FlyBase Allele Report: Dmel\Hsp83[13F3]

General Information

Symbol

Dmel\Hsp83^13F3

Species

D. melanogaster

Name

FlyBase ID

FBal0049163

Feature type

allele

Associated gene

Dmel\Hsp83

Associated Insertion(s)

Carried in Construct

Also Known As

13F3

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(van der Straten et al., 1997, Dickson et al., 1996)

Mutagen

ethyl methanesulfonate

(van der Straten et al., 1997, Dickson et al., 1996)

Progenitor genotype

Cytology

Description

Point mutation. Amino acid replacement: R48C. Nucleotide substitution: C142T.

(van der Straten et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:3,194,640..3,194,640 [+]

Nucleotide change:

C3194640T

Reported nucleotide change:

C142T

Amino acid change:

R48C | Hsp83-PA; R48C | Hsp83-PB

Reported amino acid change:

R48C

(van der Straten et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity (with Hsp83^P582)

(Andersen et al., 2012)

lethal | recessive

(Yue et al., 1999, Dickson et al., 1996)

lethal - all die before end of larval stage | recessive

(van der Straten et al., 1997)

partially lethal (with Hsp83^6-55)

(Bandura et al., 2013)

some die during larval stage | recessive

(van der Straten et al., 1997)

viable (with Hsp83^e1D)

(Milton et al., 2005)

viable (with Hsp83^e3A)

(Milton et al., 2005)

Phenotype Manifest In

Nebenkern

(Yue et al., 1999)

neuroblast (with Hsp83^P582)

(Andersen et al., 2012)

spermatid

(Yue et al., 1999)

spermatocyte

(Yue et al., 1999)

spermatozoon

(Yue et al., 1999)

Detailed Description

Statement

Reference

Hsp83^13F3/Hsp83^P582 transheterozygous neuroblasts exhibit loss of apical cortical polarity at prophase.

(Andersen et al., 2012)

Hsp83^13F3 has no effect on an invariant bristle trait (thoracic and scutellar bristles were analysed). Hsp83^13F3 has no significant effect on the trait mean of a variable bristle trait (the sternopleural, orbital, ocellar and vibrissa and carina bristles were analysed) compared to the background strain in which the Hsp83^13F3 mutation was induced.

(Milton et al., 2005)

Viable in transheterozygous combination with either Hsp83^e1D or Hsp83^e3A; males are sterile and females are weakly fertile. Viable in transheterozygous combination with Hsp83^e6A; males and females are sterile. Hsp83^e1D/Hsp83^13F3 and Hsp83^13F3/Hsp83^e3A males show defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile.

(Yue et al., 1999)

Mutation interacts genetically with the sev mutations; 'sev³¹⁵' and sev^{S11.T:Hsap\MYC}.

(van der Straten et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Hsp83[+]/Hsp83^13F3 is a suppressor of photoreceptor cell R7 | ectopic phenotype of Raf::tor^12D.hs.sev

(van der Straten et al., 1997)

Hsp83^13F3 is a suppressor of eye phenotype of Raf::tor^12D.sev

(Dickson et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

Presence of Hsp83^13F3 completely eliminates all additional R7 cells in phl::tor^12D.hs.sev mutants.

(van der Straten et al., 1997)

Dominantly suppresses the rough eye phenotype of phl::tor^12D.sev.

(Dickson et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Hsp83^e6A

(van der Straten et al., 1997)

Hsp83^e3A

(van der Straten et al., 1997)

Hsp83^e1D

(van der Straten et al., 1997)

Fails to complement

(Yue et al., 1999)

(Yue et al., 1999)

(Yue et al., 1999)

(Yue et al., 1999)

(Yue et al., 1999)

(van der Straten et al., 1997)

Hsp83^e4A

(van der Straten et al., 1997)

Hsp83^9J1

(van der Straten et al., 1997)

Rescued by

Hsp83^13F3 is rescued by Hsp83^+t7.5

(van der Straten et al., 1997)

Comments

Lethality can be rescued by Hsp83^+t7.5. Hsp83^+t7.5 also rescues the dominant suppression phenotype. Transheterozygotes with Hsp83^e6D or Hsp83^e4A are viable and result in male sterility with no other signs of abnormal development.

(van der Straten et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Hsp83^13F3 and Hsp83^19F2 are independent isolates of the same point mutation.

(Rutherford and Lindquist, 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

13F3

(Bandura et al., 2013, Rutherford and Lindquist, 1998)

Hsap83^R48C

(Kimura et al., 1997)

Hsp83^13F3

(Bandura et al., 2013)

R48C

(Yue et al., 1999)

Su(Raf)3A^13F3

hsp83^13F3

(Andersen et al., 2012, van der Straten et al., 1997)

Name Synonyms

Secondary FlyBase IDs

References (9)

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