FlyBase Allele Report: Dmel\raw[jj102]

General Information

Symbol

Dmel\raw^jj102

Species

D. melanogaster

Name

FlyBase ID

FBal0302480

Feature type

allele

Associated gene

Dmel\raw

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Lee et al., 2015)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q777term.

(Lee et al., 2015)

Nucleotide substitution: C?T.

(Lee et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:8,710,524..8,710,524 [-]

Nucleotide change:

C8710524T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

Q777term

Comment:

Mapped relative to isoform raw-PB, the only isoform with a Q at amino acid position 777.

(Lee et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone

(Lee et al., 2015)

lethal - all die during embryonic stage

(Lee et al., 2015)

Phenotype Manifest In

dendrite | somatic clone

(Lee et al., 2015)

larval multidendritic class IV neuron | somatic clone

(Lee et al., 2015)

Detailed Description

Statement

Reference

raw^jj102 mutant clones in class IV dendritic arborizing neuron (Cd4a) neurons exhibit defects in dendrite stability, with frequency of retraction matching growth. Terminal dendrites are short, mostly unbranched and exhibit reduced complexity in distal regions of the arbor. The average terminal dendrite length is significantly reduced. Terminal dendrite orientation is altered and they often grow in proximity to primary dendrites, rather than growing away from them. Dendrite-dendrite crossing events are common.

Homozygous raw^jj102 mutants arrest as embryos with dorsal closure defects.

raw^jj102 mutant terminal dendrites are enriched in F-actin compared to controls. The microtubule cytoskeleton is unaffected.

(Lee et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

raw^jj102 has abnormal neuroanatomy | somatic clone phenotype, enhanceable by trc^UAS.cUa/Scer\GAL4^109(2)80

(Lee et al., 2015)

Suppressed by

Statement

Reference

raw^jj102 has abnormal neuroanatomy | somatic clone phenotype, suppressible by Scer\GAL4^109(2)80/trc^{UAS.Tag:Myr(Src64B)}

(Lee et al., 2015)

raw^jj102 has abnormal neuroanatomy | somatic clone phenotype, suppressible by Scer\GAL4^109(2)80/trc^T453E.UAS.L

(Lee et al., 2015)

raw^jj102 has abnormal neuroanatomy | somatic clone phenotype, suppressible by mew^UAS.cMBa/Scer\GAL4^elav-C155/mys^UAS.cDa

(Lee et al., 2015)

Other

Statement

Reference

raw^jj102, trc¹/trc[+] has abnormal neuroanatomy | somatic clone phenotype

(Lee et al., 2015)

AGO1^jj472, raw^jj102/raw[+] has abnormal neuroanatomy phenotype

(Lee et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

raw^jj102 has larval multidendritic class IV neuron | somatic clone phenotype, enhanceable by trc^UAS.cUa/Scer\GAL4^109(2)80

(Lee et al., 2015)

raw^jj102 has dendrite | somatic clone phenotype, enhanceable by trc^UAS.cUa/Scer\GAL4^109(2)80

(Lee et al., 2015)

Suppressed by

Statement

Reference

raw^jj102 has larval multidendritic class IV neuron | somatic clone phenotype, suppressible by Scer\GAL4^109(2)80/trc^{UAS.Tag:Myr(Src64B)}

(Lee et al., 2015)

raw^jj102 has dendrite | somatic clone phenotype, suppressible by Scer\GAL4^109(2)80/trc^{UAS.Tag:Myr(Src64B)}

(Lee et al., 2015)

raw^jj102 has larval multidendritic class IV neuron | somatic clone phenotype, suppressible by Scer\GAL4^109(2)80/trc^T453E.UAS.L

(Lee et al., 2015)

raw^jj102 has dendrite | somatic clone phenotype, suppressible by Scer\GAL4^109(2)80/trc^T453E.UAS.L

(Lee et al., 2015)

raw^jj102 has larval multidendritic class IV neuron | somatic clone phenotype, suppressible by mew^UAS.cMBa/Scer\GAL4^elav-C155/mys^UAS.cDa

(Lee et al., 2015)

raw^jj102 has dendrite | somatic clone phenotype, suppressible by mew^UAS.cMBa/Scer\GAL4^elav-C155/mys^UAS.cDa

(Lee et al., 2015)

Other

Statement

Reference

raw^jj102, trc¹/trc[+] has dendrite | somatic clone phenotype

(Lee et al., 2015)

raw^jj102, trc¹/trc[+] has larval multidendritic class IV neuron | somatic clone phenotype

(Lee et al., 2015)

AGO1^jj472, raw^jj102/raw[+] has dendrite phenotype

(Lee et al., 2015)

AGO1^jj472, raw^jj102/raw[+] has larval multidendritic class IV neuron phenotype

(Lee et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Larvae doubly heterozygous for raw^jj102 and trc¹ exhibit significant increased in dendrite-dendrite crossing in class IV dendritic arborizing neurons. Neither heterozygote alone has a significant effect on the number of crossing events. Dendrite number is unaffected.

Expression of trc^Scer\UAS.cUa under the control of Scer\GAL4^109(2)80 enhances the number of dendrite-dendrite crossings seen in raw^jj102 mutant class IV dendritic arborizing neuron clones.

Expression of trc^{Scer\UAS.T:Myr-Src64B} under the control of Scer\GAL4^109(2)80 suppresses the number of dendrite-dendrite crossings seen in raw^jj102 mutant class IV dendritic arborizing neuron clones.

Expression of trc^{T453E.Scer\UAS} under the control of Scer\GAL4^109(2)80 partially suppresses the number of dendrite-dendrite crossings seen in raw^jj102 mutant class IV dendritic arborizing neuron clones.

Co-expression of mys^Scer\UAS.cDa and mew^{Scer\UAS.cMBa} under the control of Scer\GAL4^elav-C155 suppresses the number of dendrite-dendrite crossings seen in raw^jj102 mutant class IV dendritic arborizing neuron clones. Terminal branch number is significantly restored and a greater fraction of stable terminals are seen compared. However, the mutant terminal dendrite elongation defect is not rescued.

Larvae doubly heterozygous for raw^jj102/AGO1^jj472 class IV arborizing dendritic neuron clones have shorter terminal dendrites and less complex terminal arbors than controls. The frequency of dendrite-dendrite crossing over is similar to controls.

(Lee et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

raw^jj102 is rescued by Scer\GAL4^109(2)80/raw^{UAS.short.EGFP}

(Lee et al., 2015)

Comments

Expression of raw^{Scer\UAS.short.T:Avic\GFP-EGFP} under the control of Scer\GAL4^109(2)80 fully rescues the dendrite defects seen in raw^jj102 mutant class IV dendritic arborizing neuron clones.

(Lee et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

raw^jj102

(Lee et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close