FlyBase Allele Report: Dmel\CycE[01672]

General Information

Symbol

Dmel\CycE⁰¹⁶⁷²

Species

D. melanogaster

Name

FlyBase ID

FBal0059171

Feature type

allele

Associated gene

Dmel\CycE

Associated Insertion(s)

P{PZ}CycE⁰¹⁶⁷²

Carried in Construct

Also Known As

cycE¹⁶⁷²

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Lilly and Spradling, 1996)

Mutagen

P-element activity

(Lilly and Spradling, 1996)

Progenitor genotype

Associated Insertion(s)

P{PZ}CycE⁰¹⁶⁷²

Cytology

Description

The P{PZ} insertion maps to the first intron of the CycE type II maternal transcript.

(Lilly and Spradling, 1996)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:15,740,900..15,747,832

(de Nooij et al., 2000, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Secombe et al., 1998, Lilly and Spradling, 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cyst cell of ovary

(Spradling et al., 1997)

dorsal appendage

(Lilly and Spradling, 1996)

egg chamber

(Lilly and Spradling, 1996)

germinal vesicle

(Lilly and Spradling, 1996)

heterochromatin & nurse cell

(Royzman et al., 2002)

nurse cell

(Hong et al., 2003, Lilly and Spradling, 1996)

nurse cell & nucleus

(Lilly and Spradling, 1996)

oocyte

(Lilly and Spradling, 1996)

Detailed Description

Statement

Reference

In females homozygous for CycE⁰¹⁶⁷², 37+/-7% of egg chambers (n>200) contain a cell adjacent to the true oocyte with abnormally low ploidy and other oocyte-like features, and only 14 wild-type nurse cells; i.e.- one of the nurse cells is partially transformed into a second oocyte.

(Hong et al., 2003)

Mutant ovaries show an increase in heterochromatic DNA replication but no perturbations in nurse chromosome structures.

(Royzman et al., 2002)

CycE^JP/CycE⁰¹⁶⁷² flies have essentially wild-type eyes.

(Secombe et al., 1998)

Two or three of the cystocytes differentiate as oocytes.

(Spradling et al., 1997)

Nurse cell chromocenters are increased dramatically in both size and intensity. The "5-blob" nuclear stage persists in some affected nurse cells beyond endocycles 4-5, indicating retarded chromatin changes. 30% of egg chambers contained 8 instead of 16 cells: 7 nurse cells and an oocyte. 30-40% of egg chambers contain germline cysts with two germinal vesicles and 5% contain 3. Those with two germinal vesicles always include 14 nurse cells, indicating a nurse cell to oocyte transformation. The extra germinal vesicle is smaller and has a retarded development. Eggs develop one or two extra dorsal appendages, indicating that the extra germinal vesicles can provide the usual dorsalising activity. Mutant nurse cells have increased DNA content, due to the increased propagation of the (usually under-represented) heterochromatic sequences. Late replication is restored to endomitotic nurse cells (where it does not normally occur), but not to follicle cells.

(Lilly and Spradling, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

CycE⁰¹⁶⁷² has nurse cell phenotype, suppressible by dap[+]/dap⁴

(Hong et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for dap⁴ partially suppresses the 2 oocyte phenotype of CycE⁰¹⁶⁷² homozygous females (37+/-7% to 14+/-7%).

(Hong et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Excision of the P{PZ} restores female fertility and reverts the nurse cell chromocenter phenotype.

(Lilly and Spradling, 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

CycE⁰¹⁶⁷²

(Althoff et al., 2009)

CycE⁰¹⁷⁶²

(Lehner and Lane, 1997)

DmCycE⁰¹⁶⁷²

(Lilly et al., 1997)

DmcycE^PZ01672

(Secombe et al., 1998)

cycE⁰¹⁶⁷²

(Glaser et al., 1999, Morris et al., 1998)

cycE¹⁶⁷²

(Leach et al., 2000, Dej and Spradling, 1999, Spradling et al., 1997)

cycE^fs(2)01672

(de Nooij et al., 2000)

cyclinE⁰¹⁶⁷²

(Royzman et al., 2002, de Nooij and Hariharan, 1998, de Nooij and Hariharan, 1998, de Cuevas et al., 1997)

fs(2)01672

(Lilly and Spradling, 1996)

Name Synonyms

Secondary FlyBase IDs

References (19)

Report Sections

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