FlyBase Allele Report: Dmel\mir-11[UAS.DsRed2]

General Information

Symbol

Dmel\mir-11^UAS.DsRed2

Species

D. melanogaster

Name

FlyBase ID

FBal0178871

Feature type

allele

Associated gene

Dmel\mir-11

Associated Insertion(s)

Carried in Construct

P{UAS-DsRed2-mir-11}

Also Known As

UAS-miR-11

Key Links

Transgenic product class

wild_type

(Brennecke et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Brennecke et al., 2005)

Mutagen

in vitro construct

(Brennecke et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-DsRed2-mir-11}

Cytology

Description

UAS sequences drive expression of DsRed2 followed by a genomic fragment that includes mir-11.

(Brennecke et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-11

Tagged with

DsRed2

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage, with Scer\GAL4^da.G32

(Bejarano et al., 2012)

some die during embryonic stage, with Scer\GAL4^da.G32

(Bejarano et al., 2012)

visible, with Scer\GAL4^Bx-MS1096

(Bejarano et al., 2012)

visible, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012)

Phenotype Manifest In

crossvein, with Scer\GAL4^Bx-MS1096

(Bejarano et al., 2012)

wing, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012)

wing vein L5, with Scer\GAL4^Bx-MS1096

(Bejarano et al., 2012)

Detailed Description

Statement

Reference

Expression of mir-11^{Scer\UAS.T:Disc\RFP-DsRed2} under the control of Scer\GAL4^ptc-559.1 does not result in a mutant phenotype.

(Bejarano et al., 2012)

Expression of mir-11^{Scer\UAS.T:Disc\RFP-DsRed2} under the control of Scer\GAL4^Bx-MS1096 results in loss of the crossveins and wing vein L5.

(Bejarano et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

mir-11^UAS.DsRed2/Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a suppressor of increased cell death phenotype of E2f1^UAS.cNa

(Truscott et al., 2014)

mir-11^UAS.DsRed2, Scer\GAL4^GMR.PU is a suppressor of increased cell death phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PU

(Truscott et al., 2014)

Scer\GAL4^sca.PU/mir-11^UAS.DsRed2 is a suppressor of visible phenotype of Scer\GAL4^sca.PU, mi^GD11624

(Szuplewski et al., 2012)

mir-11^UAS.DsRed2, Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PF

(Truscott et al., 2011)

mir-11^UAS.DsRed2, Scer\GAL4^Act88F.PD is a suppressor of visible phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Truscott et al., 2011)

NOT Suppressor of

Statement

Reference

mir-11^UAS.DsRed2/Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a non-suppressor of increased cell death phenotype of Rbf^120a

(Truscott et al., 2014)

mir-11^UAS.DsRed2, Scer\GAL4^Act88F.PD is a non-suppressor of visible phenotype of Cele\ced-3^UAS.cSa, Scer\GAL4^Act88F.PD

(Truscott et al., 2011)

mir-11^UAS.DsRed2, Scer\GAL4^GMR.PF is a non-suppressor of abnormal cell cycle phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PF

(Truscott et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

mir-11^UAS.DsRed2/Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a suppressor of wing phenotype of E2f1^UAS.cNa

(Truscott et al., 2014)

mir-11^UAS.DsRed2, Scer\GAL4^GMR.PU is a suppressor of eye disc phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PU

(Truscott et al., 2014)

Scer\GAL4^sca.PU/mir-11^UAS.DsRed2 is a suppressor of macrochaeta phenotype of Scer\GAL4^sca.PU, mi^GD11624

(Szuplewski et al., 2012)

mir-11^UAS.DsRed2, Scer\GAL4^GMR.PF is a suppressor of eye disc phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PF

(Truscott et al., 2011)

mir-11^UAS.DsRed2, Scer\GAL4^Act88F.PD is a suppressor of wing blade phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Truscott et al., 2011)

NOT Suppressor of

Statement

Reference

mir-11^UAS.DsRed2/Scer\GAL4^{FRT.Rnor\Cd2.Act5C} is a non-suppressor of eye disc phenotype of Rbf^120a

(Truscott et al., 2014)

mir-11^UAS.DsRed2, Scer\GAL4^Act88F.PD is a non-suppressor of wing blade phenotype of Cele\ced-3^UAS.cSa, Scer\GAL4^Act88F.PD

(Truscott et al., 2011)

mir-11^UAS.DsRed2, Scer\GAL4^GMR.PF is a non-suppressor of eye disc phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PF

(Truscott et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of mir-11^{Scer\UAS.T:Disc\RFP-DsRed2} suppresses the apoptosis seen in the wings of newly eclosed flies expressing E2f1^Scer\UAS.cNa under the control of Scer\GAL4^Act88F.PD.

Generation of eye disc clones expressing mir-11^{Scer\UAS.T:Disc\RFP-DsRed2} does not suppress the cell death seen in Rbf^120a mutant eye discs.

Expression of mir-11^{Scer\UAS.T:Disc\RFP-DsRed2} suppresses the apoptosis seen in the eye disc in flies expressing E2f1^Scer\UAS.cNa and Dp^Scer\UAS.cDa under the control of Scer\GAL4^GMR.PU.

(Truscott et al., 2014)

The addition of mir-11^{Scer\UAS.T:Disc\RFP-DsRed2} does not prevent the ectopic S phases which are induced posterior to the morphogenetic furrow by co-expression of E2f^Scer\UAS.cNa and Dp^Scer\UAS.cDa under the control of Scer\GAL4^GMR.PF. However, co-expression of mir-11^{Scer\UAS.T:Disc\RFP-DsRed2} does suppress the ability of co-expression of E2f^Scer\UAS.cNa and Dp^Scer\UAS.cDa under the control of Scer\GAL4^GMR.PF to induce apoptosis in the eye disc.

Co-expression of mir-11^{Scer\UAS.T:Disc\RFP-DsRed2} strongly suppresses the wing defects caused by expression of E2f^Scer\UAS.cNa under the control of Scer\GAL4^Act88F.PD.

(Truscott et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

59864

w^*; P{UAS-DsRed2-mir-11}2

59865

w^*; P{UAS-DsRed2-mir-11}3/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mir-11^{Scer\UAS.T:Disc\RFP-DsRed2}

mir-11^Scer\UAS.cBa

mir-11^UAS.DsRed2

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

Open Close