FlyBase Gene Report: Dmel\Prosα2

General Information

Symbol

Dmel\Prosα2

Species

D. melanogaster

Name

Proteasome α2 subunit

Annotation Symbol

CG5266

Feature Type

protein_coding_gene

FlyBase ID

FBgn0086134

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. (UniProt, P40301)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Pros25, α2, PROS Dm25, Su(DTS)-1, Proteasome 25kD subunit

Key Links

Genomic Location

Cytogenetic map

87B10-87B10

Sequence location

3R:12,431,321..12,432,556 [+]

Recombination map

3-52

RefSeq locus

NT_033777 REGION:12431321..12432556

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

NOT enables peptidase activity

inferred from key residues with MEROPS:T01.UNA

(GO curators, 2012-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in proteasome-mediated ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Lőw et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in proteasome-mediated ubiquitin-dependent protein catabolic process

inferred by curator from GO:0005839

(Sumegi et al., 2003)

inferred from biological aspect of ancestor with PANTHER:PTN008521424

(GO Reference Genome Project, 2011-)

involved_in ubiquitin-dependent protein catabolic process

inferred from electronic annotation with InterPro:IPR000426

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of proteasome complex

inferred from direct assay

(Nickell et al., 2009)

part_of proteasome core complex

inferred from direct assay

(Tonoki et al., 2009, Sumegi et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of proteasome core complex, alpha-subunit complex

inferred from electronic annotation with InterPro:IPR000426

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000175235

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:P40302

(Gene Ontology Curators, 2002-)

part_of proteasome core complex

inferred from electronic annotation with InterPro:IPR001353

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

PROTEASOME ALPHA SUBUNITS

26S PROTEASOME - TESTIS-SPECIFIC VARIANT

26S PROTEASOME

Protein Family (UniProt)

Belongs to the peptidase T1A family. (P40301)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

PROTEASOME ALPHA SUBUNITS -: The 26S proteasome degrades polyubiquitinated proteins in the cytoplasm and nucleus. It is formed from a cylindrical catalytic core 20S proteasome capped at each end by a regulatory 19S particle. The 19S regulatory particle recognizes, unfolds, and translocates ubiquitinated proteins into the catalytic core particle in an ATP-dependent manner. The core particle is a highly conserved, barrel-shaped complex composed of four stacked heptameric rings. Narrow substrate entry channels are created by the two outer rings, which are each formed by seven alpha subunits. (Adapted from FBrf0215459 and PMID:28583440).
26S PROTEASOME - TESTIS-SPECIFIC VARIANT -: The 26S proteasome degrades polyubiquitinated proteins in the cytoplasm and nucleus. It is formed from a cylindrical catalytic core 20S proteasome capped at each end by a regulatory 19S particle. The 19S regulatory particle recognizes, unfolds, and translocates ubiquitinated proteins into the catalytic core particle in an ATP-dependent manner. (Adapted from FBrf0215459).
26S PROTEASOME -: The 26S proteasome degrades polyubiquitinated proteins in the cytoplasm and nucleus. It is formed from a cylindrical catalytic core 20S proteasome capped at each end by a regulatory 19S particle. The 19S regulatory particle recognizes, unfolds, and translocates ubiquitinated proteins into the catalytic core particle in an ATP-dependent manner. (Adapted from FBrf0215459).

Protein Function (UniProtKB)

(UniProt, P40301)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Prosα2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P40301)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 5.55

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Prosα2-RA

FBtr0082590

NM_079604

920

234

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Prosα2-PA

FBpp0082062

25.9

234

6.68

P40301

NP_524328

AAF54814

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity). Interacts with Rpn6.

(UniProt, P40301)

Crossreferences

InterPro - A database of protein families, domains and functional sites

MEROPS - An information resource for peptidases (also termed proteases, proteinases and proteolytic enzymes) and the proteins that inhibit them.

T01.972

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P40301

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Prosα2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Prosalpha2_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.80

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

germline cell

(Fisher et al., 2012)

embryonic stage 11 -- 12

germline cell

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of proteasome complex

inferred from direct assay

(Nickell et al., 2009)

part_of proteasome core complex

inferred from direct assay

(Tonoki et al., 2009, Sumegi et al., 2003)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Prosα2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Prosα2

View exonic expression by tissue for Dmel\Prosα2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0086134

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41531/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086134

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086134

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5266

Flygut - An atlas of the Drosophila adult midgut

FBgn0086134

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Prosα2

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Prosα2

Transgenic constructs containing regulatory region of Prosα2

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene disrupted in

(Ryder, 2004.4.26)

(Ryder, 2004.4.26)

(Ryder, 2004.4.26)

(Neuburger et al., 2006)

Gene not disrupted in

Df(3R)M-Kx1

(Neuburger et al., 2006)

Df(3R)ry85

(Neuburger et al., 2006)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^GMR.PF

Prosα2^GD15087

lethal, with Scer\GAL4^ple.PF

Prosα2^GD15087

lethal, with Scer\GAL4^pnr-MD237

Prosα2^GD15087

lethal, with Scer\GAL4^tin.CΔ4

Prosα2^GD15087

lethal (with Df(3R)T-32)

Prosα2^SuDTS

lethal | larval stage | recessive

Prosα2^SuDTS

lethal | pupal stage | recessive

Prosα2^SuDTS

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

Prosα2^GD15087

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Prosα2^GD15087

Other Phenotypes

Allele

abnormal developmental rate

Prosα2^SuDTS

abnormal developmental rate (with Df(3R)T-32)

Prosα2^SuDTS

decreased occurrence of cell division | larval stage (with Df(3R)T-32)

Prosα2^SuDTS

decreased rate of movement (with Df(3R)T-32)

Prosα2^SuDTS

decreased rate of movement | larval stage | recessive

Prosα2^SuDTS

Phenotype manifest in

Allele

sarcomere, with Scer\GAL4^Mef2.PR

Prosα2^GD15087

Z disc, with Scer\GAL4^Mef2.PR

Prosα2^GD15087

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (15)

Hsap\PSMA2

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (15)

Rnor\Psma2

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (14)

Mmus\Psma2

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (9)

Xtro\psma2

7 of 13

Yes

5 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (16)

Drer\psma2a

14 of 14

Yes

Drer\psma2b

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (10)

Cele\pas-2

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (10)

Agam\AgaP_AGAP001995

12 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (19)

Atha\AT1G79210

12 of 13

Yes

Atha\PAB1

12 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (10)

Scer\PRE8

13 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (9)

Spom\pre8

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Prosα2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (24)

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PSMA2; proteasome 20S subunit alpha 2

14 of 14

176842

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Prosα2 - AnxB10

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - CG11885

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - CG12321

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - CG13319

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - CG15717

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - CG7675

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Dera

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Klc

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - PSMG1

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Pomp

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Prosα1

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Prosα3

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Prosα4

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Prosα5

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Prosα6

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Prosα7

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Prosβ1

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Prosβ2

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Prosβ3

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Prosβ4

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Prosβ4R2

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Prosβ5

anti bait coimmunoprecipitation, western blot, experimental knowledge based

(Tsakiri et al., 2019, Tsakiri et al., 2013, Tsakiri et al., 2013, Guruharsha et al., 2011)

Prosα2 - Prosβ6

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Prosβ7

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - REG

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Rpn1

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Rpn11

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Rpn13

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Rpn2

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Rpn6

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Rpn7

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Rpn8

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Rpt1

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Rpt2

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Rpt3

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Rpt4

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Rpt5

experimental knowledge based

(Guruharsha et al., 2011)

Prosα2 - Rpt6

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Prosα2

suppressible

Prosβ6

(Neuburger et al., 2006)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Debcl

enhanceable

Prosα2

(Colin et al., 2014)

Prosβ2

suppressible

Prosα2

(Neuburger et al., 2006)

Prosβ2|Prosβ6

suppressible

Prosα2

(Neuburger et al., 2006, Smyth and Belote, 1999)

Prosβ6

suppressible

Prosα2

(Neuburger et al., 2006)

External Data

Subunit Structure (UniProtKB)

(UniProt, P40301 )

Linkouts

BioGRID - A database of protein and genetic interactions.

66629

DroID - A comprehensive database of gene and protein interactions.

FBgn0086134

MIST (genetic) - An integrated Molecular Interaction Database

41531

MIST (protein-protein) - An integrated Molecular Interaction Database

41531

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Regulation of ornithine decarboxylase (ODC)

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Proteasome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-52

Cytogenetic map

87B10-87B10

Sequence location

3R:12,431,321..12,432,556 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87B10-87B10

Limits computationally determined from genome sequence between P{PZ}svp⁰⁷⁸⁴² and P{lacW}Vha55^j2E9

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

87B-87B

(determined by in situ hybridisation)

(Zaiss and Belote, 1997)

Experimentally Determined Recombination Data

Location

3-52

(FlyBase, 2016)

3-49.5

(Comeron et al., 2012)

3-48.5

(Smyth and Belote, 1999, Belote, 1998.3.17)

Left of (cM)

(Neuburger et al., 2006)

Right of (cM)

(Neuburger et al., 2006)

(Neuburger et al., 2006, Belote, 1998.3.17)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

44560

y¹ v¹; P{TRiP.HMS02856}attP40

32621

w¹¹¹⁸; P{EP}Prosα2^G8948/TM6C, Sb¹

36898

y¹ sc^* v¹ sev²¹; P{TRiP.GL01088}attP2/TM3, Sb¹

FlyORF

F001472

M{UAS-Prosα2.ORF}ZH-86Fb

F000866

M{UAS-Prosα2.ORF.3xHA}ZH-86Fb

Kyoto

122030

w¹¹¹⁸; P{EP}Prosα2^EP931

NIG-Fly

HMS02856

y¹ v¹; P{TRiP.HMS02856}attP40/CyO

VDRC

v101409

P{KK109089}VIE-260B

v29686

w¹¹¹⁸; P{GD15087}v29686

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (115)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GM13604

Other clones

DPiM_GM13604

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0086134

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0086134

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of short, monastral spindles and severe proliferation defects when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

The sequence and genomic organisation of the Pros25 locus has been determined.

(Seelig et al., 1993)

Pros25 has been cloned and sequenced.

(Seelig et al., 1993)

Relationship to Other Genes

Source for database merge of

Source for merge of: Pros25 Su(DTS)-1

(Neuburger et al., 2006)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Prosα2 Pros25

(FlyBase, 2009-)

Nomenclature comments

The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms.

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (31)

Reported As

Symbol Synonym

153303_at

(Kristensen et al., 2005)

20S-(a)

(Manola et al., 2021)

20S-α

(Nikou et al., 2019, Tsakiri et al., 2019)

Alpha-2

(Lundgren et al., 2005)

CG5266

(Kolkhof et al., 2017, Çiçek et al., 2016, Hosono et al., 2015, Fukuoh et al., 2014, Wang et al., 2014, Moutinho-Pereira et al., 2013, Schertel et al., 2013, Yano et al., 2012, Cammarato et al., 2011, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Belote and Zhong, 2009, Keleman et al., 2009.8.5, Puri et al., 2008, Barbash and Lorigan, 2007, Goshima et al., 2007, Lundgren et al., 2005, Pile et al., 2003, Ma et al., 2002)

PRC3

(Mushegian et al., 1998)

PROS Dm25

(Seelig et al., 1993, Seelig et al., 1993)

PROS-Dm25g

(Seelig, 1993.2.5)

PSA2_DROME

(Storm and Sonnhammer, 2003)

Pros α2

(Kurshakova et al., 2019)

Pros25

(Dent et al., 2015, Zang et al., 2015, Kwon et al., 2013, Lőw et al., 2013, McPhee et al., 2013, Schertel et al., 2013, Carbone et al., 2009, Puri et al., 2008, Barbash and Lorigan, 2007, Geng and MacDonald, 2007, Goshima et al., 2007, Stuart et al., 2007, Neuburger et al., 2006, Lundgren et al., 2005, Ma et al., 2002)

Prosalpha2

(Zhang and Zhang, 2019)

Prosα2

(Lee et al., 2024, Palacios et al., 2021, Walls et al., 2020, Aly et al., 2018, Zhu et al., 2013, Belote and Zhong, 2009, Ma et al., 2002)

Prot. 25 Kda

(Moutinho-Pereira et al., 2013)

Su(DTS)

(Neuburger et al., 2006)

Su(DTS)-1

alpha2

(Yano et al., 2012)

dα2

(Wojcik and DeMartino, 2002)

pros25

(Colin et al., 2014)

prosalpha2

(Blount et al., 2018)

α-2

(Wang et al., 2014)

α2

(Nguyen et al., 2019, Cho-Park and Steller, 2013, Belote and Zhong, 2009)

α2_dm

(Zaiss and Belote, 1997)

Name Synonyms

20S proteasome

(Bohm et al., 2000)

20s proteasome

(Speese et al., 2003)

Pros25

(Cammarato et al., 2011)

Proteasome 25kD subunit

(Moutinho-Pereira et al., 2013)

Proteasome subunit α-type 2

(Adrain et al., 2004)

Proteasome α2 subunit

Proteosome

(Takahashi et al., 2001)

Su(DTS)-1

α-subunit of 20S proteasome

(Fredriksson et al., 2012)

α2

(Nickell et al., 2009)

α2 subunit

(Tonoki et al., 2009)

Secondary FlyBase IDs

FBgn0010405
FBgn0024727

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 91 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41531

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P40301

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P40301

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P40301

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0086134

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41531/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086134

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086134

FlyMine - An integrated database for Drosophila genomics

FBgn0086134

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5266

MARRVEL_MODEL - MARRVEL (model organism gene)

41531

MEROPS - An information resource for peptidases (also termed proteases, proteinases and proteolytic enzymes) and the proteins that inhibit them.

T01.972

Linkouts

BioGRID - A database of protein and genetic interactions.

66629

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0086134

DroID - A comprehensive database of gene and protein interactions.

FBgn0086134

DRSC - Results frm RNAi screens

FBgn0086134

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Prosalpha2_1

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0086134

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5266

Flygut - An atlas of the Drosophila adult midgut

FBgn0086134

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004868

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Proteasome

MIST (genetic) - An integrated Molecular Interaction Database

41531

MIST (protein-protein) - An integrated Molecular Interaction Database

41531

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Regulation of ornithine decarboxylase (ODC)

References (101)

The following external sites may use different assemblies or annotations than FlyBase.

2.80

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