FlyBase Allele Report: Hsap\CDKN1A[GMR.PH]

General Information

Symbol

Hsap\CDKN1A^GMR.PH

Species

H. sapiens

Name

glass multimer reporter construct of Hariharan

FlyBase ID

FBal0047094

Feature type

allele

Associated gene

Hsap\CDKN1A

Associated Insertion(s)

Carried in Construct

P{GMRp21^B}

Also Known As

GMR-p21, GMRp21

Key Links

Transgenic product class

cDNA

(de Nooij and Hariharan, 1995)

wild_type

(de Nooij and Hariharan, 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(de Nooij and Hariharan, 1995)

wild_type

(de Nooij and Hariharan, 1995)

Mutagen

in vitro construct

(Flores et al., 1998, Secombe et al., 1998, de Nooij and Hariharan, 1995)

Progenitor genotype

Carried in construct

P{GMRp21^B}

Cytology

Description

GMR regulatory sequences drive expression of a Hsap\CDKN1A cDNA.

(de Nooij and Hariharan, 1995)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Hsap\CDKN1A

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage

(Quinn et al., 2004)

decreased occurrence of cell division | larval stage

(Staehling-Hampton et al., 1999, Secombe et al., 1998)

visible

(Fan et al., 2010, Du and Dyson, 1999, Staehling-Hampton et al., 1999, Secombe et al., 1998)

visible | adult stage

(Li et al., 2021)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Hsap\CDKN1A^GMR.PH in the developing eye results in ommatidial fusion.

(Morishita et al., 2013)

Expression of Hsap\CDKN1A^GMR.PH produces flies with rough eyes. Little or no apoptosis is seen in third instar eye discs and differentiation of photoreceptors is largely normal.

(Fan et al., 2010)

Hsap\CDKN1A^GMR.PH flies have small, rough eyes.

(Quinn et al., 2004)

Flies expressing Hsap\CDKN1A^GMR.PH have rough eyes. The second mitotic wave is absent in eye imaginal discs expressing Hsap\CDKN1A^GMR.PH.

(Staehling-Hampton et al., 1999)

Flies expressing Hsap\CDKN1A^GMR.PH have a severe rough eye phenotype. The number of cells entering S phase posterior to the morphogenetic furrow is severely reduced compared to wild-type.

(Secombe et al., 1998)

Ommatidial architecture is irregular, and interommatidial bristle number is reduced to 15% of wild type. Almost all (more than 98%) of ommatidia have the full complement of photoreceptor cells, though only 13% were fully surrounded by pigment cells.

(de Nooij and Hariharan, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\CDKN1A^GMR.PH has abnormal size | adult stage phenotype, enhanceable by Myc^P0/Myc[+]

(Quinn et al., 2004)

Hsap\CDKN1A^GMR.PH has decreased occurrence of cell division | larval stage phenotype, enhanceable by CycE^JP

(Secombe et al., 1998)

Hsap\CDKN1A^GMR.PH has visible | larval stage phenotype, enhanceable by CycE^JP

(Secombe et al., 1998)

Suppressed by

Statement

Reference

Hsap\CDKN1A^GMR.PH has abnormal size | adult stage phenotype, suppressible by hfp^EP3058/hfp[+]

(Quinn et al., 2004)

Hsap\CDKN1A^GMR.PH has visible phenotype, suppressible by Df(1)AD11

(Du and Dyson, 1999)

Hsap\CDKN1A^GMR.PH has visible phenotype, suppressible by Df(1)su(s)83

(Du and Dyson, 1999)

Hsap\CDKN1A^GMR.PH has visible phenotype, suppressible by Rbf^120a/Rbf[+]

(Du and Dyson, 1999)

Hsap\CDKN1A^GMR.PH has visible phenotype, suppressible by Rbf¹⁴/Rbf[+]

(Du and Dyson, 1999)

Hsap\CDKN1A^GMR.PH has visible phenotype, suppressible by spen^poc361

(Staehling-Hampton et al., 1999)

Hsap\CDKN1A^GMR.PH has decreased occurrence of cell division | larval stage phenotype, suppressible by spen^poc361

(Staehling-Hampton et al., 1999)

Enhancer of

Statement

Reference

Hsap\CDKN1A^GMR.PH is an enhancer of visible | recessive phenotype of CycE^JP

(Secombe et al., 1998)

Hsap\CDKN1A^GMR.PH is an enhancer of decreased occurrence of cell division | larval stage phenotype of CycE^JP

(Secombe et al., 1998)

NOT Enhancer of

Statement

Reference

Hsap\CDKN1A^GMR.PH is a non-enhancer of abnormal eye color | dominant phenotype of Acf^EY08629, Scer\GAL4^GMR.PF, bw^D

(Schneiderman et al., 2010)

Suppressor of

Statement

Reference

Hsap\CDKN1A^GMR.PH is a suppressor of increased cell death | third instar larval stage phenotype of p53^GMR.Ex

(Fan et al., 2010)

Hsap\CDKN1A^GMR.PH is a suppressor of decreased cell number | third instar larval stage phenotype of p53^GMR.Ex

(Fan et al., 2010)

Hsap\CDKN1A^GMR.PH is a suppressor of abnormal size | adult stage phenotype of p53^GMR.Ex

(Fan et al., 2010)

Hsap\CDKN1A^GMR.PH is a suppressor of visible | adult stage phenotype of p53^GMR.Ex

(Fan et al., 2010)

Hsap\CDKN1A^GMR.PH is a suppressor of abnormal eye color | dominant phenotype of Scer\GAL4^GMR.PF, bw^D, crol^unspecified

(Schneiderman et al., 2010)

Hsap\CDKN1A^GMR.PH is a suppressor of abnormal eye color | dominant phenotype of Scer\GAL4^GMR.PF, bw^D, mir-ban^unspecified

(Schneiderman et al., 2010)

NOT Suppressor of

Statement

Reference

Hsap\CDKN1A^GMR.PH is a non-suppressor of abnormal eye color | dominant phenotype of Acf^EY08629, Scer\GAL4^GMR.PF, bw^D

(Schneiderman et al., 2010)

Hsap\CDKN1A^GMR.PH is a non-suppressor of increased cell death | larval stage phenotype of Egfr^E3

(Baker and Yu, 2001)

Other

Statement

Reference

Hsap\CDKN1A^GMR.PH, hfp^EP3058/hfp[+] has increased occurrence of cell division | larval stage phenotype

(Quinn et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\CDKN1A^GMR.PH has ommatidium phenotype, enhanceable by dm[+]/Myc^P0

(Quinn et al., 2004)

Hsap\CDKN1A^GMR.PH has eye phenotype, enhanceable by dm[+]/Myc^P0

(Quinn et al., 2004)

Hsap\CDKN1A^GMR.PH has eye disc posterior to the morphogenetic furrow | larval stage phenotype, enhanceable by CycE^JP

(Secombe et al., 1998)

Hsap\CDKN1A^GMR.PH has eye phenotype, enhanceable by CycE^JP

(Secombe et al., 1998)

NOT Enhanced by

Statement

Reference

Hsap\CDKN1A^GMR.PH has phenotype, non-enhanceable by osa^E65

(Staehling-Hampton et al., 1999)

Suppressed by

Statement

Reference

Hsap\CDKN1A^GMR.PH has ommatidium phenotype, suppressible by pUf68[+]/hfp^EP3058

(Quinn et al., 2004)

Hsap\CDKN1A^GMR.PH has eye phenotype, suppressible by pUf68[+]/hfp^EP3058

(Quinn et al., 2004)

Hsap\CDKN1A^GMR.PH has eye disc & mitotic cell cycle phenotype, suppressible by CycE^GMR.PB

(Boulton et al., 2000)

Hsap\CDKN1A^GMR.PH has eye disc & mitotic cell cycle phenotype, suppressible by ebi^unspecified/ebi[+]

(Boulton et al., 2000)

Hsap\CDKN1A^GMR.PH has eye phenotype, suppressible by ebi^unspecified/ebi[+]

(Boulton et al., 2000)

Hsap\CDKN1A^GMR.PH has interommatidial bristle phenotype, suppressible by ebi^unspecified/ebi[+]

(Boulton et al., 2000)

Hsap\CDKN1A^GMR.PH has ommatidium phenotype, suppressible by ebi^unspecified/ebi[+]

(Boulton et al., 2000)

Hsap\CDKN1A^GMR.PH has eye phenotype, suppressible by Df(1)AD11

(Du and Dyson, 1999)

Hsap\CDKN1A^GMR.PH has eye phenotype, suppressible by Df(1)su(s)83

(Du and Dyson, 1999)

Hsap\CDKN1A^GMR.PH has eye phenotype, suppressible by Rbf^120a/Rbf[+]

(Du and Dyson, 1999)

Hsap\CDKN1A^GMR.PH has eye phenotype, suppressible by Rbf¹⁴/Rbf[+]

(Du and Dyson, 1999)

Hsap\CDKN1A^GMR.PH has eye phenotype, suppressible by spen^poc361

(Staehling-Hampton et al., 1999)

Hsap\CDKN1A^GMR.PH has ommatidium phenotype, suppressible by spen^poc361

(Staehling-Hampton et al., 1999)

Hsap\CDKN1A^GMR.PH has eye disc phenotype, suppressible by spen^poc361

(Staehling-Hampton et al., 1999)

NOT suppressed by

Statement

Reference

Hsap\CDKN1A^GMR.PH has eye phenotype, non-suppressible by Cdk7^S164A.T170A

(Morishita et al., 2013)

Hsap\CDKN1A^GMR.PH has ommatidium phenotype, non-suppressible by Cdk7^S164A.T170A

(Morishita et al., 2013)

Hsap\CDKN1A^GMR.PH has eye disc & mitotic cell cycle phenotype, non-suppressible by ttk^88.hs

(Boulton et al., 2000)

Hsap\CDKN1A^GMR.PH has phenotype, non-suppressible by Diap1^SL

(Lisi et al., 2000)

Enhancer of

Statement

Reference

Hsap\CDKN1A^GMR.PH is an enhancer of eye phenotype of CycE^JP

(Secombe et al., 1998)

Hsap\CDKN1A^GMR.PH is an enhancer of eye disc posterior to the morphogenetic furrow | larval stage phenotype of CycE^JP

(Secombe et al., 1998)

NOT Enhancer of

Statement

Reference

Hsap\CDKN1A^GMR.PH is a non-enhancer of eye phenotype of Acf^EY08629, Scer\GAL4^GMR.PF, bw^D

(Schneiderman et al., 2010)

Suppressor of

Statement

Reference

Hsap\CDKN1A^GMR.PH is a suppressor of eye disc | posterior | third instar larval stage phenotype of p53^GMR.Ex

(Fan et al., 2010)

Hsap\CDKN1A^GMR.PH is a suppressor of photoreceptor cell R7 | third instar larval stage phenotype of p53^GMR.Ex

(Fan et al., 2010)

Hsap\CDKN1A^GMR.PH is a suppressor of eye phenotype of p53^GMR.Ex

(Fan et al., 2010)

Hsap\CDKN1A^GMR.PH is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, bw^D, crol^unspecified

(Schneiderman et al., 2010)

Hsap\CDKN1A^GMR.PH is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, bw^D, mir-ban^unspecified

(Schneiderman et al., 2010)

NOT Suppressor of

Statement

Reference

Hsap\CDKN1A^GMR.PH is a non-suppressor of eye phenotype of Acf^EY08629, Scer\GAL4^GMR.PF, bw^D

(Schneiderman et al., 2010)

Hsap\CDKN1A^GMR.PH is a non-suppressor of eye disc | larval stage phenotype of Egfr^E3

(Baker and Yu, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

A Cdk7^S164A.T170A mutant background does not suppress the ommatidial fusion found upon expression of Hsap\CDKN1A^GMR.PH in the developing eye.

(Morishita et al., 2013)

Expression of Hsap\CDKN1A^GMR.PH rescues the cell death and R7 photoreceptor loss seen in p53^GMR.Ex third instar larval eye discs. The adult eye ablation phenotype is also rescued.

(Fan et al., 2010)

The increased eye pigmentation in bw^D/+ heterozygotes resulting from the mis-expression of Acf1^EY08629 driven by Scer\GAL4^GMR.PF is not affected by Hsap\CDKN1A^GMR.PH.

The increased eye pigmentation in bw^D/+ heterozygotes resulting from the mis-expression of ban^unspecified driven by Scer\GAL4^GMR.PF is suppressed by Hsap\CDKN1A^GMR.PH.

The increased eye pigmentation in bw^D/+ heterozygotes resulting from the mis-expression of crol^unspecified driven by Scer\GAL4^GMR.PF is suppressed by Hsap\CDKN1A^GMR.PH.

(Schneiderman et al., 2010)

The small, rough eye phenotype of Hsap\CDKN1A^GMR.PH flies is partially suppressed by pUf68^EP3058/+ (the eyes are larger and contain fewer fused ommatidia). Compared with Hsap\CDKN1A^GMR.PH, eye discs from Hsap\CDKN1A^GMR.PH; , pUf68^EP3058/+ animals have more S-phase cells posterior to the morphogenetic furrow, and an accompanying increase in mitotic cells. The small, rough eye phenotype of Hsap\CDKN1A^GMR.PH flies is enhanced by dm^P0/+.

The small, rough eye phenotype of Hsap\CDKN1A^GMR.PH flies is partially suppressed by hfp^EP3058/+ (the eyes are larger and contain fewer fused ommatidia). Compared with Hsap\CDKN1A^GMR.PH, eye discs from Hsap\CDKN1A^GMR.PH; hfp^EP3058/+ animals have more S-phase cells posterior to the morphogenetic furrow, and an accompanying increase in mitotic cells. The small, rough eye phenotype of Hsap\CDKN1A^GMR.PH flies is enhanced by Myc^P0/+.

(Quinn et al., 2004)

Expression of Hsap\CDKN1A^GMR.PH in homozygous Egfr^E3 mutants does not prevent the apoptosis of undifferentiated cells in the eye disc that is seen in the Egfr^E3 mutant background.

(Baker and Yu, 2001)

The Hsap\CDKN1A^GMR.PH eye phenotype is dominantly suppressed by the addition of ebi^unspecified. The resulting eyes are almost wild-type, showing minor bristle defects. ebi^unspecified also partially rescues the loss of the second mitotic wave usually seen in Hsap\CDKN1A^GMR.PH eye discs.

(Boulton et al., 2000)

The eye phenotype produced by Hsap\CDKN1A^GMR.PH is strongly suppressed by Df(1)AD11, Df(1)su(s)83, Rbf^120a or Rbf¹⁴.

(Du and Dyson, 1999)

The Hsap\CDKN1A^GMR.PH rough eye phenotype is suppressed by spen^poc361. The second mitotic wave is partially restored in Hsap\CDKN1A^GMR.PH flies the presence of one copy of spen^poc361.

(Staehling-Hampton et al., 1999)

Hsap\CDKN1A^GMR.PH;CycE^JP double mutant flies have a more severe rough eye phenotype and greater reduction in the number of cells entering S phase posterior to the morphogenetic furrow than either Hsap\CDKN1A^GMR.PH or CycE^JP single mutant flies.

(Secombe et al., 1998)

Expression of Hsap\CDKN1A^GMR.PH, which blocks the second mitotic wave, in Gl¹ mutants substantially reduces the number of mispositioned nuclei. Expression also causes substantial cell death behind the morphogenetic furrow in Gl¹ mutants.

(Fan and Ready, 1997)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\CDKN1A^GMR.PH

glass-p21

(Lu et al., 1998)

Name Synonyms

glass multimer reporter construct of Hariharan

(de Nooij and Hariharan, 1995)

Secondary FlyBase IDs

References (20)

Report Sections

Open Close