FlyBase Allele Report: Dmel\Hsp83[e6D]

General Information

Symbol

Dmel\Hsp83^e6D

Species

D. melanogaster

Name

FlyBase ID

FBal0029645

Feature type

allele

Associated gene

Dmel\Hsp83

Associated Insertion(s)

Carried in Construct

Also Known As

e6D

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(van der Straten et al., 1997)

Mutagen

ethyl methanesulfonate

(van der Straten et al., 1997)

Progenitor genotype

Cytology

Description

Amino acid replacement: E317K. Nucleotide substitution: G949A.

(van der Straten et al., 1997)

Nucleotide substitution: G949A. Amino acid replacement: E317K.

(Cutforth and Rubin, 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:3,195,447..3,195,447 [+]

Nucleotide change:

G3195447A

Reported nucleotide change:

G949A

Amino acid change:

E317K | Hsp83-PA; E317K | Hsp83-PB

Reported amino acid change:

E317K

(Cutforth and Rubin, 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm (with Hsp83⁰⁸⁴⁴⁵)

(Hung et al., 2009)

lethal | recessive

(Yue et al., 1999, Simon et al., 1991)

male sterile (with Hsp83^6-55)

(Bandura et al., 2013)

viable (with Hsp83^9J1)

(Milton et al., 2005)

Phenotype Manifest In

chaeta

(Milton et al., 2005)

embryonic abdominal segment | maternal effect (with Hsp83⁰⁸⁴⁴⁵)

(Song et al., 2007)

Nebenkern

(Yue et al., 1999)

primary spermatocyte cyst

(Yue et al., 1999)

spermatid

(Yue et al., 1999)

spermatocyte

(Yue et al., 1999)

spermatozoon

(Yue et al., 1999)

Detailed Description

Statement

Reference

When challenged with a large phase advance in their environmental temperature cycle in DD, Hsp83^e6D/Hsp83⁰⁸⁴⁴⁵ mutant flies exhibit a significant reduction in the number of 'transition days' associated with the process of phase-resetting daily locomotor activity.

(Goda et al., 2014)

67% of Hsp83⁰⁸⁴⁴⁵/Hsp83^e6D flies show a loss of locomotor behavioral rhythms under free-running conditions (after entrainment in light-dark cycles).

Hsp83⁰⁸⁴⁴⁵/Hsp83^e6D flies show a reduced fraction of flies with rhythmic locomotor activity in light-dark cycles (49% rhythmic) compared to control flies (88%-95% rhythmic).

Hsp83⁰⁸⁴⁴⁵/Hsp83^e6D mutants show highly variable locomotor activity patterns, including fully rhythmic, arrhythmic, and complex behavioral profiles, in contrast to wild-type flies, which show little variation among individuals.

(Hung et al., 2009)

Hsp83^e6D/Hsp83⁰⁸⁴⁴⁵ females are reasonably healthy and produce large numbers of eggs. Very few of the embryos derived from these eggs cellularise. The 1-2% of the embryos that eventually secrete cuticle have on average 3 abdominal segments rather than the normal 8.

(Song et al., 2007)

Hsp83^e6D has no effect on an invariant bristle trait (thoracic and scutellar bristles were analysed). Hsp83^e6D has a significant effect on a variable bristle trait (the sternopleural, orbital, ocellar and vibrissa and carina bristles were analysed); there is a significant difference in trait mean compared to the background strain in which the Hsp83^e6D mutation was induced.

(Milton et al., 2005)

Viable in transheterozygous combination with either Hsp83^9J1; males are sterile and females are weakly fertile. Hsp83⁰⁸⁴⁴⁵/Hsp83^e6D and Hsp83^e6D/Hsp83^9J1 males show defects during spermatogenesis. Excessive numbers of primary spermatocytes are seen in many developing cysts. Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile.

(Yue et al., 1999)

Mutation show strong genetic interaction with sev^{S11.T:Hsap\MYC}, 'sev³⁵¹', phl¹² or phl::tor^12D.hs.sev.

(van der Straten et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsp83^e6D/Hsp83[+] is a suppressor of visible phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

Phenotype Manifest In

Enhancer of

Statement

Reference

Hsp83^e6D/Hsp83[+] is an enhancer | maternal effect of embryonic abdominal segment | maternal effect phenotype of nanos^ΔBX, nanos^BN

(Song et al., 2007)

Hsp83^e6D/Hsp83[+] is an enhancer of arista | increased number phenotype of obk¹

(Atallah et al., 2004)

Suppressor of

Statement

Reference

Hsp83^e6D is a suppressor of wing vein phenotype of Egfr^E1

(Salamanca et al., 2011)

Hsp83^e6D is a suppressor of eye phenotype of Raf⁹⁸

(Salamanca et al., 2011)

Hsp83^e6D/Hsp83[+] is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

Hsp83^e6D is a suppressor of embryonic/first instar larval cuticle | maternal effect phenotype of tor^12D

(Li and Li, 2003)

Other

Statement

Reference

Hsp83^e6D, Kr^If-1 has eye phenotype

(Sollars et al., 2003)

Hsp83^e6D/Hsp83[+], Kr^If-1 has eye phenotype

(Sollars et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

A Hsp83^e6D background suppresses the abnormal wing vein morphology found in Egfr^E1 heterozygous flies.

The presence of Hsp83^e6D reduces the rough eye morphology seen in Raf⁹⁸ flies.

(Salamanca et al., 2011)

The mutant abdominal segmentation phenotype seen in females carrying nos^ΔBX in a nos^BN/nos^BN background is enhanced (abdominal segmentation is reduced) if the females also carry one copy of Hsp83^e6D.

(Song et al., 2007)

13+/-4% of Kr^If-1/+ flies born to Hsp83^e6D/+ mothers have outgrowths with ectopic vibrissae protruding from the ventral region of the eye, compared to less than 0.1% of Kr^If-1/+ flies born to isogenised wild-type mothers.

(Sollars et al., 2003)

Enhances the sev^B4 phenotype: disrupts sev^B4 protein signalling.

(Simon et al., 1991)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Hsp83^9J1

(van der Straten et al., 1997)

Partially complements

Hsp83⁰⁸⁴⁴⁵

(Yue et al., 1999)

Fails to complement

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5696

w^*; Hsp83^e6D/TM3, Sb¹

Kyoto

108373

w^*; Hsp83^e6D/TM6C, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

E(sev)3A^e6D

E317K

(Yue et al., 1999)

Hsap83^E317K

(Kimura et al., 1997)

Hsp83^E317K

(Song et al., 2007)

Hsp83^e6D

(Bandura et al., 2013, Oishi et al., 2006)

Hsp90^e6D

(Goda et al., 2014)

e6D

(Bandura et al., 2013, Rutherford and Lindquist, 1998)

hsp83^e6D

(Hung et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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