FlyBase Allele Report: Dmel\Vps35[MH20]

General Information

Symbol

Dmel\Vps35^MH20

Species

D. melanogaster

Name

FlyBase ID

FBal0221635

Feature type

allele

Associated gene

Dmel\Vps35

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Franch-Marro et al., 2008)

Mutagen

P-element activity

(Franch-Marro et al., 2008)

Progenitor genotype

P{EPgy2}Vps35^EY14200

(Franch-Marro et al., 2008)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion of nearly 2kb, which removes the first three exons of Vps35, including the translation start site.

(Franch-Marro et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA with Vps35^E42

(Malik et al., 2015)

CEA with park²⁵

(Malik et al., 2015)

DOES NOT model Parkinson's disease

CEA with Pink1^B9

(Malik et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease 8

modeled by Lrrk¹

(Inoshita et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Parkinson's disease-related phenotypes are observed in Vps35^E42 park²⁵ double heterozygotes.. No Parkinson's disease-related phenotypes are observed in Vps35^MH20/Pink1^B9 double heterozygotes.

(Malik et al., 2015)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian behavior | adult stage | dominant

(Inoshita et al., 2017)

abnormal developmental rate | pupal stage | somatic clone

(Strutt et al., 2019)

abnormal gravitaxis (with Vps35^E42), with Scer\GAL4^da.PU, Vps35^UAS.D650N

(Malik et al., 2015)

abnormal gravitaxis (with Vps35^E42), with Scer\GAL4^da.PU, Vps35^UAS.R550W

(Malik et al., 2015)

abnormal locomotor behavior | dominant | third instar larval stage

(Inoshita et al., 2017)

abnormal locomotor behavior | larval stage (with Vps35^E42)

(Malik et al., 2015)

abnormal neuroanatomy | dominant | third instar larval stage

(Inoshita et al., 2017)

abnormal neurophysiology

(Wang et al., 2014)

abnormal neurophysiology | adult stage

(Ye et al., 2020)

abnormal neurophysiology | adult stage | dominant

(Inoshita et al., 2017)

abnormal neurophysiology | adult stage | progressive | somatic clone

(Ye et al., 2020)

abnormal neurophysiology | dominant | third instar larval stage

(Inoshita et al., 2017)

abnormal neurophysiology | third instar larval stage (with Vps35¹)

(Inoshita et al., 2017)

abnormal planar polarity | pupal stage | somatic clone

(Strutt et al., 2019)

abnormal size | third instar larval stage (with Vps35¹)

(Inoshita et al., 2017)

lethal (with Df(2R)Exel6078)

(Franch-Marro et al., 2008)

lethal | embryonic stage | maternal effect | recessive

(Franch-Marro et al., 2008)

lethal | prepupal stage | recessive

(Franch-Marro et al., 2008)

lethal - all die before end of prepupal stage (with Vps35¹)

(Inoshita et al., 2017)

melanotic mass phenotype | larval stage

(Franch-Marro et al., 2008)

melanotic mass phenotype | larval stage (with Df(2R)Exel6078)

(Franch-Marro et al., 2008)

short lived | dominant

(Inoshita et al., 2017)

visible | somatic clone

(Franch-Marro et al., 2008)

Phenotype Manifest In

dendritic arborizing neuron | adult stage

(Inoshita et al., 2017)

embryonic/larval neuromuscular junction | third instar larval stage (with Vps35¹)

(Inoshita et al., 2017)

endosome | progressive

(Wang et al., 2014)

follicle cell | somatic clone

(Pocha et al., 2011)

lysosome | progressive

(Wang et al., 2014)

melanotic mass | larval stage

(Franch-Marro et al., 2008)

NMJ bouton | increased number (with Vps35^E42)

(Malik et al., 2015)

photoreceptor neuron

(Wang et al., 2014)

retina | adult stage

(Ye et al., 2020)

retina | adult stage | somatic clone

(Ye et al., 2020)

subsynaptic reticulum | third instar larval stage (with Vps35¹)

(Inoshita et al., 2017)

synaptic vesicle | third instar larval stage (with Vps35¹)

(Inoshita et al., 2017)

terminal bouton | third instar larval stage (with Vps35¹)

(Inoshita et al., 2017)

type I terminal bouton | third instar larval stage

(Inoshita et al., 2017)

type I terminal bouton | third instar larval stage (with Vps35¹)

(Inoshita et al., 2017)

wing | pupal stage | somatic clone

(Strutt et al., 2019)

wing hair | pupal stage | somatic clone

(Strutt et al., 2019)

wing margin | somatic clone

(Franch-Marro et al., 2008)

Detailed Description

Statement

Reference

Electroretinogram readings in Vps35^MH20 homozygotes show poorly sustained transient potentials; in full-eye Vps35^MH20 clones, there is disrupted light-induced depolarization, with progressively decreased amplitudes and on/off transients.

(Ye et al., 2020)

Vps35^MH20 clones in the pupal wing present planar cell polarity defects, as shown by decreased core protein asymmetry and decreased planar polarity coordination (Pk and Fmi proteins), as well as a delay in wing trichome initiation, as compared to controls.

(Strutt et al., 2019)

Vps35^MH20/Vps35¹ transheterozygotes are lethal at the prepupal stages. Their third instar larval neuromuscular junction exhibit a significant decrease in the size of synaptic boutons and reciprocal increase in the number of type Ib boutons (despite of insignificant changes in the number of active zones per bouton), a significant decrease in the synaptic pool, associated with the reciprocal significant decrease in number and increase in size of synaptic vesicles at active zones, a significant decrease in endocytosis, associated with a significant increase in the number of endocytic intermediates per active zone, and subtle ultrastructural defects at the subsynaptic reticulum, as compared to controls; these neuromuscular junctions also exhibit neurotransmission defects, namely a significant increase in miniature excitatory junction potential amplitude, a significant decrease in quantal content under low stimulation conditions, despite of insignificant changes in both excitatory junction potential amplitude and in paired-pulse ratio, and a significant decrease in excitatory junction potential amplitude under high stimulation conditions, as compared to controls.

Vps35^MH20 heterozygous third instar larvae exhibit a significant decrease in locomotor activity. Their neuromuscular junctions exhibit a significant increase in the number of type Ib synaptic boutons and a significant decrease in excitatory junction potential amplitude under high stimulation conditions, as compared to wild-type controls. Adult heterozygotes exhibit a significant decrease in lifespan, a defective sleep behavior, shown by a reciprocal significant increase in sleep bouts during the light period and a significant decrease in sleep bouts during the dark period, and a significant decrease in spontaneous synaptic activity by adult dendritic arborizing neuron, as compared to controls.

(Inoshita et al., 2017)

Vps35^MH20 mutant third instar larvae do not show lysosomal expansion phenotype.

(Bharadwaj et al., 2016)

Vps35^MH20/Vps35^E42 mutants show supernumerary boutons and a dramatic reduction in larval locomotion.

Flies expressing Vps35^Scer\UAS.cMa under the control of Scer\GAL4^da.PU in a Vps35^MH20/Vps35^E42 mutant background show similar climbing ability to controls.

Flies expressing Vps35^{Scer\UAS.D650N} under the control of Scer\GAL4^da.PU in a Vps35^MH20/Vps35^E42 mutant background show climbing defects.

Flies expressing Vps35^{Scer\UAS.L800M} under the control of Scer\GAL4^da.PU in a Vps35^MH20/Vps35^E42 mutant background show similar climbing ability to controls.

Flies expressing Vps35^{Scer\UAS.R550W} under the control of Scer\GAL4^da.PU in a Vps35^MH20/Vps35^E42 mutant background show climbing defects.

(Malik et al., 2015)

Mutant clones of Vps35^MH20 in eye discs exhibit a nearly complete loss of Vps26 signalling and a subsequent progressive loss of ERG amplitudes compared to controls and some morphological defects in aged photoreceptor cells.

Vps35^MH20 flies raised in a light/dark cycle for 4 days show obvious ultrastructural defects in photoreceptor cells when compared to controls. These defects include a dramatic increase in late endosomes and lysosomes. However, photoreceptor cells in dark-reared Vps35^MH20 flies do not show an increased number of late endosomes or lysosomes when compared to controls.

(Wang et al., 2014)

The follicular epithelium loses its monolayer structures and appears multilayered in 19% of homozygous follicle cell clones between stages 7 and 10 of oogenesis.

(Pocha et al., 2011)

Homozygous larvae develop melanotic tumours during late larval stages and die as prepupae.

Mutant embryos derived from homozygous female germline clones die at the end of embryogenesis.

Homozygous clones in the wing result in wing notching.

(Franch-Marro et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Vps35^MH20 has abnormal neurophysiology | third instar larval stage | dominant phenotype, enhanceable by Rab5^UAS.GFP/Scer\GAL4^Toll-6-D42

(Inoshita et al., 2017)

Suppressed by

Statement

Reference

Vps35^MH20/Vps35¹ has abnormal size | third instar larval stage phenotype, suppressible by Rab5^UAS.GFP/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has abnormal size | third instar larval stage phenotype, suppressible by Rab11^{UAS.ORF.Tag:HA}/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has abnormal size | third instar larval stage phenotype, suppressible by Lrrk^UAS.cIa/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has abnormal size | third instar larval stage phenotype, suppressible by Lrrk[+]/Lrrk^e03680

(Inoshita et al., 2017)

Vps35^MH20 has abnormal neuroanatomy | dominant | third instar larval stage phenotype, suppressible by Lrrk[+]/Lrrk^e03680

(Inoshita et al., 2017)

Vps35^MH20 has abnormal neuroanatomy | dominant | third instar larval stage phenotype, suppressible by Lrrk^UAS.cIa/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20 has abnormal neurophysiology | dominant | third instar larval stage phenotype, suppressible by Lrrk[+]/Lrrk¹

(Inoshita et al., 2017)

Vps35^MH20 has abnormal neurophysiology | dominant | third instar larval stage phenotype, suppressible by Lrrk^UAS.cIa/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20 has abnormal neurophysiology | dominant | third instar larval stage phenotype, suppressible by Scer\GAL4^Toll-6-D42/EndoA^UAS.cVa

(Inoshita et al., 2017)

Vps35^MH20 has abnormal neurophysiology | dominant | third instar larval stage phenotype, suppressible by Rab11^{UAS.ORF.Tag:HA}/Scer\GAL4^Toll-6-D42

(Inoshita et al., 2017)

Vps35^MH20 has abnormal neurophysiology | dominant | third instar larval stage phenotype, suppressible | partially by Rab5^GD10492/Scer\GAL4^Toll-6-D42

(Inoshita et al., 2017)

Vps35^MH20 has abnormal locomotor behavior | dominant | third instar larval stage phenotype, suppressible by Lrrk^UAS.cIa/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20 has abnormal locomotor behavior | dominant | third instar larval stage phenotype, suppressible by Lrrk[+]/Lrrk¹

(Inoshita et al., 2017)

Vps35^MH20 has abnormal locomotor behavior | dominant | third instar larval stage phenotype, suppressible by Rab5^UAS.GFP/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20 has abnormal locomotor behavior | dominant | third instar larval stage phenotype, suppressible by Rab11^{UAS.ORF.Tag:HA}/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35^E42 has abnormal locomotor behavior | larval stage phenotype, suppressible | partially by Scer\GAL4^da.PU/Hsap\VPS35^R524W.UAS

(Malik et al., 2015)

Vps35^MH20/Vps35^E42 has abnormal locomotor behavior | larval stage phenotype, suppressible | partially by Scer\GAL4^da.PU/Hsap\VPS35^L774M.UAS

(Malik et al., 2015)

Vps35^MH20/Vps35^E42 has abnormal locomotor behavior | larval stage phenotype, suppressible by Scer\GAL4^da.PU/Hsap\VPS35^UAS.cMa

(Malik et al., 2015)

Vps35^MH20/Vps35^E42 has abnormal locomotor behavior | larval stage phenotype, suppressible | partially by Scer\GAL4^da.PU/Hsap\VPS35^D620N.UAS

(Malik et al., 2015)

NOT suppressed by

Statement

Reference

Vps35^MH20/Vps35¹ has lethal - all die before end of prepupal stage phenotype, non-suppressible by Lrrk^e03680/Lrrk^e03680

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has lethal - all die before end of prepupal stage phenotype, non-suppressible by Hsap\VPS35^UAS.cIa/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has lethal - all die before end of prepupal stage phenotype, non-suppressible by Scer\GAL4^da.G32/Hsap\VPS35^{D620N.UAS.cIa}

(Inoshita et al., 2017)

Vps35^MH20 has abnormal neurophysiology | dominant | third instar larval stage phenotype, non-suppressible by EndoA^Δ4/EndoA[+]

(Inoshita et al., 2017)

Vps35^MH20/Vps35^E42 has lethal phenotype, non-suppressible by Scer\GAL4^da.PU/Hsap\VPS35^UAS.cMa

(Malik et al., 2015)

Vps35^MH20/Vps35^E42 has lethal phenotype, non-suppressible by Scer\GAL4^da.PU/Hsap\VPS35^D620N.UAS

(Malik et al., 2015)

Vps35^MH20/Vps35^E42 has lethal phenotype, non-suppressible by Scer\GAL4^da.PU/Hsap\VPS35^R524W.UAS

(Malik et al., 2015)

Vps35^MH20/Vps35^E42 has lethal phenotype, non-suppressible by Scer\GAL4^da.PU/Hsap\VPS35^L774M.UAS

(Malik et al., 2015)

Suppressor of

Statement

Reference

Vps35^MH20/Vps35[+] is a suppressor of abnormal neuroanatomy | dominant | third instar larval stage phenotype of Lrrk^e03680

(Inoshita et al., 2017)

Vps35^MH20/Vps35[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Lrrk^UAS.cIa, Scer\GAL4^da.G32

(Inoshita et al., 2017)

Other

Statement

Reference

Vps35^MH20, park[+]/park²⁵ has abnormal oxidative stress response phenotype

(Malik et al., 2015)

Vps35^MH20, park[+]/park²⁵ has abnormal neuroanatomy | progressive phenotype

(Malik et al., 2015)

Vps35^MH20, park[+]/park²⁵ has abnormal locomotor behavior | adult stage | progressive phenotype

(Malik et al., 2015)

Hsap\VPS35^UAS.cMa, Scer\GAL4^da.PU, Vps35^MH20/Vps35^E42 has majority die during pupal stage phenotype

(Malik et al., 2015)

Hsap\VPS35^D620N.UAS, Scer\GAL4^da.PU, Vps35^MH20/Vps35^E42 has majority die during pupal stage phenotype

(Malik et al., 2015)

Hsap\VPS35^R524W.UAS, Scer\GAL4^da.PU, Vps35^MH20/Vps35^E42 has majority die during pupal stage phenotype

(Malik et al., 2015)

Hsap\VPS35^L774M.UAS, Scer\GAL4^da.PU, Vps35^MH20/Vps35^E42 has majority die during pupal stage phenotype

(Malik et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Vps35^MH20/Vps35¹ has type I terminal bouton | third instar larval stage phenotype, suppressible by Rab5^UAS.GFP/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has type I terminal bouton | third instar larval stage phenotype, suppressible by Rab11^{UAS.ORF.Tag:HA}/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has type I terminal bouton | third instar larval stage phenotype, suppressible by Lrrk^UAS.cIa/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has type I terminal bouton | third instar larval stage phenotype, suppressible by Lrrk[+]/Lrrk^e03680

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has synaptic vesicle | third instar larval stage phenotype, suppressible by Rab5^UAS.GFP/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has synaptic vesicle | third instar larval stage phenotype, suppressible by Rab11^{UAS.ORF.Tag:HA}/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has synaptic vesicle | third instar larval stage phenotype, suppressible by Lrrk^UAS.cIa/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has synaptic vesicle | third instar larval stage phenotype, suppressible by Lrrk[+]/Lrrk^e03680

(Inoshita et al., 2017)

Vps35^MH20 has type I bouton | third instar larval stage phenotype, suppressible by Lrrk[+]/Lrrk^e03680

(Inoshita et al., 2017)

Vps35^MH20 has type I bouton | third instar larval stage phenotype, suppressible by Lrrk^UAS.cIa/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Suppressor of

Statement

Reference

Vps35^MH20/Vps35[+] is a suppressor of type I bouton | third instar larval stage phenotype of Lrrk^e03680

(Inoshita et al., 2017)

Vps35^MH20/Vps35[+] is a suppressor of type I bouton | third instar larval stage phenotype of Lrrk^UAS.cIa, Scer\GAL4^da.G32

(Inoshita et al., 2017)

Other

Statement

Reference

Vps35^MH20, park[+]/park²⁵ has dopaminergic PPL1 neuron | progressive phenotype

(Malik et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The prepupal lethality of Vps35^MH20/Vps35¹ transheterozygotes is not rescued by Lrrk^e03680 homozygosity.

In the third instar larval neuromuscular junctions of Vps35^MH20/Vps35¹ transheterozygotes, their reciprocal increased size and decreased number of synaptic vesicles observed are suppressed either by Lrrk^e03680 heterozygosity or by the expression of Rab5^{Scer\UAS.T:Avic\GFP}, Rab11^{Scer\UAS.ORF.T:Ivir\HA1} or Lrrk[Scer\UAS.cIa] under the control of Scer\GAL4^da.G32; their increased number of type Ib boutons and reciprocal decreased bouton size are also suppressed by the expression of either Rab5^{Scer\UAS.T:Avic\GFP} or Rab11^{Scer\UAS.ORF.T:Ivir\HA1} under the control of Scer\GAL4^da.G32.

The decreased locomotor activity of Vps35^MH20 heterozygous third instar larvae is suppressed either by the expression of Lrrk^Scer\UAS.cIa, Rab5^{Scer\UAS.T:Avic\GFP} or Rab11^{Scer\UAS.ORF.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 or by Lrrk¹ heterozygosity. In the neuromuscular junction of these heterozygous larvae, the decreased excitatory junction potential amplitude under high stimulation conditions is fully suppressed by the expression of Lrrk^Scer\UAS.cIa under the control of Scer\GAL4^da.G32, by the expression of EndoA^Scer\UAS.cVa or Rab11^{Scer\UAS.ORF.T:Ivir\HA1} under the control of Scer\GAL4^Toll-6-D42, or by Lrrk¹ heterozygosity, is partially suppressed by the expression of Rab5^GD10492 under the control of Scer\GAL4^Toll-6-D42, is enhanced by the expression of Rab5^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Toll-6-D42, and is unaffected by EndoA^Δ4 heterozygosity.

Double heterozygosity for Vps35^MH20 and Lrrk^e03680 or the expression of Lrrk^Scer\UAS.cIa under the control of Scer\GAL4^da.G32 in a Vps35^MH20 heterozygous background suppresses the increased number of type Ib synaptic boutons observed in the third instar larval neuromuscular junction of Vps35^MH20 or Lrrk^e03680 heterozygotes, or upon expression of Lrrk^Scer\UAS.cIa under the control of Scer\GAL4^da.G32.

(Inoshita et al., 2017)

Vps35^MH20 park²⁵ double heterozygotes show an age-dependent reduction in climbing ability. No phenotypes are observed in either heterozygote alone.

Vps35^MH20 park²⁵ double heterozygotes show increased sensitivity to the oxidative stressor paraquat compared with either heterozygote alone, similar to that seen in park²⁵ homozygotes. In contrast to either heterozygote alone, progressive degeneration of the dopaminergic neurons in the PPPL cluster is also seen in Vps35^MH20 park²⁵ double heterozygotes.

Vps35^MH20 Pink1^B9 double heterozygotes do not exhibit climbing defects.

(Malik et al., 2015)

Xenogenetic Interactions

Statement

Reference

The prepupal lethality of Vps35^MH20/Vps35¹ transheterozygotes is not rescued by the Scer\GAL4^da.G32-driven expression of Hsap\VPS35^Scer\UAS.cIa or Hsap\VPS35^{D620N.Scer\UAS.cIa}.

(Inoshita et al., 2017)

Expression of Hsap\VPS35^Scer\UAS.cMa under the control of Scer\GAL4^da.PU at 25[o] fails to suppress the phenotypes seen in Vps35^MH20/Vps35^E42 mutant flies. Expressing Hsap\VPS35^Scer\UAS.cMa at 29[o]C rescues mutant viability to the late pupal stage, but adults fail to eclose.

Expression of Hsap\VPS35^Scer\UAS.cMa under the control of Scer\GAL4^da.PU suppresses the larval locomotion defects seen in Vps35^MH20/Vps35^E42 mutants.

Expression of Hsap\VPS35^Scer\UAS.cMa under the control of Scer\GAL4^da.PU at 29[o] suppresses the NMJ phenotypes seen in Vps35^MH20/Vps35^E42 mutant larvae.

Expression of Hsap\VPS35^{D620N.Scer\UAS} under the control of Scer\GAL4^da.PU at 25[o] fails to suppress the phenotypes seen in Vps35^MH20/Vps35^E42 mutant flies. Expressing Hsap\VPS35^{D620N.Scer\UAS} at 29[o]C rescues mutant viability to the late pupal stage, but adults fail to eclose.

Expression of Hsap\VPS35^{D620N.Scer\UAS} under the control of Scer\GAL4^da.PU at 29[o] suppresses the NMJ phenotypes seen in Vps35^MH20/Vps35^E42 mutant larvae.

Expression of Hsap\VPS35^{D620N.Scer\UAS} under the control of Scer\GAL4^da.PU partially suppresses the larval locomotion defects seen in Vps35^MH20/Vps35^E42 mutants.

Expression of Hsap\VPS35^{R524W.Scer\UAS} under the control of Scer\GAL4^da.PU at 25[o] fails to suppress the phenotypes seen in Vps35^MH20/Vps35^E42 mutant flies. Expressing Hsap\VPS35^{R524W.Scer\UAS} at 29[o]C rescues mutant viability to the late pupal stage, but adults fail to eclose.

Expression of Hsap\VPS35^{R524W.Scer\UAS} under the control of Scer\GAL4^da.PU partially suppresses the larval locomotion defects seen in Vps35^MH20/Vps35^E42 mutants.

Expression of Hsap\VPS35^{R524W.Scer\UAS} under the control of Scer\GAL4^da.PU at 29[o] suppresses the NMJ phenotypes seen in Vps35^MH20/Vps35^E42 mutant larvae.

Expression of Hsap\VPS35^{L774M.Scer\UAS} under the control of Scer\GAL4^da.PU at 25[o] fails to suppress the phenotypes seen in Vps35^MH20/Vps35^E42 mutant flies. Expressing Hsap\VPS35^{L774M.Scer\UAS} at 29[o]C rescues mutant viability to the late pupal stage, but adults fail to eclose.

Expression of Hsap\VPS35^{L774M.Scer\UAS} under the control of Scer\GAL4^da.PU at 29[o] suppresses the NMJ phenotypes seen in Vps35^MH20/Vps35^E42 mutant larvae.

Expression of Hsap\VPS35^{L774M.Scer\UAS} under the control of Scer\GAL4^da.PU partially suppresses the larval locomotion defects seen in Vps35^MH20/Vps35^E42 mutants.

(Malik et al., 2015)

Complementation and Rescue Data

Rescued by

Vps35^MH20/Vps35¹ is rescued by Scer\GAL4^da.G32/Vps35^UAS.cIa

(Inoshita et al., 2017)

Vps35^MH20/Vps35^E42 is rescued by Scer\GAL4^da.PU/Vps35^UAS.cMa

(Malik et al., 2015)

Partially rescued by

Vps35^MH20/Vps35¹ is partially rescued by Vps35^D647N.UAS/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35^E42 is partially rescued by Scer\GAL4^da.PU/Vps35^UAS.D650N

(Malik et al., 2015)

Vps35^MH20/Vps35^E42 is partially rescued by Scer\GAL4^da.PU/Vps35^UAS.R550W

(Malik et al., 2015)

Vps35^MH20/Vps35^E42 is partially rescued by Vps35^UAS.L800M/Scer\GAL4^da.PU

(Malik et al., 2015)

Comments

The prepupal lethality of Vps35^MH20/Vps35¹ transheterozygotes is rescued by the Scer\GAL4^da.G32-driven expression of Vps35^Scer\UAS.cIa, but not of Vps35^{D647N.Scer\UAS}.

In the third instar larval neuromuscular junction of Vps35^MH20/Vps35¹ transheterozygotes, the decreased size of synaptic boutons is rescued by the expression of either Vps35^Scer\UAS.cIa or Vps35^{D647N.Scer\UAS} under the control of Scer\GAL4^da.G32; the decreased pool of synaptic vesicles, the associated reciprocal increased number and decreased size of synaptic boutons, the decreased endocytosis and the subtle ultrastructural defects of the subsynaptic reticulum are rescued by the expression of Vps35^Scer\UAS.cIa, but not Vps35^{D647N.Scer\UAS}, under the control of Scer\GAL4^da.G32; the increased miniature excitatory junction potential amplitude and the decreased excitatory junction potential amplitude under high stimulation conditions are rescued by the expression of Vps35^Scer\UAS.cIa, but not Vps35^{D647N.Scer\UAS}, under the control of either Scer\GAL4^da.G32, Scer\GAL4^Toll-6-D42 or Scer\GAL4^Mhc.PK.

The decreased adult lifespan, the decreased spontaneous synaptic activity of adult dendritic arborizing neurons and the increased number of type Ib synaptic boutons at the third instar larval neuromuscular junction observed in Vps35^MH20 heterozygotes are suppressed by the expression of Vps35^Scer\UAS.cIa, but not of Vps35^{D647N.Scer\UAS}, under the control of Scer\GAL4^ple.PF (driver used in the dendritic arborizing neuron activity rescue experiment) or Scer\GAL4^da.G32 (driver used in the other experiments).

(Inoshita et al., 2017)

Expression of Vps35^Scer\UAS.cMa under the control of Scer\GAL4^da.PU at 25[o] fails to rescue the phenotypes seen in Vps35^MH20/Vps35^E42 mutant flies. Expressing Vps35^Scer\UAS.cMa at 29[o]C prevents the appearance of melanotic masses and fully rescues the developmental lethality, with progeny appearing in expected proportions.

Expression of Vps35^Scer\UAS.cMa under the control of Scer\GAL4^da.PU at 29[o] rescues the NMJ phenotypes seen in Vps35^MH20/Vps35^E42 mutant larvae.

Expression of Vps35^Scer\UAS.cMa under the control of Scer\GAL4^da.PU rescues the larval locomotion defects seen in Vps35^MH20/Vps35^E42 mutants.

Expression of Vps35^{Scer\UAS.D650N} under the control of Scer\GAL4^da.PU at 25[o] fails to rescue the phenotypes seen in Vps35^MH20/Vps35^E42 mutant flies. Expressing Vps35^{Scer\UAS.D650N} at 29[o]C prevents the appearance of melanotic masses and fully rescues the developmental lethality, with progeny appearing in expected proportions.

Expression of Vps35^{Scer\UAS.D650N} under the control of Scer\GAL4^da.PU partially rescues the larval locomotion defects seen in Vps35^MH20/Vps35^E42 mutants.

Expression of Vps35^{Scer\UAS.D650N} under the control of Scer\GAL4^da.PU at 29[o] rescues the NMJ phenotypes seen in Vps35^MH20/Vps35^E42 mutant larvae.

Expression of Vps35^{Scer\UAS.L800M} under the control of Scer\GAL4^da.PU at 25[o] fails to rescue the phenotypes seen in Vps35^MH20/Vps35^E42 mutant flies. Expressing Vps35^{Scer\UAS.L800M} at 29[o]C prevents the appearance of melanotic masses and fully rescues the developmental lethality, with progeny appearing in expected proportions.

Expression of Vps35^{Scer\UAS.L800M} under the control of Scer\GAL4^da.PU at 29[o] rescues the NMJ phenotypes seen in Vps35^MH20/Vps35^E42 mutant larvae.

Expression of Vps35^{Scer\UAS.L800M} under the control of Scer\GAL4^da.PU partially rescues the larval locomotion defects seen in Vps35^MH20/Vps35^E42 mutants.

Expression of Vps35^{Scer\UAS.R550W} under the control of Scer\GAL4^da.PU at 25[o] fails to rescue the phenotypes seen in Vps35^MH20/Vps35^E42 mutant flies. Expressing Vps35^{Scer\UAS.R550W} at 29[o]C prevents the appearance of melanotic masses and fully rescues the developmental lethality, with progeny appearing in expected proportions.

Expression of Vps35^{Scer\UAS.R550W} under the control of Scer\GAL4^da.PU at 29[o] rescues the NMJ phenotypes seen in Vps35^MH20/Vps35^E42 mutant larvae.

Expression of Vps35^{Scer\UAS.R550W} under the control of Scer\GAL4^da.PU partially rescues the larval locomotion defects seen in Vps35^MH20/Vps35^E42 mutants.

(Malik et al., 2015)

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Mutant

Wild-type

Stocks (1)

Bloomington

67202

w^*; P{neoFRT}42D Vps35^MH20/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Vps35^HM20

(Yamashita et al., 2022)

Vps35^MH20

(Zhou et al., 2023, Inoshita et al., 2022, Laiouar et al., 2020, Pannen et al., 2020, Ye et al., 2020, Strutt et al., 2019, Lin et al., 2018, Gomez-Lamarca et al., 2015, Malik et al., 2015, Wang et al., 2014, Pocha et al., 2011, Franch-Marro et al., 2008)

dVps35^MH20

(Inoshita et al., 2017)

vps35^MH20

(Bharadwaj et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (15)

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