FlyBase Allele Report: Dmel\l(2)gd1[d7]

General Information

Symbol

Dmel\l(2)gd1^d7

Species

D. melanogaster

Name

FlyBase ID

FBal0043607

Feature type

allele

Associated gene

Dmel\l(2)gd1

Associated Insertion(s)

Carried in Construct

Also Known As

lgd^d7

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

PM hybrid dysgenesis

Nature of the Allele

Allele class

Mutagen

PM hybrid dysgenesis

(Buratovich and Bryant, 1995)

Progenitor genotype

Cytology

Description

Carries a single nucleotide deletion that results in a new translational frame and a premature stop codon after the addition of 22 new amino acids.

(Childress et al., 2006)

A 2 bp deletion at amino acid position 215 which results in a frame shift and a translational stop 14 amino acids downstream, leading to a truncated protein containing only the first DM14 domain.

(Jaekel and Klein, 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:11,107,795..11,107,796 [+]

Comment:

A two base pair deletion within amino acid 215 that results in a frameshift and translation termination 14aa downstream. The two deleted bases within the codon were not specified.

(Jaekel and Klein, 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg chamber | germline clone

(Matias et al., 2015)

endosome | somatic clone

(Schneider et al., 2013)

female germline cell | germline clone

(Matias et al., 2015)

female germline stem cell | germline clone

(Matias et al., 2015)

follicle cell | somatic clone

(Schneider et al., 2013)

imaginal disc

(Buratovich and Bryant, 1997)

late endosome

(Schneider et al., 2013)

late endosome, with Scer\GAL4^hh-Gal4, l(2)gd1^NIG.4713R

(Schneider et al., 2013)

late endosome | somatic clone

(Jaekel and Klein, 2006)

leg joint | ectopic | pharate adult stage (with l(2)gd1^SH0495)

(Jaekel and Klein, 2006)

macrochaeta | pharate adult stage (with l(2)gd1^SH0495)

(Jaekel and Klein, 2006)

mesothoracic tergum | pharate adult stage (with l(2)gd1^SH0495)

(Jaekel and Klein, 2006)

mesothoracic tergum | somatic clone

(Jaekel and Klein, 2006)

metathoracic leg disc

(Buratovich and Bryant, 1995)

microchaeta | pharate adult stage (with l(2)gd1^SH0495)

(Jaekel and Klein, 2006)

peripodial epithelium | posterior, with Scer\GAL4^hh-Gal4, l(2)gd1^NIG.4713R

(Schneider et al., 2013)

sensory mother cell | somatic clone

(Klein, 2003)

stalk follicle cell | somatic clone

(Schneider et al., 2013)

tarsal segment | pharate adult stage (with l(2)gd1^SH0495)

(Jaekel and Klein, 2006)

tormogen cell | pharate adult stage | increased number (with l(2)gd1^SH0495)

(Jaekel and Klein, 2006)

wing disc posterior compartment, with Scer\GAL4^hh-Gal4, l(2)gd1^NIG.4713R

(Schneider et al., 2013)

wing vein | somatic clone

(Klein, 2003)

wing vein L2 | pharate adult stage (with l(2)gd1^SH0495)

(Jaekel and Klein, 2006)

wing vein L4 | pharate adult stage (with l(2)gd1^SH0495)

(Jaekel and Klein, 2006)

wing vein L5 | pharate adult stage (with l(2)gd1^SH0495)

(Jaekel and Klein, 2006)

Detailed Description

Statement

Reference

21% of homozygous egg chambers contain 32 cells. 69% of homozygous germaria contain 'stem cysts' in which the female germline stem cells are interconnected to daughter cells.

(Matias et al., 2015)

l(2)gd1^d7 wing discs contain enlarged maturing endosomes compared to wild type.

l(2)gd1^d7 mutants do not exhibit autophagy or eye disc differentiation defects.

Scer\GAL4^hh-Gal4-mediated expression of l(2)gd1^NIG.4713R (in the presence of Dcr-2^Scer\UAS.cDa) in a l(2)gd1^d7/+ background results in posterior compartment peripodial cells of the wing disc adopting a columnar shape and forming a continuation of the lateral part of the disc epithelium - i.e. a transformation of peripodial into disc cells. TEM analysis shows that posterior compartment cells contain a class of endosomes that are enlarged compared to normal cells, though normal sized multi-vesicular bodies are also present. Lysosomes are of normal size.

Oocyte follicle cells mutant for l(2)gd1^d7 precociously go through the mitotic-to-endocycle switch, as evidenced by the increased size of their nuclei compared to controls. The stalk that connects the egg chambers is elongated compared to controls. Endosomes are enlarged.

(Schneider et al., 2013)

l(2)gd1^SH0495/l(2)gd1^d7 pharates exhibit: loss of notal bristles, duplications/quadruplications of notal socket cells at the expense of other cell fates of this organ, foreshortening (vein 4) or loss (veins 2 and 5) of vein tissue, ectopic formation of leg joints and a reduction of the tarsal region.

Wing pouch clones show increased cell death.

Adult flies bearing l(2)gd1^d7 clones sometimes show tumorous growths in nota.

Cells of l(2)gd1^d7 wing disc clones form/accumulate late endosomes.

(Jaekel and Klein, 2006)

If l(2)gd1^d7 somatic clones are made in the wing disc during the first instar stage, they are rarely found in wing pouches of late third instar larvae, occasionally leaving scars. However clones are frequently recovered outside the pouch. If induced later some mutant clones are found in the wing pouch though many more twin spot clones are seen than mutant ones. Mutant clones are rounded in shape. Wings that develop from these discs show wing vein disruption. In l(2)gd1 mutant discs, some proneural clusters are formed, but the sensory organ precursors do not form.

(Klein, 2003)

Overgrowing imaginal discs show pattern duplications. Phenotype is more severe than for l(2)gd1¹.

(Buratovich and Bryant, 1997)

Homozygotes display imaginal disc overgrowth and frequent disc duplication.

(Buratovich and Bryant, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

l(2)gd1^d7 has decreased cell number | larval stage | somatic clone phenotype, enhanceable by N^Ax-M1

(Klein, 2003)

Suppressed by

Statement

Reference

l(2)gd1^d7 has increased cell death | somatic clone phenotype, suppressible by Scer\GAL4^Tub.PU/BacA\p35^UAS.cHa

(Jaekel and Klein, 2006)

l(2)gd1^d7 has decreased cell number | somatic clone | larval stage phenotype, suppressible by Psn^C1

(Klein, 2003)

NOT suppressed by

Statement

Reference

l(2)gd1^d7 has increased cell death | somatic clone phenotype, non-suppressible by Su(H)⁸

(Jaekel and Klein, 2006)

Enhancer of

Statement

Reference

l(2)gd1[+]/l(2)gd1^d7 is an enhancer of abnormal cytokinesis | dominant phenotype of shrb^G5

(Matias et al., 2015)

Other

Statement

Reference

l(2)gd1^d7/l(2)gd1^SH0495, shrb[+]/shrb⁴ has lethal | third instar larval stage phenotype

(Troost et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

l(2)gd1^d7 has phenotype, enhanceable by N^UAS.cUa/Scer\GAL4^hh-Gal4

(Schneider et al., 2013)

l(2)gd1^d7 has phenotype, enhanceable by N^{ΔEGF1-36.LV.UAS}/Scer\GAL4^hh-Gal4

(Schneider et al., 2013)

NOT Enhanced by

Statement

Reference

l(2)gd1^d7 has phenotype, non-enhanceable by Hsap\LAMP1^UAS.EGFP/Scer\GAL4^hh-Gal4

(Schneider et al., 2013)

Suppressed by

Statement

Reference

l(2)gd1^d7 has phenotype, suppressible by tkv^UAS.GFP/Scer\GAL4^hh-Gal4

(Schneider et al., 2013)

l(2)gd1^d7 has phenotype, suppressible by Scer\GAL4^hh-Gal4/N^{intra.CD8.UAS.EGFP}

(Schneider et al., 2013)

l(2)gd1^d7 has phenotype, suppressible by Scer\GAL4^hh-Gal4/T48^UAS.GFP

(Schneider et al., 2013)

l(2)gd1^d7 has phenotype, suppressible by Hrs^D28

(Schneider et al., 2013)

l(2)gd1^d7 has phenotype, suppressible by aph-1^D35

(Schneider et al., 2013)

l(2)gd1^d7 has phenotype, suppressible by aph-1[+]/aph-1^D35

(Schneider et al., 2013)

l(2)gd1^d7 has phenotype, suppressible by Vha68-1^GD7717/Scer\GAL4^hh-Gal4

(Schneider et al., 2013)

l(2)gd1^d7 has phenotype, suppressible by Scer\GAL4^hh-Gal4/N^BC.lexA.UAS

(Schneider et al., 2013)

l(2)gd1^d7 has phenotype, suppressible by Scer\GAL4^hh-Gal4/Rab7^GD11800

(Schneider et al., 2013)

l(2)gd1^d7 has phenotype, suppressible by car¹/car¹

(Schneider et al., 2013)

l(2)gd1^d7 has phenotype, suppressible by Vps39^GD12152/Scer\GAL4^hh-Gal4

(Schneider et al., 2013)

l(2)gd1^d7/l(2)gd1^SH0495 has leg joint | ectopic phenotype, suppressible | partially by Psn^C1/Psn[+]

(Jaekel and Klein, 2006)

l(2)gd1^d7/l(2)gd1^SH0495 has tarsal segment phenotype, suppressible | partially by Psn^C1/Psn[+]

(Jaekel and Klein, 2006)

l(2)gd1^d7 has wing disc phenotype, suppressible by Psn^C1

(Klein, 2003)

NOT suppressed by

Statement

Reference

l(2)gd1^d7 has phenotype, non-suppressible by Rab5^Tub.CFP

(Schneider et al., 2013)

l(2)gd1^d7 has phenotype, non-suppressible by Rab7^Tub.Venus

(Schneider et al., 2013)

l(2)gd1^d7 has phenotype, non-suppressible by kuz^ES24

(Schneider et al., 2013)

l(2)gd1^d7 has phenotype, non-suppressible by Hsap\LAMP1^UAS.EGFP/Scer\GAL4^hh-Gal4

(Schneider et al., 2013)

Enhancer of

Statement

Reference

l(2)gd1[+]/l(2)gd1^d7 is an enhancer of female germline stem cell phenotype of shrb^G5

(Matias et al., 2015)

l(2)gd1^d7 is an enhancer of wing disc phenotype of N^Ax-M1

(Klein, 2003)

NOT Enhancer of

Statement

Reference

l(2)gd1^d7 is a non-enhancer of eye disc | increased number phenotype of Psn^C1

(Klein, 2003)

l(2)gd1^d7 is a non-enhancer of sensory mother cell | increased number phenotype of Psn^C1

(Klein, 2003)

Suppressor of

Statement

Reference

l(2)gd1[+]/l(2)gd1^d7 is a suppressor of egg chamber phenotype of shrb^G5

(Matias et al., 2015)

l(2)gd1[+]/l(2)gd1^d7 is a suppressor of female germline cell phenotype of shrb^G5

(Matias et al., 2015)

l(2)gd1^d7 is a suppressor of arista | increased number phenotype of obk¹

(Atallah et al., 2004)

l(2)gd1^d7 is a suppressor of wing disc phenotype of Ser^CS94/Ser^RX106

(Klein, 2003)

NOT Suppressor of

Statement

Reference

l(2)gd1^d7 is a non-suppressor of eye disc | increased number phenotype of Psn^C1

(Klein, 2003)

l(2)gd1^d7 is a non-suppressor of sensory mother cell | increased number phenotype of Psn^C1

(Klein, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The fraction of egg chambers containing 32 cells is reduced in l(2)gd1^d7/shrb^G5 double heterozygotes compared to shrb^G5 heterozygotes. The fraction of germaria containing 'stem cysts' in which the female germline stem cells are interconnected to daughter cells is increased in l(2)gd1^d7/shrb^G5 double heterozygotes compared to shrb^G5 heterozygotes.

(Matias et al., 2015)

Rab5^{αTub84B.T:Avic\GFP-ECFP} or Rab7^{αTub84B.T:Avic\GFP-EYFP} do not rescue the ectopic N activation phenotype (ectopic expression of wg) in l(2)gd1^d7 wing discs.

Scer\GAL4^hh-Gal4-mediated expression of tkv^{Scer\UAS.T:Avic\GFP}, N^{intra.Scer\UAS.T:Avic\GFP-EGFP,T:Mmus\Cd8a} or T48^{Scer\UAS.T:Avic\GFP} suppresses the ectopic N activation phenotype (ectopic expression of wg) in the posterior of l(2)gd1^d7 wing discs. In contrast, expression of N^Scer\UAS.cUa enhances the phenotype, while Hsap\LAMP1^{Scer\UAS.T:Avic\GFP-EGFP} has no effect.

The ectopic N activation phenotype (ectopic expression of Ecol\lacZ^{Ddc.be2.E(spl)m8-HLH}) in l(2)gd1^d7 wing disk clones is suppressed if cells are also mutant for Hrs^D28 or aph-1^D35, but not for kuz^ES24.

Heterozygosity for aph-1^D35suppresses the ectopic N activation phenotype (ectopic expression of wg) in l(2)gd1^d7 wing discs.

Scer\GAL4^hh-Gal4-mediated expression of Vha68-1^GD7717 suppresses the ectopic N activation phenotype (ectopic expression of wg) in the posterior of l(2)gd1^d7 wing discs.

Scer\GAL4^hh-Gal4-mediated expression of N^{ΔEGF1-36.Scer\UAS.T:Hsim\VP16,T:Ecol\lexA} enhances while N^{BC.Scer\UAS.T:Ecol\lexA} suppresses the ectopic N activation phenotype (ectopic expression of wg) in the posterior of l(2)gd1^d7 wing discs.

Scer\GAL4^hh-Gal4-mediated expression of Rab7^GD11800 suppresses the ectopic N activation phenotype (ectopic expression of wg) in the posterior of l(2)gd1^d7 wing discs.

Homozygosity of car¹ suppresses the ectopic N activation phenotype (ectopic expression of wg) in l(2)gd1^d7 wing discs.

Scer\GAL4^hh-Gal4-mediated expression of Vps39^GD12152 suppresses the ectopic N activation phenotype (ectopic expression of wg) in the posterior of l(2)gd1^d7 wing discs.

(Schneider et al., 2013)

The ectopic upregulation of N signaling seen in l(2)gd1^d7 clones is suppressed when clones also carry the Hrs^D28 mutation.

(Childress et al., 2006)

Heterozygosity for Psn^C1 weakens the l(2)gd1^SH0495/l(2)gd1^d7 leg phenotypes.

Wing pouch clones double mutant for l(2)gd1^d7 and Su(H)⁸ show the same increase in cell death as observed in l(2)gd1^d7 clones.

(Jaekel and Klein, 2006)

The proliferation defects seen in l(2)gd1^d7 wing disc cells are suppressed by the addition of Psn^C1. N^Ax-M1, l(2)gd1^d7 mutant wing discs show an extreme phenotype where the disc loses all visible organisation and forms a rounded ball of cells. The neurogenic phenotype seen in Psn^C1 is unaffected by the addition of l(2)gd1^d7. The small eye disc phenotype also resembles that of Psn^C1.

(Klein, 2003)

Xenogenetic Interactions

Statement

Reference

Scer\GAL4^hh-Gal4-mediated expression of Hsap\LAMP1^{Scer\UAS.T:Avic\GFP-EGFP} has no effect on the ectopic N activation phenotype (ectopic expression of wg) in the posterior of l(2)gd1^d7 wing discs.

(Schneider et al., 2013)

Complementation and Rescue Data

Rescued by

l(2)gd1^d7/l(2)gd1^SH0495 is rescued by l(2)gd1^{ΔC2.Tag:NES(Unk),Tag:HA}

(Troost et al., 2012)

l(2)gd1^d7 is rescued by l(2)gd1^GFP

(Troost et al., 2012)

l(2)gd1^d7 is rescued by l(2)gd1^{ΔDM14-1.Tag:HA}

(Troost et al., 2012)

l(2)gd1^d7 is rescued by l(2)gd1^{ΔDM14-4.Tag:HA}

(Troost et al., 2012)

l(2)gd1^d7 is rescued by l(2)gd1^{ΔDM14-1+2.Tag:HA}

(Troost et al., 2012)

l(2)gd1^d7 is rescued by l(2)gd1^{ΔDM14-3+4.Tag:HA}

(Troost et al., 2012)

l(2)gd1^d7 is rescued by l(2)gd1^{ΔDM14-1+4.Tag:HA}

(Troost et al., 2012)

l(2)gd1^d7 is rescued by l(2)gd1^{ΔDM14-2+3.Tag:HA}

(Troost et al., 2012)

l(2)gd1^d7 is rescued by Scer\GAL4^da.G32/l(2)gd1^UAS.cJa

(Jaekel and Klein, 2006)

l(2)gd1^d7 is rescued by l(2)gd1^{UAS.cJa.Tag:HA}/Scer\GAL4^da.G32

(Jaekel and Klein, 2006)

Partially rescued by

l(2)gd1^d7 is partially rescued by l(2)gd1^{ΔDM14-1+3.Tag:HA}

(Troost et al., 2012)

l(2)gd1^d7 is partially rescued by l(2)gd1^ΔC2.Tag:HA

(Troost et al., 2012)

l(2)gd1^d7 is partially rescued by l(2)gd1^{ΔC2.Tag:NES(Unk),Tag:HA}

(Troost et al., 2012)

Not rescued by

l(2)gd1^d7 is not rescued by l(2)gd1^{ΔDM14.Tag:HA}

(Troost et al., 2012)

l(2)gd1^d7 is not rescued by l(2)gd1^{ΔDM14ΔC2.Tag:NES(Unk),Tag:HA}

(Troost et al., 2012)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25087

w^*; P{neoFRT}40A l(2)gd1^d7/CyO

Notes on Origin

Discoverer

Comments

l(2)gd1¹, l(2)gd1⁴, l(2)gd1^d7 and l(2)gd1^d10 all harbor the same lesion.

(Jaekel and Klein, 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

l(2)gd1^d7

l(2)gd^d7

(Atallah et al., 2004)

lgd^d7

(Hermant et al., 2024, Baeumers et al., 2020, Drusenheimer et al., 2015, Matias et al., 2015, Couturier et al., 2014, Schneider et al., 2013, Troost et al., 2012, Tyler et al., 2007, Childress et al., 2006, Gallagher and Knoblich, 2006, Jaekel and Klein, 2006)

Name Synonyms

Secondary FlyBase IDs

References (15)

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