FlyBase Allele Report: Dmel\mew[UAS.cMBa]

General Information

Symbol

Dmel\mew^UAS.cMBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Martin-Bermudo

FlyBase ID

FBal0062567

Feature type

allele

Associated gene

Dmel\mew

Associated Insertion(s)

Carried in Construct

P{UAS-mew.MB}

Also Known As

UAS-mew, UAS-αPS1

Key Links

Transgenic product class

wild_type

(Martin-Bermudo et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Martin-Bermudo et al., 1997)

Mutagen

in vitro construct

(Martin-Bermudo et al., 1997)

Progenitor genotype

Carried in construct

P{UAS-mew.MB}

Cytology

Description

mew is expressed under the control of UAS regulatory sequences.

(Martin-Bermudo et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mew

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of mew^{Scer\UAS.cMBa} in class IV da neurons under the control of Scer\GAL4^ppk.PH does not result in dendritic reduction.

(Han et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

mew^UAS.cMBa, Scer\GAL4^elav-C155, mys^UAS.cDa is a suppressor of abnormal neuroanatomy | somatic clone phenotype of raw^jj102

(Lee et al., 2015)

mew^UAS.cMBa, mys^UAS.cDa, Scer\GAL4^ppk.PH is a suppressor | partially of abnormal neuroanatomy phenotype of Ret^C168/Df(2L)BSC312

(Soba et al., 2015)

mew^UAS.cMBa/Scer\GAL4^ppk.PH is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^ppk.PH, mys^UAS.cDa

(Han et al., 2012)

mew^UAS.cMBa, mys^UAS.cDa, Scer\GAL4^ppk.PH is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of fry⁰²²⁴⁰/fry¹

(Han et al., 2012)

mew^UAS.cMBa, mys^UAS.cDa, Scer\GAL4^ppk.PH is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Sin1^e03756

(Han et al., 2012)

mew^UAS.cMBa, Scer\GAL4^twi.PG, Scer\GAL4^how-24B is a suppressor of lethal | recessive | embryonic stage phenotype of if^B4

(Martin-Bermudo et al., 1997)

NOT Suppressor of

Statement

Reference

mew^UAS.cMBa/Scer\GAL4^how-24B is a non-suppressor of lethal | recessive | embryonic stage phenotype of if^B4

(Martin-Bermudo et al., 1997)

Other

Statement

Reference

Scer\GAL4^ppk.PH, mew^UAS.cMBa, mys^UAS.cDa has abnormal neuroanatomy | third instar larval stage phenotype

(Han et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

mew^UAS.cMBa/Scer\GAL4^GMR.long is an enhancer of eye phenotype of Scer\GAL4^GMR.long, parvin^UASp.mGFP6

(Chountala et al., 2012)

Suppressor of

Statement

Reference

mew^UAS.cMBa, mys^UAS.cDa, Scer\GAL4^ppk.PU is a suppressor of larval multidendritic class IV neuron | third instar larval stage phenotype of Sema2b^f02042/Df(2R)Sema2b-C4

(Meltzer et al., 2016)

mew^UAS.cMBa, Scer\GAL4^elav-C155, mys^UAS.cDa is a suppressor of dendrite | somatic clone phenotype of raw^jj102

(Lee et al., 2015)

mew^UAS.cMBa, Scer\GAL4^elav-C155, mys^UAS.cDa is a suppressor of larval multidendritic class IV neuron | somatic clone phenotype of raw^jj102

(Lee et al., 2015)

mew^UAS.cMBa, mys^UAS.cDa, Scer\GAL4^ppk.PH is a suppressor | partially of larval multidendritic class IV neuron phenotype of Ret^C168/Df(2L)BSC312

(Soba et al., 2015)

mew^UAS.cMBa/Scer\GAL4^ppk.PH is a suppressor | partially of larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype of Scer\GAL4^ppk.PH, mys^UAS.cDa

(Han et al., 2012)

mew^UAS.cMBa, mys^UAS.cDa, Scer\GAL4^ppk.PH is a suppressor of larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype of fry⁰²²⁴⁰/fry¹

(Han et al., 2012)

mew^UAS.cMBa, mys^UAS.cDa, Scer\GAL4^ppk.PH is a suppressor of larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype of Sin1^e03756

(Han et al., 2012)

mew^UAS.cMBa, Scer\GAL4^twi.PG, Scer\GAL4^how-24B is a suppressor of embryonic/larval midgut | embryonic stage phenotype of if^B4

(Martin-Bermudo et al., 1997)

mew^UAS.cMBa, Scer\GAL4^twi.PG, Scer\GAL4^how-24B is a suppressor of gastric caecum phenotype of if^B4

(Martin-Bermudo et al., 1997)

NOT Suppressor of

Statement

Reference

mew^UAS.cMBa, Scer\GAL4^twi.PG, Scer\GAL4^how-24B is a non-suppressor of visceral mesoderm phenotype of if^B4

(Martin-Bermudo et al., 1999)

mew^UAS.cMBa/Scer\GAL4^how-24B is a non-suppressor of ventral longitudinal muscle cell phenotype of if^B4

(Martin-Bermudo et al., 1997)

Other

Statement

Reference

Scer\GAL4^ppk.PH, mew^UAS.cMBa, mys^UAS.cDa has larval ventral multidendritic neuron vdaB | third instar larval stage phenotype

(Han et al., 2012)

Scer\GAL4^Tub.PU, if^B4, mew^UAS.cMBa has follicle cell | somatic clone phenotype

(Delon and Brown, 2009)

Scer\GAL4^Tub.PU, if^B4, mew^UAS.cMBa has stress fiber | somatic clone phenotype

(Delon and Brown, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of mew^{Scer\UAS.cMBa} and mys^Scer\UAS.cDa under the control of Scer\GAL4^ppk.PU in the Sema-2b^f02042/Df(2R)Sema-2b-C4 mutant larvae background fully rescued the increased dendritic crossing phenotype in the class IV da neurons displayed by the Sema-2b^f02042/Df(2R)Sema-2b-C4 mutants.

(Meltzer et al., 2016)

Co-expression of mys^Scer\UAS.cDa and mew^{Scer\UAS.cMBa} under the control of Scer\GAL4^elav-C155 suppresses the number of dendrite-dendrite crossings seen in raw^jj102 mutant class IV dendritic arborizing neuron clones. Terminal branch number is significantly restored and a greater fraction of stable terminals are seen compared. However, the mutant terminal dendrite elongation defect is not rescued.

(Lee et al., 2015)

Co-expression of mys^Scer\UAS.cDa and mew^{Scer\UAS.cMBa} in class IV dendritic arborizing neurons under the control of Scer\GAL4^ppk.PH partially suppresses the dendrite growth seen in Ret^C168/Df(2L)BSC312 mutant larvae and completely suppresses the loss of dendrite-ECM interaction. The reduction in dendritic field coverage is not suppressed.

(Soba et al., 2015)

Expression of mew^{Scer\UAS.cMBa} largely rescues the dendritic reduction seen when mys^Scer\UAS.cDa is expressed in class IV da neurons under the control of Scer\GAL4^ppk.PH.

Co-expression of mys^Scer\UAS.cDa and mew^{Scer\UAS.cMBa} under the control of Scer\GAL4^ppk.PH completely eliminates the enclosure of ventral midline vdaB neurons in the epidermis. 8.43% of vdaB neurons are enclosed in wild type. All associated non-contacting dendritic crossing is also suppressed.

Co-expression of mys^Scer\UAS.cDa and mew^{Scer\UAS.cMBa} the control of Scer\GAL4^ppk.PH rescues the increase in epidermally enclosed dendrites seen in fry¹/fry⁰²²⁴⁰ mutants. The dendritic crossing seen in fry¹/fry⁰²²⁴⁰ mutants is also largely rescued. The dendritic crossings that remain in the rescue flies are between two ECM attached dendrites, rather than non-contacting crossings between an epidermally enclosed dendrite and one attached to the ECM. The crossing on the v'ada/vdaB interface is also mostly rescued and all non-contacting crossing is eliminated.

(Han et al., 2012)

Expression of mew^{Scer\UAS.cMBa} in if^B4 follicle cell clones (using the MARCM method) results in a delay in the developmental reorientation of stress fibres.

(Delon and Brown, 2009)

Expression of mew^{Scer\UAS.cMBa} under the control of both Scer\GAL4^twi.PG and Scer\GAL4^how-24B fails to rescue the visceral mesoderm phenotype of if^B4 embryos.

(Martin-Bermudo et al., 1999)

The embryonic lethality of if^B4 homozygotes is not rescued by mew^{Scer\UAS.cMBa} expressed using Scer\GAL4^how-24B, although it is rescued in approximately 1/3 of cases by mew^{Scer\UAS.cMBa} expressed using Scer\GAL4^how-24B in conjunction with Scer\GAL4^twi.PG. Partially rescues the midgut phenotype of if^B4 hemizygotes when expressed using Scer\GAL4^how-24B in conjunction with Scer\GAL4^twi.PG. Scer\GAL4^twi.PG if^B4/+ ; Scer\GAL4^how-24B mew^{Scer\UAS.cMBa} embryos have normal muscle attachments.