FlyBase Allele Report: Dmel\Rheb[2D1]

General Information

Symbol

Dmel\Rheb^2D1

Species

D. melanogaster

Name

FlyBase ID

FBal0159910

Feature type

allele

Associated gene

Dmel\Rheb

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Stocker et al., 2003)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of type 2 diabetes mellitus

CEA with Rheb^7A1

(Murillo-Maldonado et al., 2011)

CEA with Rheb^44.1

(Murillo-Maldonado et al., 2011)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size | adult stage (with Rheb^7A1)

(Murillo-Maldonado et al., 2011)

decreased cell number | somatic clone

(Stocker et al., 2003)

decreased cell size | somatic clone

(Stocker et al., 2003)

viable (with Rheb^7A1)

(Murillo-Maldonado et al., 2011)

Phenotype Manifest In

eye | somatic clone

(Stocker et al., 2003)

ommatidium | somatic clone

(Stocker et al., 2003)

photoreceptor cell R1 | somatic clone

(Avet-Rochex et al., 2014)

photoreceptor cell R6 | somatic clone

(Avet-Rochex et al., 2014)

Detailed Description

Statement

Reference

Rheb^2D1/+ flies have normal wings.

(Kim and Choi, 2019)

Rheb^2D1 mutant clones cause a strong delay in the differentiation of R1/6 photoreceptor cells.

(Avet-Rochex et al., 2014)

Rheb^7A1/Rheb^2D1 mutant adult flies have a reduced body weight compared to heterozygous controls.

Lipid and carbohydrate content are not increased in Rheb^7A1/Rheb^2D1 mutant flies compared to controls.

Rheb^2D1/Rheb^44.1 mutant adult flies have a reduced body weight compared to heterozygous controls.

Lipid content is increased in Rheb^2D1/Rheb^44.1 mutant flies compared to controls. Carbohydrate content is similar to controls.

Homozygous Rheb^2D1/Rheb^44.1 mutant flies exhibit defective electroretinogram (ERG) readings.

Cholinesterase activity is significantly reduced in Rheb^2D1/Rheb^44.1 mutant flies compared to controls.

(Murillo-Maldonado et al., 2011)

Rheb^2D1 somatic clones in the wing disc are consistently smaller than their wild-type sister clones. The reduced clone size is accounted for by fewer and smaller cells (n = 10, P = 0.00038 for cell number, P = 0.00126 for cell size) When somatic clones of Rheb^2D1 homozygous cells are generated throughout the eye disc using Scer\FLP1^ey.PN with Scer\FRT, the resulting flies have small rough eyes.

(Stocker et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Rheb^2D1 is a suppressor of increased cell death | somatic clone phenotype of Rbf^120a

(Hsieh et al., 2010)

Rheb^2D1 is a suppressor of increased cell death | somatic clone phenotype of Rbf^120a, Tsc1^R453X

(Hsieh et al., 2010)

Rheb^2D1/Rheb^7A1 is a suppressor of lethal | larval stage phenotype of Tsc1^2X1/Tsc1^1A2

(Stocker et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rheb^2D1 has photoreceptor cell R1 | somatic clone phenotype, suppressible by unk^ex24

(Avet-Rochex et al., 2014)

Rheb^2D1 has photoreceptor cell R6 | somatic clone phenotype, suppressible by unk^ex24

(Avet-Rochex et al., 2014)

Rheb^2D1 has eye | somatic clone phenotype, suppressible by Pten¹¹⁷/Pten¹¹⁷

(Stocker et al., 2003)

Rheb^2D1 has ommatidium | somatic clone phenotype, suppressible by Pten¹¹⁷/Pten¹¹⁷

(Stocker et al., 2003)

NOT suppressed by

Statement

Reference

Rheb^2D1 has eye | somatic clone phenotype, non-suppressible by Tsc1^2G3/Tsc1^2G3

(Stocker et al., 2003)

Rheb^2D1 has ommatidium | somatic clone phenotype, non-suppressible by Tsc1^2G3/Tsc1^2G3

(Stocker et al., 2003)

Enhancer of

Statement

Reference

Rheb^2D1/Rheb[+] is an enhancer of wing blade phenotype of CCT4^KK108551, Scer\GAL4^ptc.PU

(Kim and Choi, 2019)

Rheb^2D1/Rheb[+] is an enhancer of wing blade phenotype of CCT4^NIG.5525R, Scer\GAL4^ptc.PU

(Kim and Choi, 2019)

Suppressor of

Statement

Reference

Rheb^2D1 is a suppressor of ommatidium | somatic clone phenotype of unk^ex24

(Avet-Rochex et al., 2014)

Rheb^2D1 is a suppressor of rhabdomere | somatic clone phenotype of unk^ex24

(Avet-Rochex et al., 2014)

Rheb^2D1 is a suppressor of eye disc | somatic clone phenotype of Rbf^120a, Tsc1^R453X

(Hsieh et al., 2010)

Rheb^2D1 is a suppressor of eye disc morphogenetic furrow | somatic clone phenotype of Rbf^120a, Tsc1^R453X

(Hsieh et al., 2010)

Rheb^2D1 is a suppressor of eye disc morphogenetic furrow | somatic clone phenotype of Rbf^120a

(Hsieh et al., 2010)

Rheb^2D1 is a suppressor of eye disc anterior to the morphogenetic furrow | somatic clone phenotype of Rbf^120a

(Hsieh et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

unk^ex24, Rheb^2D1 mutant eye clones display wild-type differentiation of the R1/6 photoreceptor cells, indicating unk suppresses the strong delay in differentiation exhibited in Rheb mutant clones. In the adult eye, the elliptical and split rhabdomere phenotype seen in unk^ex24 clones is suppressed in unk^ex24, Rheb^2D1 mutant clones. However, these double mutant clones contain mis-rotated ommatidia and missing photoreceptors.

(Avet-Rochex et al., 2014)

Rheb^2D1 mutant clones in a Rbf^120a mutant eye disc exhibit a decrease in apoptosis at the morphogenetic furrow compared to neighboring Rbf^120a cells. The ectopic cell death observed in Rbf^120a Tsc1^R453X double mutant cells is completely suppressed in Rheb^2D1 mutant clones.

(Hsieh et al., 2010)

The small rough eye phenotype see in flies with Rheb^2D1 somatic clones throughout the eye disc generated using Scer\FLP1^ey.PN and Scer\FRT, is largely suppressed by Pten¹¹⁷/Pten¹¹⁷, but is unaffected by Tsc1^2G3/Tsc1^2G3. Rheb^2D1/Rheb^7A1 suppresses the pupal lethality due to Tsc1^2X1/Tsc1^1A2. The resulting animals are smaller than doubly heterozygous controls (Tsc1^2X1 Rheb^2D1/+) at both pupal and adult stages.

(Stocker et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rheb^2D1

(Maierbrugger et al., 2020, Kim and Choi, 2019, Avet-Rochex et al., 2014, Kapuria et al., 2012, Hsieh et al., 2010, Chang and Neufeld, 2009, Kim et al., 2008, Khurana et al., 2006)

dRheb^2D1

(Murillo-Maldonado et al., 2011)

rheb^2D1

(Zitserman et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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