FlyBase Gene Report: Dmel\Prosβ5R2

General Information

Symbol

Dmel\Prosβ5R2

Species

D. melanogaster

Name

Proteasome β5 subunit-related 2

Annotation Symbol

CG31742

Feature Type

protein_coding_gene

FlyBase ID

FBgn0051742

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

36E3-36E3

Sequence location

2L:17,990,418..17,991,951 [+]

Recombination map

2-53

RefSeq locus

NT_033779 REGION:17990418..17991951

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables endopeptidase activity

inferred from sequence or structural similarity with SGD:S000006307

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000175708

(GO Reference Genome Project, 2011-)

enables threonine-type endopeptidase activity

inferred from electronic annotation with InterPro:IPR000243

(InterPro Project Members, 2004-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in proteasome-mediated ubiquitin-dependent protein catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN008521424

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with SGD:S000006307

(Gene Ontology Curators, 2002-)

involved_in proteolysis involved in protein catabolic process

inferred from electronic annotation with InterPro:IPR000243, InterPro:IPR001353, InterPro:IPR016050

(InterPro Project Members, 2004-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in cytosol

inferred from biological aspect of ancestor with PANTHER:PTN002613347

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN002613348

(GO Reference Genome Project, 2011-)

part_of proteasome core complex, beta-subunit complex

inferred from sequence or structural similarity with SGD:S000006307

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN002613347

(GO Reference Genome Project, 2011-)

part_of proteasome core complex

inferred from electronic annotation with InterPro:IPR000243, InterPro:IPR001353, InterPro:IPR016050

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

PROTEASOME ENDOPEPTIDASES

26S PROTEASOME - TESTIS-SPECIFIC VARIANT

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

PROTEASOME ENDOPEPTIDASES -: The proteasome is a threonine protease in which the active-site threonine residue is at the N-terminus of the beta subunits. Only three of the seven eukaryotic beta subunits (beta1, beta2 and beta5) retain an intact active site. These subunits are activated following autocatalytic processing of an N-terminal propeptide - propeptide autocleavage and substrate proteolysis utilize closely related mechanisms. (Adapted from PMID:28583440.)
26S PROTEASOME - TESTIS-SPECIFIC VARIANT -: The 26S proteasome degrades polyubiquitinated proteins in the cytoplasm and nucleus. It is formed from a cylindrical catalytic core 20S proteasome capped at each end by a regulatory 19S particle. The 19S regulatory particle recognizes, unfolds, and translocates ubiquitinated proteins into the catalytic core particle in an ATP-dependent manner. (Adapted from FBrf0215459).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Prosβ5R2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8INY4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

retrogene

(dos Santos, 2014, Zhang et al., 2011, Zhang et al., 2010, Pan and Zhang, 2009, Bai et al., 2007)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Prosβ5R2-RA

FBtr0081025

NM_165234

1082

279

Prosβ5R2-RB

FBtr0340183

NM_001299093

1534

279

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Prosβ5R2-PA

FBpp0080578

30.7

279

6.16

FBpp0309163

30.7

279

6.16

Q8INY4

NP_001286022

AHN54536

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

279 aa isoforms: Prosβ5R2-PA, Prosβ5R2-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q8INY4

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Prosβ5R2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Prosbeta5R2_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

4.95

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Prosβ5R2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Prosβ5R2

View exonic expression by tissue for Dmel\Prosβ5R2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

318924/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051742

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051742

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051742

Flygut - An atlas of the Drosophila adult midgut

FBgn0051742

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Prosβ5R2

Transgenic constructs containing regulatory region of Prosβ5R2

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel7070

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Prosβ5R2^GD10352

viable, with Scer\GAL4^Mef2.PR

Prosβ5R2^GD10352

Other Phenotypes

Allele

some die during pupal stage, with Scer\GAL4^pnr-MD237

Prosβ5R2^GD10352

visible, with Scer\GAL4^pnr-MD237

Prosβ5R2^GD10352

Phenotype manifest in

Allele

trichogen cell, with Scer\GAL4^pnr-MD237

Prosβ5R2^GD10352

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (19)

Hsap\PSMB5

10 of 14

Yes

Hsap\PSMB8

9 of 14

Hsap\PSMB11

5 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (16)

Rnor\Psmb5

10 of 14

Yes

Rnor\Psmb8

9 of 14

Rnor\Psmb11

4 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (17)

Mmus\Psmb5

9 of 14

Yes

Mmus\Psmb8

9 of 14

Yes

Mmus\Psmb11

5 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (8)

Xtro\psmb5

7 of 13

Yes

Xtro\LOC100485711

3 of 13

Yes

Xtro\psmb8

3 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (20)

Drer\psmb5

10 of 14

Yes

Drer\psmb8a

9 of 14

Drer\psmb11b

4 of 14

Yes

Drer\psmb11a

3 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (13)

Cele\pbs-5

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (14)

Agam\AgaP_AGAP010718

7 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (22)

Atha\AT3G26340

11 of 13

Yes

Atha\PBE1

11 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (14)

Scer\PRE2

11 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (12)

Spom\pts1

10 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (1)

Ecol\hslV

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Prosβ5R2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (24)

11 of 13

11 of 13

5 of 13

5 of 13

5 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PSMB5; proteasome 20S subunit beta 5

10 of 14

600306

PSMB8; proteasome 20S subunit beta 8

9 of 14

177046

PROTEASOME-ASSOCIATED AUTOINFLAMMATORY SYNDROME 1; PRAAS1

proteasome-associated autoinflammatory syndrome 1

PSMB11; proteasome subunit beta 11

5 of 14

611137

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

MIST Molecular Interaction Search Tool

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0051742

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Regulation of ornithine decarboxylase (ODC)

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Proteasome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-53

Cytogenetic map

36E3-36E3

Sequence location

2L:17,990,418..17,991,951 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

36E3-36E3

Limits computationally determined from genome sequence between P{lacW}Aac11^k06710 and P{EP}CG10413^EP2164

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-53

(FlyBase, 2016)

2-53.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

53392

y¹ w^*; Mi{MIC}Prosβ5R2^MI09388/SM6a

NIG-Fly

2LG-0317

y² VhaAC39-1^cho-1 v¹; P{U6-Prosβ5R2.NIG.sgRNA}attP40/CyO

VDRC

v108970

P{KK105262}VIE-260B

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (4)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

IP09987

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0051742

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0051742

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

New annotation (CG31742) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG31742 anon-AE003657.1

(FlyBase, 1996-)

Additional comments

The Prosβ5R2 gene may have been derived from the Prosβ5R1 gene by retroposition.

(Chen et al., 2012, Zhang et al., 2011, Pan and Zhang, 2009)

The Prosβ5R2 gene may have been derived from the Prosβ5R1 gene by retroposition sometime after the divergence of the Sophophora and Drosophila subgenera.

(Zhang et al., 2010)

The CG31742 gene may have been derived from the Prosβ5R gene by retroposition.

(Bai et al., 2007)

Source for merge of CG31742 anon-AE003657.1 was sequence comparison ( date:030626 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Prosβ5R2 CG31742

(FlyBase, 2009-)

Nomenclature comments

The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms.

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

31742

(Ravi et al., 2009)

CG31742

(National Institute of Genetics Fly Stocks, 2022-, Kumimoto et al., 2013, Schertel et al., 2013, Chen et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Zhang et al., 2011, Zhang et al., 2011, Zhang et al., 2010, Zhang et al., 2010, Belote and Zhong, 2009, Keleman et al., 2009.8.5, Pan and Zhang, 2009, Pan and Zhang, 2009, Bai et al., 2007, Bai et al., 2007)

Prosbeta5R2

(National Institute of Genetics Fly Stocks, 2022-, Cardoso-Moreira et al., 2016, dos Santos, 2014)

Prosβ5R2

anon-AE003657.1

β5R2

(Belote and Zhong, 2009)

Name Synonyms

AE003657 - proteasome beta subunit

(Rawlings, 2001.2.12)

Proteasome β5 subunit-related 2

Secondary FlyBase IDs

FBgn0043961

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 73 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

318924

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8INY4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q8INY4

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8INY4

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

318924/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051742

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051742

FlyMine - An integrated database for Drosophila genomics

FBgn0051742

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG31742

MARRVEL_MODEL - MARRVEL (model organism gene)

318924

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0051742

DroID - A comprehensive database of gene and protein interactions.

FBgn0051742

DRSC - Results frm RNAi screens

FBgn0051742

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Prosbeta5R2_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051742

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0051742

Flygut - An atlas of the Drosophila adult midgut

FBgn0051742

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0051742

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Proteasome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Regulation of ornithine decarboxylase (ODC)

References (37)

The following external sites may use different assemblies or annotations than FlyBase.

4.95

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