FlyBase Allele Report: Dmel\Ser[RX106]

General Information

Symbol

Dmel\Ser^RX106

Species

D. melanogaster

Name

FlyBase ID

FBal0030221

Feature type

allele

Associated gene

Dmel\Ser

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Couso et al., 1995, Speicher et al., 1994, de Celis et al., 1993)

loss of function allele

(Micchelli et al., 1997, de Celis et al., 1996)

Mutagen

X ray

(Speicher et al., 1994, Thomas et al., 1991)

Progenitor genotype

Ser¹

(Speicher et al., 1994, Thomas et al., 1991)

Cytology

Polytene chromosomes normal.

(Speicher et al., 1994)

Description

Exon 6 and part of exon 7 are deleted.

(Baker and Yu, 1997)

Internal deletion of 9kb of the transcribed region.

(Thomas et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity | embryonic stage

(Wiellette and McGinnis, 1999)

lethal - all die before end of larval stage | recessive

(Speicher et al., 1994)

some die during larval stage | recessive

(Speicher et al., 1994)

visible | recessive

(Bishop et al., 1999, Klein and Martinez Arias, 1998)

visible | somatic clone

(de Celis et al., 1996)

Phenotype Manifest In

denticle row 3 & abdominal 2 ventral denticle belt

(Wiellette and McGinnis, 1999)

denticle row 3 & abdominal 3 ventral denticle belt

(Wiellette and McGinnis, 1999)

denticle row 3 & abdominal 4 ventral denticle belt

(Wiellette and McGinnis, 1999)

denticle row 3 & abdominal 5 ventral denticle belt

(Wiellette and McGinnis, 1999)

denticle row 3 & abdominal 6 ventral denticle belt

(Wiellette and McGinnis, 1999)

denticle row 3 & abdominal 7 ventral denticle belt

(Wiellette and McGinnis, 1999)

denticle row 3 & abdominal 8 ventral denticle belt

(Wiellette and McGinnis, 1999)

denticle row 4 & abdominal 2 ventral denticle belt

(Wiellette and McGinnis, 1999)

denticle row 4 & abdominal 3 ventral denticle belt

(Wiellette and McGinnis, 1999)

denticle row 4 & abdominal 4 ventral denticle belt

(Wiellette and McGinnis, 1999)

denticle row 4 & abdominal 5 ventral denticle belt

(Wiellette and McGinnis, 1999)

denticle row 4 & abdominal 6 ventral denticle belt

(Wiellette and McGinnis, 1999)

denticle row 4 & abdominal 7 ventral denticle belt

(Wiellette and McGinnis, 1999)

denticle row 4 & abdominal 8 ventral denticle belt

(Wiellette and McGinnis, 1999)

dorsal bridge

(Wiellette and McGinnis, 1999)

dorsal pouch

(Wiellette and McGinnis, 1999)

embryonic/larval anterior spiracle

(Speicher et al., 1994)

embryonic/larval digestive system | embryonic stage

(Wiellette and McGinnis, 1999)

embryonic/larval proventriculus | embryonic stage

(Wiellette and McGinnis, 1999)

hub cell | first instar larval stage

(Okegbe and DiNardo, 2011)

lateral process of cephalopharyngeal skeleton

(Wiellette and McGinnis, 1999)

leg & joint

(Bishop et al., 1999)

leg joint

(Rauskolb and Irvine, 1999)

mouth hook

(Wiellette and McGinnis, 1999, Speicher et al., 1994)

papilla of larval anterior spiracle

(Speicher et al., 1994)

tarsal segment 1 & joint

(Bishop et al., 1999)

tarsal segment 2 & joint

(Bishop et al., 1999)

tarsal segment 3 & joint

(Bishop et al., 1999)

tarsal segment 4 & joint

(Bishop et al., 1999)

tarsal segment 5 & joint

(Bishop et al., 1999)

wing

(Klein and Martinez Arias, 1998, Klein and Martinez Arias, 1998, Speicher et al., 1994)

wing blade dorsal compartment | somatic clone

(de Celis et al., 1996)

wing disc

(Speicher et al., 1994)

wing disc (with Ser^CS94)

(Klein, 2003, Klein and Martinez Arias, 1999)

Detailed Description

Statement

Reference

Mutant male first instar larvae raised at 25[o]C show a reduction in the number of hub cells in the gonad compared to controls.

(Okegbe and DiNardo, 2011)

Ser^RX106/+ markedly enhances the loss of eye tissue caused by expression of mir-8^SC1 under the control of Scer\GAL4^ey.PH.

(Vallejo et al., 2011)

Homozygous intestinal stem cell (ISC) clones in the adult midgut show normal development.

(Ohlstein and Spradling, 2007)

The second mitotic wave occurs normally in clones of Ser^RX106 mutant cells in a Minute/+ background in the eye disc.

(Baonza and Freeman, 2005)

Ser^RX106 mutants display no lateral inhibition defects in sensory organ precursors in third instar nota.

(Pitsouli and Delidakis, 2005)

In Ser^RX106 homozygous embryos the distal ends of the salivary gland ducts are enlarged/splayed in the region where they contact the secretory cells.

(Haberman et al., 2003)

Mutant embryos form normal looking hindgut boundary cells.

(Iwaki and Lengyel, 2002)

Pupal lethal when heterozygous with the MBT chromosome.

(Riede, 2001)

Ser^RX106/Df(3R)D605 animals do not survive but a few mutant flies are formed inside the pupal cases. These flies have leg deformities, lacking the joints between all segments, although sometimes a remnant of a constriction can be seen. No other leg area is affected and the segment boundaries of the leg are still present as shown by the apical bristles in the tarsi and tibia.

(Bishop et al., 1999)

Embryos do not show any abnormality in the overall pattern of the tracheal system.

(Ikeya and Hayashi, 1999)

Ser^RX106/Ser^CS94 animals show loss of wing disc tissue.

(Klein and Martinez Arias, 1999)

Homozygous Dl^RevF10 Ser^RX106 double mutant clones in the leg result in a failure to form joints. In most cases, the failure to form joints is an autonomous property of the mutant cells in the clone.

(Rauskolb and Irvine, 1999)

Approximately half of homozygous larvae show complete fusion of denticle rows 3 and 4 in abdominal segments A2 to A8. The denticles in the fused row have no regular polarity and about half the larvae develop small denticles in this row. Ubiquitous expression of Ser^Scer\UAS.cGa under the control of Scer\GAL4^arm.PS in a Ser^RX106/Df(3R)Bd background results in severe morphological defects; the mouth hooks develop additional sclerotic material at the middle and base and about a quarter of the embryos show duplication of the mouth hook tips. Extra sclerotic material also develops in the dorsal pouch. The phenotype varies from an extended and fragmented dorsal bridge to extreme sclerotisation of the dorsal pouch and shortening of the lateralgraten. Excessive sclerotisation is seen in the proventriculus and gut. Homozygous and wild-type embryos show comparable patterns of BrdU incorporation during the final round of ventral epidermal cell division.

(Wiellette and McGinnis, 1999)

Ser^CS94/Ser^RX106 flies develop a very small wing and hinge.

(Klein and Martinez Arias, 1998)

Ser^CS94/Ser^RX106 flies have a small marginless wing blade.

(Klein and Martinez Arias, 1998)

Neural differentiation does not occur in Dl^RevF10 Ser^RX106 double mutant clones in the eye disc, except near the clone margins. Where neural differentiation occurs near the clone margins, excessive numbers of R8 cells are seen. This phenotype resembles that seen for clones homozygous for Dl^- alone.

(Ligoxygakis et al., 1998)

Homozygous clones in the adult scutum have normal bristle structures. Homozygous clones induced in the sensory organ lineage have a similar phenotype to homozygous Ser^RX82 clones induced in the sensory organ lineage.

(Zeng et al., 1998)

Loss of Ser function has no discernible effect on R8 differentiation in the eye disc (clonal analysis).

(Baker and Yu, 1997)

Homozygous clones abutting the wing margin in the dorsal wing compartment or induced before dorso-ventral lineage segregation cause extensive wing scalloping.

(de Celis et al., 1996)

Df(3R)D605/Ser^RX106 flies have wings reduced to stumps that have no margin.

(Couso et al., 1995)

Anterior spiracles do not develop knob-like structure typical of first and second larval instar, nor the retractable fingerlike structures characteristic of third instar larvae. The tracheal trunk terminates without opening to the exterior anteriorly, but the posterior spiracles look normal. Mouth hooks always exhibit features characteristic of first instar larvae. Some imaginal discs are smaller than wild type, particularly the dorsal mesothoracic discs. The size of the humeral disc appears normal. Pharate adult escapers are lacking wings and halteres, genital apparatus and anal plates are small or missing, tarsal segments are fused and eyes are rough and reduced. Clones induced in adults are normal in head, thorax legs and abdomen, but produce nicking and deletions in the wing.

(Speicher et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Ser[+]/Ser^RX106 is an enhancer of visible phenotype of Scer\GAL4^ey.PH, mir-8^SC1

(Vallejo et al., 2011)

Ser[+]/Ser^RX106 is an enhancer of visible phenotype of fj^N7

(Buckles et al., 2001)

Suppressor of

Statement

Reference

Ser[+]/Ser^RX106 is a suppressor | partially of visible | heat sensitive phenotype of Dcr-2^UAS.cDa, Eogt^GD5084, Scer\GAL4^en.PU

(Müller et al., 2013)

Ser[+]/Ser^RX106 is a suppressor | partially of visible phenotype of mir-8^Δ2/Df(2R)mir-8^Δ3

(Vallejo et al., 2011)

Ser[+]/Ser^RX106 is a suppressor of abnormal size phenotype of mir-8^Δ2/Df(2R)mir-8^Δ3

(Vallejo et al., 2011)

Other

Statement

Reference

Delta^RevF10, Ser^RX106 has abnormal neuroanatomy | embryonic stage phenotype

(Monastirioti et al., 2010)

Delta^RevF10, Ser^RX106 has hyperplasia | embryonic stage phenotype

(Monastirioti et al., 2010)

Ser^RX106, srn⁸⁸ has lethal | pupal stage phenotype

(Riede, 2001)

Ser^RX106, tld⁶ has lethal | pupal stage phenotype

(Riede, 2001)

Df(3R)mbt^P, Ser^RX106 has lethal | pupal stage phenotype

(Riede, 2001)

Ser^RX106, efe⁷⁹ has lethal | pupal stage phenotype

(Riede, 2001)

Dfd¹³/Dfd³, Ser^RX106 has partially lethal phenotype

(Wiellette and McGinnis, 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

Delta^RevF10, Ser^RX106 has external sensory organ precursor cell | somatic clone phenotype, suppressible by Delta^{ΔICD2.UAS.Tag:V5,Tag:polyHis}/Scer\GAL4^{VP16.mat.αTub67C}

(Daskalaki et al., 2011)

Ser^CS94/Ser^RX106 has wing disc phenotype, suppressible by l(2)gd1^d7

(Klein, 2003)

Ser^CS94/Ser^RX106 has wing disc phenotype, suppressible by Delta^UAS.cHa/Scer\GAL4^dpp.blk1

(Klein and Martinez Arias, 1999)

Ser^CS94/Ser^RX106 has wing phenotype, suppressible by Scer\GAL4^dpp.blk1/vg^UAS.cKa

(Klein and Martinez Arias, 1998)

NOT suppressed by

Statement

Reference

Delta^RevF10, Ser^RX106 has external sensory organ phenotype, non-suppressible | somatic clone by AP-1μ^SHE-11

(Benhra et al., 2011)

Delta^RevF10, Ser^RX106 has external sensory organ precursor cell | somatic clone phenotype, non-suppressible by Delta^{ΔICD1.UAS.Tag:V5,Tag:polyHis}/Scer\GAL4^{VP16.mat.αTub67C}

(Daskalaki et al., 2011)

Delta^RevF10, Ser^RX106 has external sensory organ precursor cell | somatic clone phenotype, non-suppressible by Delta^{ΔICD1.ΔICD2.UAS.Tag:V5,Tag:polyHis}/Scer\GAL4^{VP16.mat.αTub67C}

(Daskalaki et al., 2011)

Delta^RevF10, Ser^RX106 has external sensory organ precursor cell | somatic clone phenotype, non-suppressible by Scer\GAL4^{VP16.mat.αTub67C}/Delta^{ΔC.UAS.Tag:V5,Tag:polyHis}

(Daskalaki et al., 2011)

Delta^RevF10, Ser^RX106 has external sensory organ precursor cell | somatic clone phenotype, non-suppressible by Delta^{UAS.LDL+,Tag:MYC}/Scer\GAL4^{VP16.mat.αTub67C}

(Daskalaki et al., 2011)

Enhancer of

Statement

Reference

Ser[+]/Ser^RX106 is an enhancer of eye phenotype of Scer\GAL4^ey.PH, mir-8^SC1

(Vallejo et al., 2011)

Ser^RX106 is an enhancer of sensory mother cell | third instar larval stage | ectopic | somatic clone phenotype of Delta^RevF10

(Pitsouli and Delidakis, 2005)

Ser^RX106 is an enhancer of sensory mother cell | ectopic | somatic clone phenotype of neur¹

(Pitsouli and Delidakis, 2005)

Ser[+]/Ser^RX106 is an enhancer of leg joint phenotype of fj^N7

(Buckles et al., 2001)

NOT Enhancer of

Statement

Reference

Ser[+]/Ser^RX106 is a non-enhancer of eye phenotype of Scer\GAL4^ey.PH, fng^UAS.cKa

(Dominguez et al., 2004)

Suppressor of

Statement

Reference

Ser[+]/Ser^RX106 is a suppressor | partially of wing blade posterior compartment | heat sensitive phenotype of Dcr-2^UAS.cDa, Eogt^GD5084, Scer\GAL4^en.PU

(Müller et al., 2013)

Ser^RX106/Delta^RevF10 is a suppressor of tormogen cell | increased number | somatic clone phenotype of AP-1μ^SHE-11

(Benhra et al., 2011)

Ser[+]/Ser^RX106 is a suppressor of wing phenotype of mir-8^Δ2/Df(2R)mir-8^Δ3

(Vallejo et al., 2011)

Ser[+]/Ser^RX106 is a suppressor | partially of wing vein | ectopic phenotype of mir-8^Δ2/Df(2R)mir-8^Δ3

(Vallejo et al., 2011)

NOT Suppressor of

Statement

Reference

Ser^CS94/Ser^RX106 is a non-suppressor of anterior dorsocentral bristle phenotype of Scer\GAL4^klu-G410, fng^UAS.cKa

(Klein and Martinez Arias, 1998)

Other

Statement

Reference

Delta^RevF10, Ser^RX106 has external sensory organ | somatic clone phenotype

(Benhra et al., 2011)

Delta^RevF10, Ser^RX106 has external sensory organ precursor cell | somatic clone phenotype

(Daskalaki et al., 2011)

Delta^RevF10, Ser^RX106 has presumptive embryonic/larval central nervous system phenotype

(Monastirioti et al., 2010)

Delta^RevF10, Ser^RX106 has embryonic/larval neuron | increased number phenotype

(Monastirioti et al., 2010)

Delta^RevF10, Ser^RX106 has wing disc | somatic clone phenotype

(Rauskolb et al., 1999)

Scer\GAL4^dpp.blk1, Ser^RX106, vg^UAS.cKa has wing phenotype

(Klein and Martinez Arias, 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The wing blistering phenotype seen in the posterior compartment of wings in flies expressing Eogt^GD5084 under the control of Scer\GAL4^en.PU in the presence of Dcr-2^Scer\UAS.cDa is dominantly partially suppressed if the flies are also heterozygous for Ser^RX106.

(Müller et al., 2013)

Dl^RevF10, Ser^RX106 double mutant clones in the adult thorax exhibit external sensory cell loss and an excess of neurons.

A Dl^RevF10 Ser^RX106 double mutant background suppresses the extra socket cell phenotype seen in AP-47^SHE-11 thorax clones, instead producing the external sensory cell loss and excess neurons seen in Dl^RevF10 Ser^RX106 double mutants.

(Benhra et al., 2011)

Wing disc clones mutant for Dl^RevF10 and Ser^RX106 give rise to clustered supernumerary sensory organ precursors (SOP), as all cells within the proneural field adopt the SOP fate. These are often spaced apart from each other, suggesting that a low level of lateral inhibition is still taking place.

Expression of Dl^{ΔICD2.Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of the ubiquitous driver Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} restores N signalling in Dl^RevF10 Ser^RX106 mutant clones, leading to individualised sensory organ precursors. These are often spaced apart from each other, suggesting that a low level of lateral inhibition is still taking place.

Expression of Dl^{ΔICD1.Scer\UAS.T:SV5\V5,T:Zzzz\His6} or Dl^{ΔICD1.ΔICD2.Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of the ubiquitous driver Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} fails to restore lateral inhibition by N signalling in Dl^RevF10 Ser^RX106 mutant clones. These are often spaced apart from each other, suggesting that a low level of lateral inhibition is still taking place.

Expression of Dl^{ΔC.Scer\UAS.T:SV5\V5,T:Zzzz\His6} or Dl^{K742R.Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of the ubiquitous driver Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} fails to restore lateral inhibition N signalling in Dl^RevF10 Ser^RX106 mutant clones.

Expression of Dl^{Scer\UAS.T:Hsap\LDLR-int,T:Hsap\MYC} under the control of the ubiquitous driver Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} does not rescue N-mediated lateral inhibition in Dl^RevF10 Ser^RX106 mutant clones, as a large number of adjacent sensory organ precursors are reproducibly detected.

(Daskalaki et al., 2011)

Ser^RX106/+ fully rescues the defects in wing size and partially suppresses the extra wing vein phenotype caused by mir-8^Δ2/Df(2R)mir-8^Δ3.

(Vallejo et al., 2011)

Dl^RevF10 Ser^RX106 third instar nota clones exhibit at least eight sensory organ precursors (SOPs) in approximately 70.5% of SOP positions scored. Approximately 21.5% of SOP positions display between four and eight ectopic SOPs, while approximately 6% exhibit between one and four SOPs, with only 2% exhibiting one SOP, as in wild-type. Addition of the Dl^Scer\UAS.cLa transgene, under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} partially rescues the ectopic SOP phenotype, with approximately 8.5% of SOP positions exhibiting between four and eight SOPs. Addition of the Ser^Scer\UAS.cGa transgene, under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, partially rescues the ectopic SOP phenotype, with approximately 26% of SOP positions exhibiting between one and four SOPs. neur¹ Ser^RX106 third instar nota clones exhibit at least eight sensory organ precursors (SOPs) in approximately 33.5% of SOP positions scored. Approximately 47% of SOP positions display between four and eight ectopic SOPs, while approximately 19.5% exhibit between one and four SOPs. No SOP positions appeared wild-type. Addition of the Ser^Scer\UAS.cGa transgene, to Dl^RevF10 Ser^RX106 double mutant third instar nota clones, under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, partially rescues the ectopic SOP phenotype, with approximately 26% of SOP positions exhibiting between one and four SOPs.

(Pitsouli and Delidakis, 2005)

The small eye phenotype of flies in which fng^Scer\UAS.cKa is expressed under the control of Scer\GAL4^ey.PH is not enhanced in Ser^RX106 heterozygotes.

(Dominguez et al., 2004)

The loss of wing disc tissue seen in Ser^RX106/Ser^CS94 animals is rescued by Dl^Scer\UAS.cHa expressed under the control of Scer\GAL4^dpp.blk1.

(Klein and Martinez Arias, 1999)

Dl^RevF10,Ser^RX106 double mutant somatic clones in wing discs fail to respect the dorsal ventral boundary.

(Rauskolb et al., 1999)

Enhances the Dfd¹³/Dfd³ phenotype; reduces the survival of Dfd¹³/Dfd³ hypomorphs. All of the denticles in rho⁶ Ser^RX106 double mutant embryos are similar to each other and resemble a composite of type 5 and 2 denticles. The denticles are disorganised and separate rows are not distinguishable.

(Wiellette and McGinnis, 1999)

Scer\GAL4^dpp.blk1-mediated expression of vg^Scer\UAS.cKa substantially rescues the wing blade.

(Klein and Martinez Arias, 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99583

mwh¹ red¹ e¹ Ser^RX106/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

See Thomas et al., 1991, Development 111:749--761 .

(de Celis et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Ser¹⁰⁶

(Okegbe and DiNardo, 2011)

Ser^RX106

(Kalodimou et al., 2023, Zacharioudaki et al., 2016, LeBon et al., 2014, Müller et al., 2013, Xie et al., 2012, Benhra et al., 2011, Daskalaki et al., 2011, Okegbe and DiNardo, 2011, Vallejo et al., 2011, Monastirioti et al., 2010, Wang and Huang, 2009, Grammont, 2007, Ohlstein and Spradling, 2007, Sasaki et al., 2007, Childress et al., 2006, Childress et al., 2006, Glittenberg et al., 2006, Baonza and Freeman, 2005, Brodu et al., 2004)

Ser^Rx106

(Martins et al., 2021, Walters et al., 2005, Riede, 2001, Klein and Martinez Arias, 1998, Klein and Martinez Arias, 1998, Zeng et al., 1998, Jonsson and Knust, 1996, Speicher et al., 1994)

Ser^rx106

(Beehler-Evans and Micchelli, 2015, Poulton et al., 2011, Wiellette and McGinnis, 1999)

Serrate^RX106

(Ward et al., 2006)

Serrate^Rx106

(Ohlstein and Spradling, 2007)

ser^RX106

(Alexandre et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (57)

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