FB2024_03 , released June 25, 2024
Allele: Dmel\Zip89BUAS.Tag:FLAG
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General Information
Symbol
Dmel\Zip89BUAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0288197
Feature type
allele
Associated gene
Dmel\Zip89B
Associated Insertion(s)
Carried in Construct
M{UAS-Zip89B.flag}
Also Known As
dZip89Bflag, pUAST dZip89Bflag
Key Links
Transgenic product class
wild_type
(Lye et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Lye et al., 2012)
Mutagen
in vitro construct
(Lye et al., 2012)
Progenitor genotype
Carried in construct
M{UAS-Zip89B.flag}
Cytology
Description

UASt regulatory sequences drive expression of Zip89B which is tagged at the C-terminal end with Tag:FLAG.

(Lye et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Zip89B
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4mex1.2.1 does not significantly affect survival rate as compared with wild type when flies are raised from first instar larval stage to adulthood on zinc-supplemented media (12mmol/L ZnCl[[2]]).

      Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4da.G32 results in a significant increase in survival as compared to wild type when flies are raised from first instar larval stage to adulthood on media supplemented with TPEN.

      Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Zip89B.PR does not result in any obvious phenotypes or alter the survival of flies on any zinc-modulated diets.

      Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.long results in a mild rough eye phenotype, and expression of two copies of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.long results in a severe rough eye phenotype.

      (Richards et al., 2015)

      Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4tub.PU results in delayed development.

      Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PFa does not result in a mutant phenotype.

      Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4pnr-MD237 does not result in a mutant phenotype.

      (Lye et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Enhancer of
      Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Enhancer of
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The adult eye phenotype seen upon expression of ZnT86DScer\UAS.cDa under the control of Scer\GAL4GMR.long is exacerbated by co-expression of Zip89BScer\UAS.T:Zzzz\FLAG : the morphological defects strongly so while the pigmentation loss is only mildly worsened. Co-expression of Zip89BScer\UAS.T:Zzzz\FLAG also leads to partial lethality.

      The morphological and pigmentation defects in the adult eye seen upon expression of ZnT86DScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.long are strongly ameliorated by co-expression of Zip89BScer\UAS.T:Zzzz\FLAG.

      The morphological and pigmentation defects in the adult eye seen upon expression of Zip71BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.long are exacerbated by co-expression of Zip89BScer\UAS.T:Zzzz\FLAG.

      (Dechen et al., 2015)

      Co-expression of foiScer\UAS.T:Zzzz\FLAG or ZnT41FVDRC.cUa moderately exacerbates the rough eye phenotype of flies expressing Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.long.

      Co-expression of Zip42C.1Scer\UAS.T:Zzzz\FLAG, Zip42C.1Scer\UAS.T:Avic\GFP-EGFP, Zip42C.2Scer\UAS.T:Zzzz\FLAG or ZnT63CVDRC.cUa severely exacerbates the rough eye phenotype of flies expressing Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.long.

      Co-expression of Zip89BScer\UAS.T:Zzzz\FLAG partially rescues eye phenotypes of flies expressing ZnT86DScer\UAS.T:Disc\RFP-mCherry or ZnT86DScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.long.

      (Richards et al., 2015)

      Co-expression of Zip89BScer\UAS.T:Zzzz\FLAG and Zip42C.1Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PFa results in a flattened eye.

      Co-expression of Zip89BScer\UAS.T:Zzzz\FLAG and Zip42C.1Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4pnr-MD237 results in an abnormal thoracic bristle alignment.

      (Lye et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Zip89BUAS.Tag:FLAG/Scer\GAL4mex1.2.1 rescues Zip89BΔ

      (Richards et al., 2015)
      Comments

      Expression of Zip89BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4mex1.2.1 fully restores survival of Zip89BΔ/Zip89BΔ flies on high-zinc media back to wild type levels.

      (Richards et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Zip89BScer\UAS.T:Zzzz\FLAG
      Zip89BUAS.Tag:FLAG
      dZIP89BFLAG
      (Richards et al., 2015)
      dZip89Bflag
      (Lye et al., 2013)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)