FlyBase Allele Report: Dmel\dap[4]

General Information

Symbol

Dmel\dap⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0057657

Feature type

allele

Associated gene

Dmel\dap

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Lane et al., 1996)

Mutagen

P-element activity

(Lane et al., 1996)

Progenitor genotype

dap¹

(Lane et al., 1996)

Cytology

Description

Intragenic deletion encompassing sequences encoding the cyclin/cdk binding domain.

(Lane et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval optic lobe | third instar larval stage

(Wallace et al., 2000)

embryonic/larval tracheal dorsal trunk

(Beitel and Krasnow, 2000)

embryonic/larval tracheal transverse connective

(Beitel and Krasnow, 2000)

external sensory organ precursor cell IIIb & S phase

(Audibert et al., 2005)

karyosome | germline clone

(Hong et al., 2003)

lch1 neuron

(zur Lage and Jarman, 2010)

mitotic cell cycle | ectopic

(Lane et al., 1996)

oocyte | germline clone

(Hong et al., 2003)

PNS glial cell & S phase

(Audibert et al., 2005)

Detailed Description

Statement

Reference

Homozygous embryos have duplicated of the v'ch1 neurons.

(zur Lage and Jarman, 2010)

The cell division index of homozygous female germline stem cell clones does not change under poor food conditions (in contrast to wild-type clones, where the cell division is dramatically reduced under poor food conditions).

(Yu et al., 2009)

In dap⁴ mutants, an S-phase can be aberrantly induced late in telophase in 100% of the pIIIb cells and 93% of the glial cells arising from the pIIb division.

(Audibert et al., 2005)

Over 80% of dap⁴ mutant oocytes (from germ-line clones) enter endocycle and become polyploid. In over 50% of cases the extent of polyploidy makes the oocyte indistinguishable from nurse cells. In the remaining cases DNA content is somewhat lower than in nurse cells. In the cases where these mutant oocytes do not become obviously polyploid, karyosome formation is often aberrant. A small percentage of dap⁴ germ-line mutant egg chambers contain more than nurse cell like germ cells. Marker experiments show that oocyte specification occurs normally in dap⁴ germ-line clones, but that oocyte identity is not maintained in mutant oocytes that become polyploid. Consistent with this, yolk uptake is only seen in non-polyploid mutant oocytes.

(Hong et al., 2003)

Some branches of the tracheal system have nearly twice the normal number of tracheal cells in mutant embryos. Despite the increased cell number, primary branching occurs normally and branch sizes do not increase.

(Beitel and Krasnow, 2000)

Homozygous dap⁴ mutant larvae exhibit overproliferation of the cells of the optic lobe. There is a significant increase in the number of mitotically active cells and breakdown of mitotic domains, as compared to wild type.s

(Wallace et al., 2000)

One extra cycle of ectopic proliferation in the epidermis.

(Lane et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

dap⁴ is a non-enhancer of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

dap⁴ is a non-enhancer of visible phenotype of Hsap\TP53^UAS.cYa, Scer\GAL4^GMR.PS

(Yamaguchi et al., 1999)

Suppressor of

Statement

Reference

dap[+]/dap⁴ is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^en-e16E, Skp2^KK108837

(Dui et al., 2013)

dap[+]/dap⁴ is a suppressor of abnormal size phenotype of Dcr-1^GD11429, Scer\GAL4^en-e16E

(Herranz et al., 2010)

dap[+]/dap⁴ is a suppressor of decreased cell growth rate phenotype of Dcr-1^GD11429, Scer\GAL4^en-e16E

(Herranz et al., 2010)

dap[+]/dap⁴ is a suppressor | partially of decreased occurrence of cell division | germline clone phenotype of InR^ex52.1

(Yu et al., 2009)

dap[+]/dap⁴ is a suppressor of visible phenotype of Scer\GAL4^en-e16E, pzg^RNAi.UAS

(Kugler and Nagel, 2007)

dap[+]/dap⁴ is a suppressor of decreased occurrence of cell division phenotype of CycE^UAS.cLa, Scer\GAL4^neur-P72

(Audibert et al., 2005)

dap[+]/dap⁴ is a suppressor of decreased occurrence of cell division | germline clone | oogenesis phenotype of Dcr-1^Q1147X

(Hatfield et al., 2005)

dap⁴ is a suppressor of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

dap⁴ is a suppressor of visible | recessive phenotype of CycE^JP

(Secombe et al., 1998)

NOT Suppressor of

Statement

Reference

dap⁴ is a non-suppressor of visible phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

dap⁴ is a non-suppressor of abnormal size phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

dap⁴ is a non-suppressor of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

dap⁴ is a non-suppressor of abnormal mitotic cell cycle | embryonic stage phenotype of CycA^C8LR1

(Reber et al., 2006)

dap⁴ is a non-suppressor of visible phenotype of Hsap\TP53^UAS.cYa, Scer\GAL4^GMR.PS

(Yamaguchi et al., 1999)

Other

Statement

Reference

Rbf¹⁴, dap⁴ has abnormal mitotic cell cycle | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

CycD¹, dap[+]/dap⁴ has viable phenotype

(Emmerich et al., 2004)

CycD², dap[+]/dap⁴ has viable phenotype

(Emmerich et al., 2004)

CycD¹, dap⁴ has viable phenotype

(Emmerich et al., 2004)

CycD², dap⁴ has viable phenotype

(Emmerich et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

dap⁴ has PNS glial cell phenotype, suppressible by Scer\GAL4^neur-P72/CycE^UAS.cLa

(Audibert et al., 2005)

NOT Enhancer of

Statement

Reference

dap⁴ is a non-enhancer of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Suppressor of

Statement

Reference

dap[+]/dap⁴ is a suppressor | partially of wing hair phenotype of Scer\GAL4^en-e16E, Skp2^KK108837

(Dui et al., 2013)

dap[+]/dap⁴ is a suppressor of wing blade phenotype of Dcr-1^GD11429, Scer\GAL4^en-e16E

(Herranz et al., 2010)

dap[+]/dap⁴ is a suppressor | partially of female germline stem cell | germline clone phenotype of InR^ex52.1

(Yu et al., 2009)

dap[+]/dap⁴ is a suppressor of wing phenotype of Scer\GAL4^en-e16E, pzg^RNAi.UAS

(Kugler and Nagel, 2007)

dap[+]/dap⁴ is a suppressor of germline stem cell | germline clone phenotype of Dcr-1^Q1147X

(Hatfield et al., 2005)

dap[+]/dap⁴ is a suppressor of female germline cyst | germline clone | oogenesis phenotype of Dcr-1^Q1147X

(Hatfield et al., 2005)

dap⁴ is a suppressor of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

dap[+]/dap⁴ is a suppressor of nurse cell phenotype of CycE⁰¹⁶⁷²

(Hong et al., 2003)

dap⁴ is a suppressor of eye phenotype of CycE^JP

(Secombe et al., 1998)

NOT Suppressor of

Statement

Reference

dap⁴ is a non-suppressor of eye phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

dap⁴ is a non-suppressor of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

dap⁴ is a non-suppressor of embryonic epidermis phenotype of CycA^C8LR1

(Reber et al., 2006)

Other

Statement

Reference

Rbf¹⁴, dap⁴ has eye disc | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of dap⁴ partially suppresses the wing hair spacing phenotype seen when Skp2^KK108837 is expressed under the control of Scer\GAL4^en-e16E.

(Dui et al., 2013)

Heterozygous dap⁴ restores cell and wing size in animals expressing Scer\GAL4^en-e16E>Dcr-1^GD11429.

(Herranz et al., 2010)

The reduced cell division index of InR^ex52.1 female germline stem cell clones can be partially rescued by dap⁴/+.

(Yu et al., 2009)

dap⁴/+ suppresses the reduction of wing area phenotype seen in flies expressing pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E.

(Kugler and Nagel, 2007)

dap⁴ does not protect Df(1)su(s)R194/+ clones in the eye; Df(1)su(s)R194/+ ; dap⁴ clones are not recovered in the adult eye in animals with mosaic eyes containing two genotypes of cells with respect to RpL36; cells which are Df(1)su(s)R194/+ and cells in which the haplo-insufficiency of Df(1)su(s)R194/+ for RpL36 has been rescued by RpL36^+t4 (in a wild-type background the Df(1)su(s)R194/+ clones are eliminated by cell competition and are not seen in the adult eye in these animals). Also, dap⁴ does not prevent apoptosis of Df(1)su(s)R194/+ cells in the wing.

(Tyler et al., 2007)

The mitotic embryonic phenotype of CycA^C8LR1 single mutants, in which mitosis 16 does not occur, is not suppressed in dap⁴; CycA^C8LR1 double mutants. However, the double mutants do enter another S phase after the stage at which mitosis occurs in equivalent wild-type embryos.

(Reber et al., 2006)

Expression of CycE^Scer\UAS.cLa under the control of Scer\GAL4^neur-P72, in a dap⁴/+ background, induces an S-phase in 100% of pIIIb cells and 35% of sibling glial cells.

(Audibert et al., 2005)

Eye disc precluster cells mutant for both dap⁴ and Rbf¹⁴ continue to cycle instead of arresting. In addition, no G1 arrest occurs anterior to the morphogenetic furrow, all cells enter the second mitotic wave, and cells continue to cycle more posteriorly.

(Firth and Baker, 2005)

When dap⁴/+ and homozygous Dcr-1^Q1147X are combined a partial rescue of the cyst production phenotype seen Dcr-1^Q1147X mutants is seen.

(Hatfield et al., 2005)

Suppresses the rough eye phenotype of CycE^JP homozygotes.

(Secombe et al., 1998)

Xenogenetic Interactions

Statement

Reference

dap⁴ does not suppress the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

(Gupta et al., 2008)

Complementation and Rescue Data

Rescued by

dap⁴ is rescued by dap^1gm.Tag:MYC

(Meyer et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6639

w^*; dap⁴/CyO, P{ftz-lacB}E3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

(Mukherjee et al., 2006)

dap⁴

(Wang and Baker, 2015, Dui et al., 2013, Herranz et al., 2010, zur Lage and Jarman, 2010, Yu et al., 2009, Caussinus et al., 2008, Gupta et al., 2008, Kugler and Nagel, 2007, Tyler et al., 2007, Reber et al., 2006, Firth and Baker, 2005)

dap⁶

(Wallace et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (27)

Report Sections

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