FB2024_03 , released June 25, 2024
Allele: Dmel\Vps35UAS.cIa
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General Information
Symbol
Dmel\Vps35UAS.cIa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Inoshita
FlyBase ID
FBal0335422
Feature type
allele
Associated gene
Dmel\Vps35
Associated Insertion(s)
Carried in Construct
P{UAS-Vps35.I}
Key Links
Transgenic product class
cDNA
(Inoshita et al., 2017)
wild_type
(Inoshita et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Inoshita et al., 2017)
wild_type
(Inoshita et al., 2017)
Mutagen
in vitro construct
(Inoshita et al., 2017)
Progenitor genotype
Carried in construct
P{UAS-Vps35.I}
Cytology
Description

UASt regulatory sequences drive expression of wild-type Vps35 cDNA.

(Inoshita et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Vps35
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Parkinson's disease 8
      modeled by Lrrk1
      (Inoshita et al., 2022)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Vps35Scer\UAS.cIa under the control of Scer\GAL4da.G32 in a Vps351/Vps35MH20 transheterozygous background does not affect the number of active zones in third instar larval neuromuscular junction boutons, as compared to controls.

      (Inoshita et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4da.G32/Vps35UAS.cIa rescues Vps35MH20/Vps351

      (Inoshita et al., 2017)
      Comments

      The expression of Vps35Scer\UAS.cIa under the control of Scer\GAL4da.G32 rescues the prepupal lethality of Vps351/Vps35MH20.

      In the third instar larval neuromuscular junction of Vps351/Vps35MH20 transheterozygotes, the decreased pool of synaptic vesicles, the associated reciprocal increased number and increased size of synaptic boutons, the decreased endocytosis and the subtle ultrastructural defects of the subsynaptic reticulum are rescued by the expression of Vps35Scer\UAS.cIa under the control of Scer\GAL4da.G32; the increased miniature excitatory junction potential amplitude and the decreased excitatory junction potential amplitude under high stimulation conditions are rescued by the expression of Vps35Scer\UAS.cIa, but not Vps35D647N.Scer\UAS, under the control of either Scer\GAL4da.G32, Scer\GAL4Toll-6-D42 or Scer\GAL4Mhc.PK.

      The expression of Vps35Scer\UAS.cIa under the control of Scer\GAL4ple.PF rescues the decreased sleep bouts displayed by Vps351 heterozygous adults.

      In Vps35MH20 heterozygotes, the expression of Vps35Scer\UAS.cIa under the control of Scer\GAL4da.G32 rescues the decreased adult lifespan and the increased number of type Ib synaptic boutons in third instar larval neuromuscular junction; the expression of Vps35Scer\UAS.cIa under the control of Scer\GAL4ple.PF rescues the decreased spontaneous synaptic activity of adult dendritic arborizing neurons.

      (Inoshita et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Vps35Scer\UAS.cIa
      Vps35UAS.cIa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Inoshita
      Secondary FlyBase IDs
        References (2)