FlyBase Allele Report: Dmel\pzg[RNAi.UAS]

General Information

Symbol

Dmel\pzg^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0216870

Feature type

allele

Associated gene

Dmel\pzg

Associated Insertion(s)

Carried in Construct

P{UAS-pzg.RNAi}

Also Known As

UAS-pzg-RNAi

Key Links

Transgenic product class

RNAi_reagent

(Kugler and Nagel, 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Kugler and Nagel, 2007)

Mutagen

in vitro construct

(Kugler and Nagel, 2007)

Progenitor genotype

Carried in construct

P{UAS-pzg.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of the C-terminal portion of pzg (nucleotides 2005-2704 of the cDNA sequence).

(Kugler and Nagel, 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

pzg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^da.G32

(Kugler and Nagel, 2007)

lethal | heat sensitive, with Scer\GAL4^da.G32

(Kugler and Nagel, 2007)

melanotic mass phenotype | adult stage, with Scer\GAL4^Cg.PA

(Kugler et al., 2011)

melanotic mass phenotype | adult stage, with Scer\GAL4^Hml.PG

(Kugler et al., 2011)

melanotic mass phenotype | larval stage, with Scer\GAL4^Cg.PA

(Kugler et al., 2011)

melanotic mass phenotype | larval stage, with Scer\GAL4^Hml.PG

(Kugler et al., 2011)

melanotic mass phenotype | pupal stage, with Scer\GAL4^Cg.PA

(Kugler et al., 2011)

melanotic mass phenotype | pupal stage, with Scer\GAL4^Hml.PG

(Kugler et al., 2011)

visible, with Scer\GAL4^en-e16E

(Kugler et al., 2011, Kugler and Nagel, 2007)

visible, with Scer\GAL4^ey.PH

(Kugler and Nagel, 2007)

visible, with Scer\GAL4^sd-SG29.1

(Kugler and Nagel, 2007)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Kugler and Nagel, 2007)

melanotic mass | adult stage, with Scer\GAL4^Cg.PA

(Kugler et al., 2011)

melanotic mass | adult stage, with Scer\GAL4^Hml.PG

(Kugler et al., 2011)

melanotic mass | larval stage, with Scer\GAL4^Cg.PA

(Kugler et al., 2011)

melanotic mass | larval stage, with Scer\GAL4^Hml.PG

(Kugler et al., 2011)

melanotic mass | pupal stage, with Scer\GAL4^Cg.PA

(Kugler et al., 2011)

melanotic mass | pupal stage, with Scer\GAL4^Hml.PG

(Kugler et al., 2011)

wing, with Scer\GAL4^en-e16E

(Kugler et al., 2011, Kugler and Nagel, 2007)

wing, with Scer\GAL4^sd-SG29.1

(Kugler and Nagel, 2007)

Detailed Description

Statement

Reference

Expression of pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E results in a small wing phenotype.

Expression of pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Cg.PA or Scer\GAL4^Hml.PG results in melanotic tumours in larvae, pupae and adults.

(Kugler et al., 2011)

Expression of pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 at 25^oC results in late embryonic/early larval death. At 18^oC, larval development is significantly delayed with respect to growth and developmental timing and the animals eventually die at early pupal stages.

Expression of pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PH or Scer\GAL4^sd-SG29.1 results in a reduction in the size of the eye or wing respectively.

Expression of pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E results in a reduction in wing area compared to controls.

(Kugler and Nagel, 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, pzg^RNAi.UAS has visible phenotype, enhanceable by CycE[+]/CycE⁵

(Kugler and Nagel, 2007)

Scer\GAL4^en-e16E, pzg^RNAi.UAS has visible phenotype, enhanceable by PCNA^k00704/mus209[+]

(Kugler and Nagel, 2007)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, pzg^RNAi.UAS has visible phenotype, suppressible by EcR^A.UAS, Scer\GAL4^en-e16E

(Kugler et al., 2011)

Scer\GAL4^en-e16E, pzg^RNAi.UAS has visible phenotype, suppressible by EcR^B1.UAS, Scer\GAL4^en-e16E

(Kugler et al., 2011)

Scer\GAL4^en-e16E, pzg^RNAi.UAS has visible phenotype, suppressible by dap[+]/dap⁴

(Kugler and Nagel, 2007)

Scer\GAL4^en-e16E, pzg^RNAi.UAS has visible phenotype, suppressible by N^Ax-M1/N[+]

(Kugler and Nagel, 2007)

Scer\GAL4^en-e16E, pzg^RNAi.UAS has visible phenotype, suppressible by H[+]/H¹

(Kugler and Nagel, 2007)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, pzg^RNAi.UAS has abnormal mitotic cell cycle phenotype

(Kugler and Nagel, 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, pzg^RNAi.UAS has wing phenotype, enhanceable by CycE[+]/CycE⁵

(Kugler and Nagel, 2007)

Scer\GAL4^en-e16E, pzg^RNAi.UAS has wing phenotype, enhanceable by PCNA^k00704/mus209[+]

(Kugler and Nagel, 2007)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, pzg^RNAi.UAS has wing phenotype, suppressible by EcR^A.UAS, Scer\GAL4^en-e16E

(Kugler et al., 2011)

Scer\GAL4^en-e16E, pzg^RNAi.UAS has wing phenotype, suppressible by EcR^B1.UAS, Scer\GAL4^en-e16E

(Kugler et al., 2011)

Scer\GAL4^en-e16E, pzg^RNAi.UAS has wing phenotype, suppressible by dap[+]/dap⁴

(Kugler and Nagel, 2007)

Scer\GAL4^en-e16E, pzg^RNAi.UAS has wing phenotype, suppressible by N^Ax-M1/N[+]

(Kugler and Nagel, 2007)

Scer\GAL4^en-e16E, pzg^RNAi.UAS has wing phenotype, suppressible by H[+]/H¹

(Kugler and Nagel, 2007)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, pzg^RNAi.UAS has wing disc phenotype

(Kugler and Nagel, 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of EcR^A.Scer\UAS or EcR^B1.Scer\UAS significantly suppresses the small wing phenotype caused by expression of pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E.

(Kugler et al., 2011)

Scer\GAL4^ptc-559.1-mediated expression of pzg^{dsRNA.Scer\UAS} enhances the wing margin defects seen in Df(1)N-54l9 heterozygotes.

(Kugler and Nagel, 2010)

CycE⁵/+ or mus209^k00704/+ enhances the reduction of wing area phenotype seen in flies expressing pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E.

dap⁴/+ suppresses the reduction of wing area phenotype seen in flies expressing pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E.

The reduction in wing area seen in flies expressing pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E is suppressed by N^Ax-M1/+ or H¹/+.

Expression of pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 enhances the penetrance of the notched wing phenotype in Df(1)N-54l9/+ flies from 55% to 100%, and the wing notches are markedly enlarged.

(Kugler and Nagel, 2007)

Xenogenetic Interactions

Statement

Reference

Cells in the wing disc expressing pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E in a background in which they are also co-expressing BacA\p35^Scer\UAS.cHa (to prevent cell death) show strongly reduced DNA replication (as measured by BrdU incorporation) compared to controls, and the number of cells entering mitosis is strongly reduced.

(Kugler and Nagel, 2007)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pzg^RNAi.UAS

pzg^{dsRNA.Scer\UAS}

pzg^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close