenotype, suppressible by Rheb^2D1

(Hsieh et al., 2010)

Rbf^120a, Tsc1^f01910 has eye disc | somatic clone phenotype, suppressible by E2f1^rM729

(Hsieh et al., 2010)

Rbf^120a, Tsc1^f01910 has eye disc morphogenetic furrow | somatic clone phenotype, suppressible by E2f1^rM729

(Hsieh et al., 2010)

Rbf^120a, gig¹⁹² has eye disc | somatic clone phenotype, suppressible by S6k^l-1

(Hsieh et al., 2010)

Rbf^120a has eye disc morphogenetic furrow | somatic clone phenotype, suppressible by Rheb^2D1

(Hsieh et al., 2010)

Rbf^120a has eye disc anterior to the morphogenetic furrow | somatic clone phenotype, suppressible by Rheb^2D1

(Hsieh et al., 2010)

Rbf^120a has eye disc morphogenetic furrow | somatic clone phenotype, suppressible by S6k^l-1

(Hsieh et al., 2010)

Rbf^120a has eye disc anterior to the morphogenetic furrow | somatic clone phenotype, suppressible by S6k^l-1

(Hsieh et al., 2010)

Rbf^120a, Tsc1^R453X has eye disc | somatic clone phenotype, suppressible by Rheb^2D1

(Hsieh et al., 2010)

Rbf^120a has eye disc phenotype, suppressible by Dp^unspecified

(Moon et al., 2008)

Rbf^120a has eye disc phenotype, suppressible by p53^5A-1-4

(Moon et al., 2008)

Rbf^120a has eye disc phenotype, suppressible by rpr⁸⁷

(Moon et al., 2008)

NOT suppressed by

Statement

Reference

Rbf^120a has eye disc phenotype, non-suppressible by mir-11^UAS.DsRed2/Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Truscott et al., 2014)

Rbf^120a has eye disc phenotype, non-suppressible by p53^5A-1-4

(Moon et al., 2008)

Rbf^120a/Rbf¹¹ has eye disc phenotype, non-suppressible by brm^25S14/mor^35S1

(Brumby et al., 2002)

Rbf^120a/Rbf¹¹ has eye phenotype, non-suppressible by brm^25S14/mor^35S1

(Brumby et al., 2002)

Rbf^120a/Rbf¹¹ has S phase | ectopic & eye disc | posterior phenotype, non-suppressible by brm^25S14/mor^35S1

(Brumby et al., 2002)

Enhancer of

Statement

Reference

Rbf^120a/Rbf[+], Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, lin-52^GD12885

(Bhaskar et al., 2014)

Rbf^120a/Rbf[+], Scer\GAL4^GMR.PF is an enhancer of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, lin-52^GD12885

(Bhaskar et al., 2014)

Rbf^120a/Rbf[+], Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, lin-52^GD12885

(Bhaskar et al., 2014)

Rbf^120a is an enhancer of eye disc morphogenetic furrow | somatic clone phenotype of Tsc1^R453X

(Hsieh et al., 2010)

Rbf^120a is an enhancer of eye disc morphogenetic furrow | somatic clone phenotype of Tsc1^f01910

(Hsieh et al., 2010)

Rbf^120a is an enhancer of eye disc | somatic clone phenotype of Tsc1^f01910

(Hsieh et al., 2010)

Rbf^120a/E2f1^rM729 is an enhancer of eye disc | somatic clone phenotype of Tsc1^f01910

(Hsieh et al., 2010)

Rbf^120a is an enhancer of eye disc | somatic clone phenotype of gig¹⁹²

(Hsieh et al., 2010)

Rbf^120a is an enhancer of eye disc | somatic clone phenotype of Tsc1^R453X

(Hsieh et al., 2010)

Rbf^120a is an enhancer of eye phenotype of Dp^GMR.PD, E2f1^GMR.PD

(Du and Dyson, 1999)

NOT Enhancer of

Statement

Reference

Rbf^120a is a non-enhancer of eye disc phenotype of hid⁰⁵⁰¹⁴

(Moon et al., 2008)

Suppressor of

Statement

Reference

Rbf¹⁴/Rbf^120a is a suppressor of embryonic/larval optic lobe phenotype of E2f1⁹¹/E2f1^rM729

(Du, 2000)

Rbf¹⁴/Rbf^120a is a suppressor of eye disc phenotype of E2f1⁹¹/E2f1^rM729

(Du, 2000)

Rbf¹⁴/Rbf^120a is a suppressor of eye disc & mitotic cell cycle phenotype of E2f1⁹¹/E2f1^rM729

(Du, 2000)

Rbf^120a/Rbf[+] is a suppressor of eye phenotype of Hsap\CDKN1A^GMR.PH

(Du and Dyson, 1999)

NOT Suppressor of

Statement

Reference

Rbf^120a is a non-suppressor of eye disc phenotype of hid⁰⁵⁰¹⁴

(Moon et al., 2008)

Other

Statement

Reference

Rbf^120a, Scer\GAL4^GMR.PFa, yki^{S111A.S168A.S250A.UAS.Tag:V5} has eye disc | larval stage phenotype

(Rader et al., 2023)

Rbf^120a, Scer\GAL4^GMR.PFa, yki^{S111A.S168A.S250A.UAS.Tag:V5} has photoreceptor cell R8 | larval stage phenotype

(Rader et al., 2023)

Rbf^120a, Scer\GAL4^Act5C.PU, emc^d09015 has eye | somatic clone phenotype

(Popova et al., 2011)

Rbf^120a, Scer\GAL4^Act5C.PU, emc^UAS.cdCa has eye | somatic clone phenotype

(Popova et al., 2011)

Rbf^120a, Scer\GAL4^Act5C.PU, emc^UAS.cdCa has cone cell | somatic clone | pupal stage phenotype

(Popova et al., 2011)

Rbf^120a, Scer\GAL4^Act5C.PU, emc^UAS.cdCa has eye photoreceptor cell | somatic clone | pupal stage phenotype

(Popova et al., 2011)

Df(3R)16C3a, P{CG4287-CG5516-(Rbf2).S}, P{mor⁺11.8}, Rbf^120a has eye phenotype

(Stevaux et al., 2005)

E2f1¹⁶⁴/E2f1⁹¹, Rbf¹⁴/Rbf^120a has eye disc morphogenetic furrow | larval stage phenotype

(Du, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

A Rbf^120a mutant background dominantly enhances the Scer\GAL4^GMR.PF>lin-52^GD12885 eye phenotype. This is accompanied by a corresponding increase in hid protein and elevated apoptosis in the eye-antennal disc.

(Bhaskar et al., 2014)

Generation of eye disc clones expressing mir-11^{Scer\UAS.T:Disc\RFP-DsRed2} does not suppress the cell death seen in Rbf^120a mutant eye discs.

Generation of eye disc clones expressing mir-998^Scer\UAS.cTa suppresses the cell death seen in Rbf^120a mutant eye discs.

The generation of mir-998^exc222 mutant eye disc clones enhances the amount of cell death seen in Rbf^120a mutant eye discs.

The generation of Cbl^L.Scer\UAS mutant eye disc clones enhances the amount of cell death seen in Rbf^120a mutant eye discs. Co-expression of mir-998^Scer\UAS.cTa blocks this suppression.

The generation of Cbl^S.Scer\UAS mutant eye disc clones enhances the amount of cell death seen in Rbf^120a mutant eye discs. Co-expression of mir-998^Scer\UAS.cTa blocks this suppression.

The generation of Cbl^F165 mutant eye disc clones suppresses the amount of cell death seen in Rbf^120a mutant eye discs.

(Truscott et al., 2014)

Axn¹²⁷ Rbf^120a double mutant larvae show enhanced sensitivity to fasting than either mutant alone.

(Zhang et al., 2014)

Rbf^120a flies expressing emc^d09015 or emc^{Scer\UAS.cdCa} in clones in the eye under the control of Scer\GAL4^Act5C.PU have rough eyes which are reduced in size. Pupal eye discs of these animals analysed at 42 hours after puparium formation often contain ommatidia with less than four cone cells per cluster and fewer than normal photoreceptor cells.

(Popova et al., 2011)

Rbf^120a Tsc1^R453X double mutant eye disc clones exhibit ectopic S-phase cells at the morphogenetic furrow. Approximately 12.4 ectopic S-phase cells/pixels are present in Rbf^120a Tsc1^R453X double mutant eye disc clones, showing a 3-fold increase compared to Rbf^120a single mutant clones.

Rbf^120a Tsc1^R453X double mutant eye disc clones exhibit a large increase in apoptosis at the morphogenetic furrow and the anterior region of the eye disc.

When Tsc1^f01910 E2f^rM729 double mutant clones are generated in Rbf^120a, the sizes of the Tsc1^f01910 E2f^rM729 double mutant clones are much smaller than those of Tsc1^f01910 single mutant clones.

The prevailing cell death phenotype observed in Rbf^120a Tsc1^f01910 double mutant clones at the morphogenetic furrow is no longer present in Rbf^120a E2f^rM729 Tsc1^f01910 triple mutant cells, indicating that the increased level of ectopic cell death observed in Rbf^120a Tsc1^f01910 double mutant cells is E2f-dependent.

Generating Tsc1^f01910 clones in Rbf^120a eye discs produce an increase in the level of ectopic cell death.

Rheb^2D1 mutant clones in a Rbf^120a mutant eye disc exhibit a decrease in apoptosis at the morphogenetic furrow compared to neighboring Rbf^120a cells. The ectopic cell death observed in Rbf^120a Tsc1^R453X double mutant cells is completely suppressed in Rheb^2D1 mutant clones.

The basal level of E2f-dependent cell death normally present in Rbf^120a mutant eye discs is suppressed in S6k^l-1 mutant cells.

The level of ectopic cell death is increased in gig¹⁹² mutant clones generated in Rbf^120a mutant eye discs.

The ectopic cell death observed in Rbf^120a gig¹⁹² is completely absent in Rbf^120a gig¹⁹² S6k^l-1 triple mutant cells.

(Hsieh et al., 2010)

The wave of apoptosis in Rbf^120a mutant eye discs is completely suppressed by Dp^unspecified.

The wave of apoptosis that appears in the absence of irradiation in Rbf^120a mutant eye discs is unaffected by p53^5A-1-4.

The elevated level of DNA-damage induced apoptosis in Rbf^120a mutant eye discs is suppressed by Dp^unspecified.

The elevated level of DNA-damage induced apoptosis in Rbf^120a mutant eye discs is suppressed by p53^5A-1-4.

Similarly to W⁰⁵⁰¹⁴ single mutants, DNA-damage induced apoptosis is completely abolished in Rbf^120a, W⁰⁵⁰¹⁴ double mutant eye discs.

The level of DNA-damage induced cell death seen in Rbf^120a mutant eye discs is clearly reduced in the presence of rpr⁸⁷. rpr⁸⁷ does not affect the level or pattern of cell death in Rbf^120a mutant eye discs before irradiation treatment.

(Moon et al., 2008)

Flies carrying Rbf^120a and homozygous for "Rbf2[M2]" (Df(3R)16C3a with all deleted genes except Rbf2 rescued by the addition of two transgene insertions: P{CG4287-CG5516-(Rbf2).S}a and P{mor⁺11.8}F3a) have a rough eye phenotype that is not seen in either Rbf^120a or "Rbf2[M2]" single mutants.

(Stevaux et al., 2005)

The addition of brm^25S14 and mor^35S1 to Rbf^120a/Rbf¹¹ animals has no effect on eye disc and eye phenotypes.

(Brumby et al., 2002)

The larval lethality of E2f⁹¹/E2f^rM729 transheterozygotes can be suppressed by Rbf^120a/Rbf¹⁴; the larvae develop into large late third instar larvae and many can pupate. Some Rbf^120a/Rbf¹⁴ ; E2f⁹¹/E2f^rM729 animals can develop into pharate adults with adult eyes, legs and wing structures. The development of these larvae is significantly retarded compared to wild-type larvae, with the earliest pupae observed at around day 11 after egg laying. BrdU incorporation is not seen in the optic lobe region of the brain in day 13 E2f⁹¹/E2f^rM729 larvae, in contrast to wild-type third instar larvae. The normal pattern of DNA replication in the optic lobe region is restored if the larvae are also carrying Rbf^120a/Rbf¹⁴. The eye discs of E2f⁹¹/E2f^rM729 larvae are very small and have only a few cells labelled with BrdU with no specific pattern. In Rbf^120a/Rbf¹⁴ ; E2f⁹¹/E2f^rM729 eye discs the overall pattern of DNA replication is normal. No significant cell death is seen in these eye discs. In late third instar discs there are about 25 rows of developing photoreceptor cells (as in wild-type discs) but there are much fewer cells anterior to the morphogenetic furrow than in wild type.

(Du, 2000)

Enhances the rough eye phenotype of flies expressing both E2f^GMR.PD and Dp^GMR.PD.

(Du and Dyson, 1999)

Xenogenetic Interactions

Statement

Reference

Dominantly suppresses the Hsap\CDKN1A^GMR.PH eye phenotype.

(Du and Dyson, 1999)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

81612

Rbf^120a/FM7i, P{ActGFP}JMR3

81613

Rbf^120a w¹¹¹⁸, P{hsFLP}1/FM7i, P{ActGFP}JMR3; Egfr^tsla/CyO, P{ActGFP}JMR1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Rbf1¹²⁰

(Cayirlioglu et al., 2003)

Rbf1^120a

(Hsieh et al., 2010)

Rbf¹²⁰

(Cayirlioglu et al., 2001)

Rbf^120a

(Rader et al., 2023, Ariss et al., 2018, Bhaskar et al., 2014)

Rbf¹⁵

(Zhang et al., 2014)

rbf1^120a

(Church et al., 2017, Bradley-Gill et al., 2016, Miles et al., 2014, Collins and Moon, 2013, Longworth et al., 2012, Popova et al., 2011, Moon et al., 2008, Stevaux et al., 2005)

rbf^120a

(Truscott et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (21)

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