Date: Tue, 11 Dec 2001 11:53:59 \-0500 To: flybase-updates@XXXX From: Kevin Cook <kcook@XXXX> Subject: fj and d alleles The following information accompanied stocks donated to the Bloomington Stock Center by Flora Katz, Texas A&M University (9/01). fjN7 (FBal0127230) was derived from P{lacW}fj9-II (FBal0045640) by imprecise excision. It is a complex rearrangement that retains the lacZ marker. lacZ expression is stronger than in the parental enhancer trap. It gives a loss of cells between the crossveins of the wing similar to strong fj alleles. dGC2 is a weak hypomorph with respect to growth and segmentation, but gives a strong malformed leg phenotype. It was generated in Michael Hoffmann's lab by gamma mutagenesis. It has a deletion in a unique N-terminal pre-head domain that removes a leucine zipper. dGC13 was generated in the same irradiation screen and has an eleven base pair deletion in the myosin head domain that causes a frameshift and stop codon. It acts like a null allele. d210 (FBal0127240) was also generated in Michael Hoffmanns lab by EMS treatment. It has a point mutation causing a stop codon in the myosin head domain. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-855-5782 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-3700 kcook@XXXX
