FlyBase Allele Report: Dmel\E2f1[164]

General Information

Symbol

Dmel\E2f1¹⁶⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0052453

Feature type

allele

Associated gene

Dmel\E2f1

Associated Insertion(s)

P{hsneo.ry⁺}E2f1¹⁶⁴

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Duronio et al., 1998)

Mutagen

P-element activity

(Seum et al., 1996)

Progenitor genotype

Associated Insertion(s)

P{hsneo.ry⁺}E2f1¹⁶⁴

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

enhancer of variegation | dominant, with In(1)w^m4h

(Seum et al., 1996)

female fertile

(Seum et al., 1996)

increased occurrence of cell division | embryonic stage (with Df(3R)e-D7)

(Duronio et al., 1998)

male fertile

(Seum et al., 1996)

non-enhancer of variegation | dominant, with P{hsp26-pt-T}39C-12

(Sun et al., 2000)

non-enhancer of variegation | dominant, with P{hsp26-pt-T}39C-42

(Sun et al., 2000)

non-enhancer of variegation | dominant, with P{hsp26-pt-T}118E-3

(Sun et al., 2000)

non-enhancer of variegation | dominant, with P{hsp26-pt-T}118E-10

(Sun et al., 2000)

non-enhancer of variegation | dominant, with P{hsp26-pt-T}118E-19

(Sun et al., 2000)

non-enhancer of variegation | dominant, with P{hsp26-pt-T}118E-25-5

(Sun et al., 2000)

non-enhancer of variegation | dominant, with P{hsp26-pt-T}Abcd1^39C-34

(Sun et al., 2000)

non-suppressor of variegation | dominant, with P{hsp26-pt-T}39C-12

(Sun et al., 2000)

non-suppressor of variegation | dominant, with P{hsp26-pt-T}39C-42

(Sun et al., 2000)

non-suppressor of variegation | dominant, with P{hsp26-pt-T}118E-3

(Sun et al., 2000)

non-suppressor of variegation | dominant, with P{hsp26-pt-T}118E-10

(Sun et al., 2000)

non-suppressor of variegation | dominant, with P{hsp26-pt-T}118E-19

(Sun et al., 2000)

non-suppressor of variegation | dominant, with P{hsp26-pt-T}118E-25-5

(Sun et al., 2000)

non-suppressor of variegation | dominant, with P{hsp26-pt-T}Abcd1^39C-34

(Sun et al., 2000)

viable

(Du, 2000, Duronio et al., 1998, Seum et al., 1996)

Phenotype Manifest In

anterior midgut primordium | embryonic stage (with Df(3R)e-D7)

(Duronio et al., 1998)

midgut primordium & S phase

(Duronio et al., 1998)

Detailed Description

Statement

Reference

The pattern of BrdU incorporation in E2f⁹¹/E2f¹⁶⁴ eye discs is the same as wild type.

(Du, 2000)

E2f¹⁶⁴/Df(3R)e-D7 adults are viable and fertile. The pattern of BrdU incorporation suggests that S phase is prolonged in the midgut of E2f¹⁶⁴/Df(3R)e-D7 embryos.

(Duronio et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

E2f1¹⁶⁴/E2f1⁹¹ is a suppressor of lethal | recessive phenotype of Rbf¹⁴

(Du, 2000)

Other

Statement

Reference

E2f1¹⁶⁴/E2f1⁹¹, Rbf¹⁴/Rbf^120a has abnormal developmental rate | larval stage phenotype

(Du, 2000)

E2f1¹⁶⁴/E2f1⁹¹, Rbf¹⁴/Rbf^120a has increased cell death | larval stage phenotype

(Du, 2000)

E2f1¹⁶⁴/E2f1⁹¹, Rbf¹⁴/Rbf^120a has abnormal size | larval stage phenotype

(Du, 2000)

E2f1¹⁶⁴/E2f1⁹¹, Rbf¹⁴/Rbf^120a has abnormal body size | larval stage phenotype

(Du, 2000)

E2f1¹⁶⁴/E2f1⁹¹, Rbf¹⁴/Rbf^120a has increased occurrence of cell division | larval stage phenotype

(Du, 2000)

Phenotype Manifest In

Suppressor of

Statement

Reference

E2f1¹⁶⁴/E2f1⁹¹ is a suppressor of scutellar bristle phenotype of Rbf¹⁴

(Du, 2000)

E2f1¹⁶⁴/E2f1⁹¹ is a suppressor of eye phenotype of Rbf¹⁴

(Du, 2000)

E2f1¹⁶⁴/E2f1⁹¹ is a suppressor of ommatidium phenotype of Rbf¹⁴

(Du, 2000)

Other

Statement

Reference

E2f1¹⁶⁴/E2f1⁹¹, Rbf¹⁴ has eye disc phenotype

(Du, 2000)

E2f1¹⁶⁴/E2f1⁹¹, Rbf¹⁴ has eye disc & mitotic cell cycle phenotype

(Du, 2000)

E2f1¹⁶⁴/E2f1⁹¹, Rbf¹⁴/Rbf^120a has eye disc morphogenetic furrow | larval stage phenotype

(Du, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The early larval lethality of Rbf¹⁴/Y males is partially suppressed by E2f⁹¹/E2f¹⁶⁴; these larvae survive to the third larval instar stage. Their development is delayed by about 2 days compared to wild type, and most of the larvae can develop further into pupae. The Rbf¹⁴/Y ; E2f⁹¹/E2f¹⁶⁴ larvae and pupae are slightly smaller than wild type. Some develop into very late stage pupae with adult structures (such as eyes, legs, wings, cuticle and bristles) but they cannot completely eclose. Adult Rbf¹⁴ flies rescued by expressing Rbf^hs.PD during the first 3 days after egg laying show a number of alterations in adult structures such as abnormal macrochaetae on the notum and rough eyes. These adult phenotypes are also rescued without heat shock if the flies are also carrying E2f⁹¹/E2f¹⁶⁴. There are two differences in the pattern of BrdU incorporation in the eye discs of Rbf¹⁴/Y ; E2f⁹¹/E2f¹⁶⁴ larvae compared to wild type. Firstly, the G₁ arrested region in the morphogenetic furrow is much narrower, cells just anterior to the morphogenetic furrow are not arrested in G₁, instead they become part of the first mitotic wave. Secondly, some cells posterior to the second mitotic wave still incorporate BrdU. Rbf¹⁴/Y ; E2f⁹¹/E2f¹⁶⁴ eye discs are slightly smaller than normal. There is increased cell death (seen just anterior to the morphogenetic furrow) in these eye discs compared to those of wild-type or E2f⁹¹/E2f¹⁶⁴ larvae.

(Du, 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Mobilisation of the insertion results in eight internal deletions of the P-element and three precise excisions. Precise excision reverts the enhanced In(1)w^m4h variegation phenotype.

(Seum et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

E(var)3-93E

(Sun et al., 2000)

E(var)3-93E¹⁶⁴

(Seum et al., 1996)

E2f1¹⁶⁴

E2f¹⁶⁴

dE2F¹⁶⁴

(Duronio et al., 1998)

de2f1¹⁶⁴

(Du, 2000)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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