FlyBase Allele Report: Dmel\Hsp83[P582]

General Information

Symbol

Dmel\Hsp83^P582

Species

D. melanogaster

Name

FlyBase ID

FBal0060640

Feature type

allele

Associated gene

Dmel\Hsp83

Associated Insertion(s)

P{}Hsp83^P582

Carried in Construct

Also Known As

P582

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(van der Straten et al., 1997)

loss of function allele

(Atallah et al., 2004)

Mutagen

P-element activity

(van der Straten et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{}Hsp83^P582

Cytology

Description

P-element insertion within the 5' untranslated region, 29bp downstream of the transcription start site.

(van der Straten et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:3,193,045..3,193,065 [+]

(Milton et al., 2003, van der Straten et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity (with Hsp83^13F3)

(Andersen et al., 2012)

abnormal mitotic cell cycle | larval stage

(Lange et al., 2000)

lethal (with Hsp83^6-55)

(Bandura et al., 2013)

lethal | recessive

(Yue et al., 1999, van der Straten et al., 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

Hsp83^13F3/Hsp83^P582 transheterozygous neuroblasts exhibit loss of apical cortical polarity at prophase.

(Andersen et al., 2012)

A Hsp83^P582 background has a highly significant and reproducible effect on total phenotypic variation of scutellar and thoracic bristles. Twice as many Hsp83^P582 mutant flies exhibit abnormal thoracic bristle numbers, with the severity of the thoracic bristle number defects increasing from deviations of at most two thoracic bristles to as many as four. The number of flies with aberrant scutellar bristle numbers increases 4 to 5-fold in Hsp83^P582 mutants.

(Milton et al., 2006)

Hsp83^P582/Hsp83^9J1 mutants develop until larval or early pupae. Larval brains of these mutants exhibit mitotic defects. Polyploid and other aneuploid cells make up about 30% of the mitotic figures seen. About 20% of anaphases are disorganised. The centrosome cycle is also found to be impaired in these cells. There are two classes of defect. Mitotic cells are seen containing a single microtubule organising centre (MTOC) made up of one or two unsegregated centrosomes, leading to the organisation of monopolar mitotic figures. Cells are also seen in which the centrosomes appear to fail in their assembly.

(Lange et al., 2000)

Hsp83⁰⁸⁴⁴⁵/Hsp83^P582 males show defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile.

(Yue et al., 1999)

Lethality occurs at embryonic or early larval stages. Mutation interacts genetically with the sev mutations; 'sev³¹⁵' and sev^{S11.T:Hsap\MYC}.

(van der Straten et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Hsp83^P582/Hsp83[+] is a suppressor of arista | increased number phenotype of obk¹

(Atallah et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

Hsp83⁰⁸⁴⁴⁵

(Yue et al., 1999)

Fails to complement

(Yue et al., 1999)

(Yue et al., 1999)

(Yue et al., 1999)

(Yue et al., 1999)

(Yue et al., 1999)

(Yue et al., 1999)

(Yue et al., 1999)

Rescued by

Hsp83^P582 is rescued by Hsp83^+t7.5

(van der Straten et al., 1997)

Comments

Hsp83^P582 complements Hsp83⁰⁸⁴⁴⁵ for viability and female fertility but not for male fertility.

(Yue et al., 1999)

Lethality can be rescued by Hsp83^+t7.5. Hsp83^+t7.5 also rescues the dominant suppression phenotype.

(van der Straten et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Excision of the P-element reverts the lethal phenotype.

(Yue et al., 1999)

Precise excision of the P-element demonstrates the insertion is responsible for the lethal phenotype.

(van der Straten et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Hsp83⁵⁸²

(Lange et al., 2000)

Hsp83^P582

(Bandura et al., 2013)

Hsp83^P582i

(Milton et al., 2006)

P582

(Bandura et al., 2013, Yue et al., 1999, Rutherford and Lindquist, 1998)

hsp83^p582

(Andersen et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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