FlyBase Allele Report: Dmel\Hsp83[9J1]

General Information

Symbol

Dmel\Hsp83^9J1

Species

D. melanogaster

Name

FlyBase ID

FBal0049165

Feature type

allele

Associated gene

Dmel\Hsp83

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(van der Straten et al., 1997, Dickson et al., 1996)

Mutagen

ethyl methanesulfonate

(van der Straten et al., 1997, Dickson et al., 1996)

Progenitor genotype

Cytology

Description

Point mutation. Amino acid replacement: E377K. Nucleotide substitution: G1129A.

(van der Straten et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:3,195,627..3,195,627 [+]

Nucleotide change:

G3195627A

Reported nucleotide change:

G1129A

Amino acid change:

E377K | Hsp83-PA; E377K | Hsp83-PB

Reported amino acid change:

E377K

(van der Straten et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | larval stage

(Lange et al., 2000)

lethal | recessive

(Yue et al., 1999, van der Straten et al., 1997, Dickson et al., 1996)

viable (with Hsp83^e1D)

(Milton et al., 2005)

viable (with Hsp83^e3A)

(Milton et al., 2005)

viable (with Hsp83^e6A)

(Milton et al., 2005)

viable (with Hsp83^e6D)

(Milton et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

A Hsp83^9J1 background has a highly significant and reproducible effect on total phenotypic variation of scutellar and thoracic bristles. Twice as many Hsp83^9J1 mutant flies exhibit abnormal thoracic bristle numbers, with the severity of the thoracic bristle number defects increasing from deviations of at most two thoracic bristles to as many as four. The number of flies with aberrant scutellar bristle numbers increases 4 to 5-fold in Hsp83^9J1 mutants.

(Milton et al., 2006)

Hsp83^9J1 has an effect on a normally invariant bristle trait (thoracic and scutellar bristles were analysed), three thoracic bristles (anterior dorsocentral, posterior dorsocentral and humeral bristles) are affected. Hsp83^9J1 has no significant effect on the trait mean of a variable bristle trait (the sternopleural, orbital, ocellar and vibrissa and carina bristles were analysed) compared to the background strain in which the Hsp83^9J1 mutation was induced.

(Milton et al., 2005)

Hsp83^P582/Hsp83^9J1 mutants develop until larval or early pupae. Larval brains of these mutants exhibit mitotic defects. Polyploid and other aneuploid cells make up about 30% of the mitotic figures seen. About 20% of anaphases are disorganised. The centrosome cycle is also found to be impaired in these cells. There are two classes of defect. Mitotic cells are seen containing a single microtubule organising centre (MTOC) made up of one or two unsegregated centrosomes, leading to the organisation of monopolar mitotic figures. Cells are also seen in which the centrosomes appear to fail in their assembly.

(Lange et al., 2000)

Viable in transheterozygous combination with Hsp83^e1D, Hsp83^e6D, Hsp83^e3A, Hsp83^e6A or Hsp83^e4A; males are sterile and females are weakly fertile. Hsp83^9J1/Hsp83^e3A males show some defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). Some motile sperm are present. Hsp83^9J1/Hsp83^e4A males show some defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). The nuclei and nebenkern within spermatids are mostly normal (5-10% are abnormal). Individualised sperm are present, but are not motile. Hsp83^9J1/Hsp83^e6A and Hsp83^e1D/Hsp83^9J1 males show defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile. Hsp83^e6D/Hsp83^9J1 males show defects during spermatogenesis. Excessive numbers of primary spermatocytes are seen in many developing cysts. Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile.

(Yue et al., 1999)

Lethality occurs at embryonic or early larval stages.

(van der Straten et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Hsp83^9J1, Raf¹² has lethal | dominant phenotype

(Dickson et al., 1996)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hsp83^9J1/Hsp83[+] is a suppressor of photoreceptor cell R7 | ectopic phenotype of Raf::tor^12D.hs.sev

(van der Straten et al., 1997)

Hsp83^9J1 is a suppressor of eye phenotype of Raf::tor^12D.sev

(Dickson et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

Presence of Hsp83^13F3 completely eliminates all additional R7 cells in phl::tor^12D.hs.sev mutants. Mutation interacts genetically with the sev mutations; 'sev³¹⁵' and sev^{S11.T:Hsap\MYC}.

(van der Straten et al., 1997)

Dominantly suppresses the rough eye phenotype of phl::tor^12D.sev. Synthetic lethal in combination with phl¹².

(Dickson et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Hsp83^e6D

(van der Straten et al., 1997)

Hsp83^e6A

(van der Straten et al., 1997)