Imprecise excision of P{EPgy2}unkEY03956 generates a deletion in unk that begins 557bp into the second intron and ends 150bp into the fourth exon.
Imprecise excision of P{EPgy2}unkEY03956 generates a deletion in unk that begins 557bp into the second intron and ends 150bp into the fourth exon.
The induction of (near) whole-eye unkex24 clones leads to significantly larger eyes than upon the induction of (near) whole-eye control clones. unkex24 clones in the eye occupy a larger area under a protein-poor diet than under standard diet.
unkex24 homozygous eye disc clone cells show accelerated cell cycle progression under a protein-poor diet, as mutant second mitotic wave cells are more anterior than neighboring wild-type controls.
Homozygous unkex24 or heterozygous unkex24/unkex13, unkex24/unke01984, or unkex24/unkDf flies are pupal lethal.
unkex24 mutant eye clones cause precocious differentiation of R1/6/7 and cone cells. unkex24 mutant clones have no effect on the differentiation of R3/4, or R2/5 cells.
unkex24 mutant clones exhibit a strong phenotype in the adult eye. Mutant ommatidia in the anterior half of the eye have a normal structure but display rotation defects. Ommatidia in the posterior half of the eye are missing photoreceptors and contain photoreceptors with elliptical and split rhabdomeres, suggesting a perturbation of the F-actin cytoskeleton.
unkex24 mutant eye disc clones are a similar size to wild-type.
unkex24 has ommatidium | somatic clone phenotype, suppressible by Rheb2D1
unkex24 has rhabdomere | somatic clone phenotype, suppressible by Rheb2D1
unkex24 is a suppressor of photoreceptor cell R1 | somatic clone phenotype of Rheb2D1
unkex24 is a suppressor of photoreceptor cell R6 | somatic clone phenotype of Rheb2D1
heca43, unkex24 has ommatidium | somatic clone phenotype
heca43, unkex24 has rhabdomere | somatic clone phenotype
heca43, unkex24 has photoreceptor cell R7 | somatic clone phenotype
heca43, unkex24 has photoreceptor cell R1 | somatic clone phenotype
heca43, unkex24 has photoreceptor cell R6 | somatic clone phenotype
unkex24, hdc43 double mutant eye clones exhibit precocious differentiation of R1/6 two to three rows ahead of the differentiation front, as in the single mutants. The double mutant has a similar adult eye phenotype to each single mutant.
unkex24, Rheb2D1 mutant eye clones display wild-type differentiation of the R1/6 photoreceptor cells, indicating unk suppresses the strong delay in differentiation exhibited in Rheb mutant clones. In the adult eye, the elliptical and split rhabdomere phenotype seen in unkex24 clones is suppressed in unkex24, Rheb2D1 mutant clones. However, these double mutant clones contain mis-rotated ommatidia and missing photoreceptors.
unkex24 is rescued by unkUAS.cARa/Scer\GAL4GMR.PU
The R1/6 precocious differentiation phenotype in unkex24 mutant clones is rescued by overexpression of unkScer\UAS.cARa.
