FlyBase Allele Report: Dmel\fj[N7]

General Information

Symbol

Dmel\fj^N7

Species

D. melanogaster

Name

FlyBase ID

FBal0127230

Feature type

allele

Associated gene

Dmel\fj

Associated Insertion(s)

P{?lacW}fj^N7

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Simon, 2004)

Mutagen

P-element activity

(Katz, 2001.12.11)

Progenitor genotype

fj^9-II

(Katz, 2001.12.11)

Associated Insertion(s)

P{?lacW}fj^N7

Cytology

Description

Imprecise excision of the P{lacW} element, resulting in a complex rearrangement that retains the Ecol\lacZ marker gene.

(Katz, 2001.12.11)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | somatic clone

(Yang et al., 2002)

visible

(Buckles et al., 2001)

Phenotype Manifest In

leg joint

(Buckles et al., 2001)

ommatidium | somatic clone

(Yang et al., 2002)

photoreceptor cell R3 | somatic clone

(Yang et al., 2002)

photoreceptor cell R4 | ectopic | somatic clone

(Yang et al., 2002)

tarsal segment 2

(Buckles et al., 2001)

tarsal segment 3

(Buckles et al., 2001)

Detailed Description

Statement

Reference

An equator can easily be discerned in fj^d1/fj^N7 eyes. 98.5% of ommatidia are correctly constructed and rotated in these animals. Flies expressing fj^Scer\UAS.cZa under the control of Scer\GAL4^αTub84B.PL in a fj^d1/fj^N7 background have a normal overall pattern of polarisation in the eye; an equator can easily be discerned. 92.9% of ommatidia are correctly constructed and rotated in these animals. Expression of fj^Scer\UAS.cZa under the control of Scer\GAL4^bi-omb3-GAL4 in a fj^d1/fj^N7 background has a profound effect on ommatidial polarity; near the dorsal pole, large-scale reversals of ommatidial polarity are seen.

(Simon, 2004)

When homozygous mutant clones are made specifically in the equatorial R3/R4 precursor, most (71%) of the consequent ommatidia are in the reverse polarity. When clones are made specifically in the polar R3/R4 precursor, almost all (94%) of the consequent ommatidia are in the wild-type polarity. The mutant photoreceptor precursor eventually becomes an R4 photoreceptor 84% of the time (50% in wild-type).

(Yang et al., 2002)

The joint between tarsal segments 2 and 3 is incomplete in 96.7% of cases and fused in 3.3% of cases in homozygous flies.

(Buckles et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

fj^N7 has visible phenotype, enhanceable by +/Df(3L)st-j7

(Buckles et al., 2001)

fj^N7 has visible phenotype, enhanceable by dachs²¹⁰/d[+]

(Buckles et al., 2001)

fj^N7 has visible phenotype, enhanceable by Ser^CS94/Ser[+]

(Buckles et al., 2001)

fj^N7 has visible phenotype, enhanceable by Ser[+]/Ser^RX106

(Buckles et al., 2001)

fj^N7 has visible phenotype, enhanceable by ena[+]/ena²¹⁰

(Buckles et al., 2001)

fj^N7 has visible phenotype, enhanceable by ena²³/ena[+]

(Buckles et al., 2001)

Other

Statement

Reference

ds^38k/ds^UAO71, fj^N7/fj^d1 has abnormal planar polarity phenotype

(Simon, 2004)

ds^UAO71, fj^N7 has abnormal cell polarity | somatic clone phenotype

(Yang et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

fj^N7 has leg joint phenotype, enhanceable by +/Df(3L)st-j7

(Buckles et al., 2001)

fj^N7 has leg joint phenotype, enhanceable by dachs²¹⁰/d[+]

(Buckles et al., 2001)

fj^N7 has leg joint phenotype, enhanceable by Ser^CS94/Ser[+]

(Buckles et al., 2001)

fj^N7 has leg joint phenotype, enhanceable by Ser[+]/Ser^RX106

(Buckles et al., 2001)

fj^N7 has leg joint phenotype, enhanceable by ena[+]/ena²¹⁰

(Buckles et al., 2001)

fj^N7 has leg joint phenotype, enhanceable by ena²³/ena[+]

(Buckles et al., 2001)

Suppressor of

Statement

Reference

fj^N7 is a suppressor of photoreceptor cell R3 | somatic clone phenotype of ds^UAO71

(Yang et al., 2002)

fj^N7 is a suppressor of photoreceptor cell R4 | ectopic | somatic clone phenotype of ds^UAO71

(Yang et al., 2002)

Other

Statement

Reference

ds^38k/ds^UAO71, fj^N7/fj^d1 has wing blade phenotype

(Simon, 2004)

ds^UAO71, fj^N7 has ommatidium | somatic clone phenotype

(Yang et al., 2002)

ds^UAO71, fj^N7 has photoreceptor cell R3 | somatic clone phenotype

(Yang et al., 2002)

ds^UAO71, fj^N7 has photoreceptor cell R4 | ectopic | somatic clone phenotype

(Yang et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

ds^38k fj^N7/ds^UAO71 fj^d1 flies have disorganised ommatidial polarity; no obvious equator is present. Flies expressing both fj^Scer\UAS.cZa and ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^αTub84B.PL in a ds^38k fj^N7/ds^UAO71 fj^d1 background have a disorganised ommatidial polarity; no obvious equator is present. Flies expressing ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^αTub84B.PL in a ds^38k fj^N7/ds^UAO71 fj^d1 background have a disorganised ommatidial polarity; no obvious equator is present. Flies expressing fj^Scer\UAS.cZa under the control of Scer\GAL4^αTub84B.PL in a ds^38k fj^N7/ds^UAO71 fj^d1 background have a disorganised ommatidial polarity; no obvious equator is present. Flies expressing ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^fj-VG1 in a ds^UAO71 fj^VG1/ds^38k fj^N7 background have a disorganised ommatidial polarity; the eye contains a clearly perceptible equator in which most of the nearby ommatidia are reversed in polarity and thus point away from the equator. The polarity reversals in the eye seen in flies expressing ft^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^bi-omb3-GAL4 is strengthened in a fj^N7/fj^d1 background. Flies expressing both fj^Scer\UAS.cZa and ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^αTub84B.PL in a ds^38k fj^N7/ds^UAO71 fj^d1 background have normal polarity throughout the wing blade with the exception of a small region along the anterior proximal margin of the wing, where a small swirl is often present. Flies expressing ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^αTub84B.PL in a ds^38k fj^N7/ds^UAO71 fj^d1 background have normal polarity throughout the wing blade with the exception of a small region along the anterior proximal margin of the wing, where a small swirl is often present.

(Simon, 2004)

When ds^UAO71 homozygous mutant clones are made specifically in the equatorial R3/R4 precursors of fj^N7 mutant flies, almost all (92%) of the consequent ommatidia are in the wild-type polarity. When clones are made specifically in the polar R3/R4 precursor, most (73%) of the consequent ommatidia are in the reverse polarity. The mutant photoreceptor precursor eventually becomes an R3 photoreceptor 85% of the time (50% in wild-type).

(Yang et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6391

w^*; fj^N7/CyO, P{sevRas1.V12}FK1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

fj^N7

(Casal et al., 2006)

fjⁿ⁷

(Buckles et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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