UASt regulatory sequences drive expression of a mutated form of Vps35 that carries an amino acid substitution equivalent to L774M in the orthologous human VPS35 gene, a change whose pathogenicity is uncertain (the variant has been found both in sporadic patients with Parkinson's disease, but also in unaffected individuals). (FlyBase curator comment: the mutation in the Vps35 gene is given as L800M in FBrf0229809, however analysis of the release 6.32 annotated gene model indicates the change to be L798M).
C22189198A
L779M | Vps35-PA; L798M | Vps35-PB
L800M
Analogous L774M mutation in human VPS35 implicated in Parkinson disease 17; mutation carried on in vitro construct; site of nucleotide substitution in fly gene inferred by FlyBase curator based on reported amino acid change.
Ubiquitous expression of Vps35Scer\UAS.L800M in wild type background does not result in dominant toxicity. Expression of Vps35Scer\UAS.L800M in a Vps35 mutant background largely rescues the phenotypes, but locomotor defects are still observed.
Lifespan and motor ability are unaffected when Vps35Scer\UAS.L800M is expressed under the control of Scer\GAL4da.PU at 25[o]C. Lifespan is moderately shortened compared with controls at 29[o]C.
No overt eye phenotypes are observed when Vps35Scer\UAS.L800M is expressed under the control of Scer\GAL4GMR.PU.
Flies expressing Vps35Scer\UAS.L800M under the control of Scer\GAL4da.PU in a Vps35MH20/Vps35E42 mutant background show similar climbing ability to controls.
Vps35UAS.L800M/Scer\GAL4da.PU is a suppressor of abnormal oxidative stress response phenotype of park25
Vps35UAS.L800M/Scer\GAL4da.PU is a suppressor of abnormal locomotor behavior | adult stage phenotype of park25
Expression of Vps35Scer\UAS.L800M under the control of Scer\GAL4da.PU suppresses the climbing defects and increased sensitivity to paraquat seen in park25 mutants.
Vps35UAS.L800M/Scer\GAL4da.PU partially rescues Vps35MH20/Vps35E42
Expression of Vps35Scer\UAS.L800M under the control of Scer\GAL4da.PU at 25[o] fails to rescue the phenotypes seen in Vps35MH20/Vps35E42 mutant flies. Expressing Vps35Scer\UAS.L800M at 29[o]C prevents the appearance of melanotic masses and fully rescues the developmental lethality, with progeny appearing in expected proportions.
Expression of Vps35Scer\UAS.L800M under the control of Scer\GAL4da.PU at 29[o] rescues the NMJ phenotypes seen in Vps35MH20/Vps35E42 mutant larvae.
Expression of Vps35Scer\UAS.L800M under the control of Scer\GAL4da.PU partially rescues the larval locomotion defects seen in Vps35MH20/Vps35E42 mutants.