FlyBase Allele Report: Dmel\trc[1]

General Information

Symbol

Dmel\trc¹

Species

D. melanogaster

Name

FlyBase ID

FBal0017031

Feature type

allele

Associated gene

Dmel\trc

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Emoto et al., 2006)

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Amino acid replacement: R395P.

(Geng et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:19,832,341..19,832,341 [-]

Nucleotide change:

G19832341C

Amino acid change:

R391P | trc-PA; R395P | trc-PB

Reported amino acid change:

R395P

Comment:

Position of mutation on reference sequence inferred by FlyBase curator. Difference in amino acid position due to slightly different predicted cds used by authors.

(Geng et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | pupal stage (with trc⁸)

(Fei et al., 2002)

abnormal neuroanatomy | third instar larval stage (with Df(3L)BSC445)

(Han et al., 2012)

abnormal planar polarity | somatic clone

(Lee and Adler, 2002)

visible | somatic clone (with trc⁸)

(Geng et al., 2000)

Phenotype Manifest In

antenna (with trc⁸)

(Geng et al., 2000)

arista lateral (with trc⁸)

(Fei et al., 2002)

chaeta

dendrite | cell autonomous | somatic clone | increased number

(Emoto et al., 2004)

denticle (with Df(3L)kto2)

(Geng et al., 2000)

egg | somatic clone

(Horne-Badovinac et al., 2012)

larval dorsal multidendritic neuron ddaC | third instar larval stage (with Df(3L)BSC445)

(Han et al., 2012)

multidendritic neuron & dendrite

(Emoto et al., 2004)

multidendritic neuron & dendrite | somatic clone

(Emoto et al., 2004)

multidendritic neuron & dendrite | supernumerary

(Emoto et al., 2004)

ventral denticle belt (with Df(3L)kto2)

(Geng et al., 2000)

wing hair | somatic clone

(Lee and Adler, 2002)

wing hair | somatic clone | increased number (with trc⁸)

(Geng et al., 2000)

Detailed Description

Statement

Reference

trc¹/Df(3L)BSC445 mutant third instar larvae show a significant increase in the proportion of dorsal midline ddaC dendrite length that is enclosed within the epidermis rather than attached to the ECM. The amount of enclosed dendrite seen in each heterozygote is similar to wild type. Most of the dendritic crossings in these mutants are between enclosed dendrites and dendrites attached to the ECM and are thus non-contacting.

(Han et al., 2012)

Females carrying homozygous follicle cell clones can produce round eggs.

(Horne-Badovinac et al., 2012)

trc¹/+ heterozygotes exhibit wild-type dendritic tiling, with wild-type levels of dendritic crossing points per υm[2].

(Emoto et al., 2006)

trc¹ mutants exhibit supernumerary terminal branching and defective dendritic tiling. Mutant larvae show an increase in terminal branch number, and the branches of ddaC neurons often overlap each other. This tiling defect can be seen independently from the overbranching defect. The crossing branches have rigid and straight trajectories. When somatic clones are made in the neurons, mutant ddaE, IdaA and ddaC dendrites display a 50% increase in the number of branches. Mutant clones also exhibit a tiling defect. In mutants, the v'ada and vdaB dendrites often invade neighbouring fields. Major branches as well as terminal branches overlap extensively.

(Emoto et al., 2004)

trc¹/trc⁸ mutant show a variable delay in developmental rate.

trc¹/trc⁸ mutant pupae can have multiply split laterals on the aristae. Laterals are seen to split at a wide range of developmental stages. As development proceeds, the distance from the proximal base of the lateral to the proximal most branch-point increases, as does the distance between the proximal and distal branch-points. An increase in the length of the arms distal to branch-points is also seen.

(Fei et al., 2002)

trc¹/trc⁸ clones in the wing produce a weak multiple hair cell phenotype. Some hairs are split distally. The hairs appear clustered close together. In some cases the hairs are oriented almost orthogonally to the plane of the wing. trc¹/Df(3L)kto2 larvae have an abnormal pattern of denticles, with the wild-type fairly precise rows of denticles being replaced by a more chaotic arrangement. At the level of the individual denticle, the predominant abnormality is splitting (more than 30% of denticles in some denticle bands show this phenotype). trc¹/trc⁸ flies routinely show branching of one or more of the lateral extensions of the antenna.

(Geng et al., 2000)

Mutant wing and notum have rosettes of three or more short trichomes instead of single long hairs as in wild type. Useful as cell marker. Mutant cell autonomous in mitotic cells in a wild-type background, but not in a Minute background (Vinson and Adler, 1987, D. I. S. 66).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Df(2R)Sema2b-C4, trc¹ has abnormal neuroanatomy | third instar larval stage phenotype

(Meltzer et al., 2016)

raw^jj102, trc¹/trc[+] has abnormal neuroanatomy | somatic clone phenotype

(Lee et al., 2015)

Mob2^H4-5, trc¹/trc[+] has abnormal neuroanatomy | third instar larval stage phenotype

(Campbell and Ganetzky, 2013)

stan[+]/stan^E59, trc¹ has abnormal neuroanatomy | third instar larval stage phenotype

(Matsubara et al., 2011)

mTor^ΔP, trc¹/trc[+] has abnormal neuroanatomy phenotype

(Koike-Kumagai et al., 2009)

Sin1^e03756, trc¹/trc[+] has abnormal neuroanatomy phenotype

(Koike-Kumagai et al., 2009)

rictor^Δ2, trc¹/trc[+] has abnormal neuroanatomy phenotype

(Koike-Kumagai et al., 2009)

hpo^MGH4, trc¹/trc[+] has abnormal neuroanatomy phenotype

(Emoto et al., 2006)

hpo^KC202, trc¹/trc[+] has abnormal neuroanatomy phenotype

(Emoto et al., 2006)

hpo^MGH4/hpo[+], trc¹ has abnormal neuroanatomy phenotype

(Emoto et al., 2006)

hpo^KC202/hpo[+], trc¹ has abnormal neuroanatomy phenotype

(Emoto et al., 2006)

fry²/fry[+], mats^e235, trc¹/trc[+] has visible | dominant phenotype

(He et al., 2005)

fry²/fry[+], mats^e03077, trc¹/trc[+] has visible | dominant phenotype

(He et al., 2005)

Phenotype Manifest In

Enhancer of

Statement

Reference

trc¹ is an enhancer of wing hair phenotype of ultA¹

(Lee and Adler, 2002)

Other

Statement

Reference

Df(2R)Sema2b-C4, trc¹ has larval multidendritic class IV neuron | third instar larval stage phenotype

(Meltzer et al., 2016)

Df(2R)Sema2b-C4, trc¹ has dendrite | third instar larval stage phenotype

(Meltzer et al., 2016)

raw^jj102, trc¹/trc[+] has dendrite | somatic clone phenotype

(Lee et al., 2015)

raw^jj102, trc¹/trc[+] has larval multidendritic class IV neuron | somatic clone phenotype

(Lee et al., 2015)

Mob2^H4-5, trc¹/trc[+] has NMJ bouton | third instar larval stage phenotype

(Campbell and Ganetzky, 2013)

Mob2^H4-5, trc¹/trc[+] has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Campbell and Ganetzky, 2013)

stan[+]/stan^E59, trc¹ has dendrite | third instar larval stage phenotype

(Matsubara et al., 2011)

stan[+]/stan^E59, trc¹ has larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype

(Matsubara et al., 2011)

mTor^ΔP, trc¹/trc[+] has dendrite phenotype

(Koike-Kumagai et al., 2009)

Sin1^e03756, trc¹/trc[+] has dendrite phenotype

(Koike-Kumagai et al., 2009)

Sin1^e03756, trc¹/trc[+] has larval multidendritic class IV neuron phenotype

(Koike-Kumagai et al., 2009)

mTor^ΔP, trc¹/trc[+] has larval multidendritic class IV neuron phenotype

(Koike-Kumagai et al., 2009)

rictor^Δ2, trc¹/trc[+] has dendrite phenotype

(Koike-Kumagai et al., 2009)

rictor^Δ2, trc¹/trc[+] has larval multidendritic class IV neuron phenotype

(Koike-Kumagai et al., 2009)

hpo^MGH4, trc¹/trc[+] has multidendritic dendrite phenotype

(Emoto et al., 2006)

hpo^KC202, trc¹/trc[+] has multidendritic dendrite phenotype

(Emoto et al., 2006)

hpo^MGH4/hpo[+], trc¹ has multidendritic dendrite phenotype

(Emoto et al., 2006)

hpo^KC202/hpo[+], trc¹ has multidendritic dendrite phenotype

(Emoto et al., 2006)

fry²/fry[+], mats^e235, trc¹/trc[+] has wing hair | increased number phenotype

(He et al., 2005)

fry²/fry[+], mats^e03077, trc¹/trc[+] has wing hair | increased number phenotype

(He et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The level of non-contacting dendrite crossing in class IV dendrite arborizing larval neurons is significantly increased in trc¹;Df(2R)Sema-2b-C4 double mutants compared to either trc¹/+ or Df(2R)Sema-2b-C4/+ heterozygotes.

(Meltzer et al., 2016)

trc¹/Mob2^H4-5 double heterozygous third instar larvae show a neuromuscular junction (NMJ) overgrowth phenotype comparable to that seen in Mob2^H4-5 single homozygotes: both bouton number and branch points per NMJ are increased compared to wild type.

(Campbell and Ganetzky, 2013)

stan^E59/trc¹ double heterozygous larvae show increased crossing of dendrites in the dendritic arbor of the ddaC class IV neurons compared to wild type (neither single heterozygote shows this phenotype).

(Matsubara et al., 2011)

Class IV da neurons of Sin1^e03756/+ ; trc¹/+, rictor^Δ2/+ ; trc¹/+ and Tor^ΔP/+ ; trc¹/+ double heterozygous larvae show defects in dendritic tiling, with a significantly higher number of dendritic branches crossing one another compared to wild type.

(Koike-Kumagai et al., 2009)

trc¹/+ tutl²³/+ double heterozygotes do not show any enhanced class I da neuron branching defects or class IV neuron dendrite self-avoidance defects compared to tutl²³/+ single heterozygotes.

(Long et al., 2009)

Transheterozygotes for trc¹/hpo^MGH4 exhibit obvious iso-neuronal as well as hetero-neuronal tiling defects, including a significant increase in dendritic crossing-points compared to single mutants.

Transheterozygotes for wts^x1/trc¹ do not show any significant dendritic phenotypes.

Transheterozygotes for trc¹/hpo^KC202 exhibit obvious iso-neuronal as well as hetero-neuronal tiling defects, including a significant increase in dendritic crossing-points compared to single mutants.

(Emoto et al., 2006)

fry² trc¹ mats^e235 triple heterozygotes have a weak multiple wing hair phenotype in 48% of wings.

(He et al., 2005)

fry¹/trc¹ larvae exhibit tiling defects, though they have normal dendritic length and branch points.

(Emoto et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

trc¹ is rescued by trc^UAS.Tag:FLAG/Scer\GAL4⁴⁷⁷

(Emoto et al., 2004)

Not rescued by

trc¹ is not rescued by trc^{K122A.UAS.L.Tag:FLAG}/Scer\GAL4⁴⁷⁷

(Emoto et al., 2004)

trc¹ is not rescued by trc^{S292A.T449A.UAS.Tag:FLAG}/Scer\GAL4⁴⁷⁷

(Emoto et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

1135

ru¹ jv¹ trc¹ ca¹/TM3, Ser¹

5261

y¹; trc¹ P{neoFRT}80B kar² ry⁵⁰⁶/TM6C, ry^CB Sb¹ Tb¹

Kyoto

106218

ru¹ jv¹ trc¹ ca¹/TM3, Ser¹

108130

y¹; trc¹ P{neoFRT}80B kar² ry⁵⁰⁶/TM6C, ry^CB Sb¹ Tb¹

Notes on Origin

Discoverer

A. Ferrus.

(Martin, 1995.12.5)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

trc¹

(Rui et al., 2020, Poon et al., 2018, Meltzer et al., 2016, Lee et al., 2015, Campbell and Ganetzky, 2013, Han et al., 2012, Horne-Badovinac et al., 2012, Matsubara et al., 2011, Koike-Kumagai et al., 2009, Long et al., 2009, Emoto et al., 2006, He et al., 2005, Fei et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

Open Close