FlyBase Allele Report: Dmel\EndoA[Δ4]

General Information

Symbol

Dmel\EndoA^Δ4

Species

D. melanogaster

Name

FlyBase ID

FBal0135727

Feature type

allele

Associated gene

Dmel\EndoA

Associated Insertion(s)

Carried in Construct

Also Known As

endo^Δ4

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Verstreken et al., 2002)

Progenitor genotype

EndoA^EP3502

(Verstreken et al., 2002)

Cytology

Description

Excision of the P{EP} element from endoA^EP3502 has deleted between the insertion site (499 bp upstream of the endoA start codon) and 37 bp before the stop codon.

(Verstreken et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | adult stage (with EndoA²⁶), with EndoA^UAS.cBa, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

abnormal neuroanatomy

(Dickman et al., 2006)

abnormal neuroanatomy (with EndoA^EP927)

(Dickman et al., 2006)

abnormal neuroanatomy (with EndoA^EP927), with Scer\GAL4^rn-GAL4-14

(Dickman et al., 2006)

abnormal neurophysiology

(Dickman et al., 2005)

abnormal neurophysiology | adult stage | conditional (with EndoA²⁶), with EndoA^S75A.CyPet

(Soukup et al., 2016)

abnormal neurophysiology | adult stage | conditional (with EndoA²⁶), with EndoA^S75D.CyPet

(Soukup et al., 2016)

abnormal neurophysiology | adult stage | progressive | somatic clone | conditional

(Soukup et al., 2016)

decreased cell number | adult stage (with EndoA²⁶), with EndoA^D265A.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

decreased cell number | adult stage (with EndoA²⁶), with EndoA^D265R.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

decreased cell number | adult stage | conditional (with EndoA²⁶), with EndoA^S75A.CyPet

(Soukup et al., 2016)

decreased cell number | adult stage | conditional (with EndoA²⁶), with EndoA^S75D.CyPet

(Soukup et al., 2016)

decreased cell number | adult stage | somatic clone | conditional

(Soukup et al., 2016)

lethal | recessive

(Verstreken et al., 2002)

lethal - all die before end of larval stage (with EndoA²⁶)

(Bademosi et al., 2023)

lethal - all die before end of second instar larval stage

(Jung et al., 2010)

short lived (with EndoA²⁶), with EndoA^D265A.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

short lived (with EndoA²⁶), with EndoA^D265R.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

some die during second instar larval stage

(Jung et al., 2010)

uncoordinated (with EndoA²⁶), with EndoA^D265A.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

uncoordinated (with EndoA²⁶), with EndoA^D265R.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

viable, with EndoA^Tag:CALI(4C)

(Soukup et al., 2016)

visible | adult stage (with EndoA²⁶), with EndoA^D265A.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

visible | adult stage (with EndoA²⁶), with EndoA^D265R.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

Phenotype Manifest In

autolysosome | increased number | larval stage | nutrition conditional (with EndoA²⁶), with EndoA^S75D.CyPet

(Hernandez-Diaz et al., 2022)

autophagosome | increased number | larval stage | nutrition conditional (with EndoA²⁶), with EndoA^S75D.CyPet

(Hernandez-Diaz et al., 2022)

autophagosome | third instar larval stage | conditional, with EndoA^Tag:CALI(4C)

(Soukup et al., 2016)

autophagosome | third instar larval stage | nutrition conditional (with EndoA²⁶), with EndoA^S75A.CyPet

(Soukup et al., 2016)

autophagosome | third instar larval stage | nutrition conditional (with EndoA²⁶), with EndoA^S75D.CyPet

(Soukup et al., 2016)

dopaminergic PPL1 neuron | adult stage | decreased number (with EndoA²⁶), with EndoA^D265A.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

dopaminergic PPL1 neuron | adult stage | decreased number (with EndoA²⁶), with EndoA^D265R.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

dopaminergic PPL1 neuron | adult stage | progressive (with EndoA²⁶), with EndoA^S75A.CyPet

(Soukup et al., 2016)

dopaminergic PPL1 neuron | adult stage | progressive (with EndoA²⁶), with EndoA^S75D.CyPet

(Soukup et al., 2016)

dopaminergic PPM2 neuron | adult stage | decreased number (with EndoA²⁶), with EndoA^D265A.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

dopaminergic PPM2 neuron | adult stage | decreased number (with EndoA²⁶), with EndoA^D265R.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

dopaminergic PPM3 neuron | adult stage (with EndoA²⁶), with EndoA^UAS.cBa, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

dopaminergic PPM3 neuron | adult stage | decreased number (with EndoA²⁶), with EndoA^D265R.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

dopaminergic PPM3 neuron | adult stage | progressive (with EndoA²⁶), with EndoA^S75A.CyPet

(Soukup et al., 2016)

dopaminergic PPM3 neuron | adult stage | progressive (with EndoA²⁶), with EndoA^S75D.CyPet

(Soukup et al., 2016)

embryonic/larval neuromuscular junction | larval stage | nutrition conditional (with EndoA²⁶), with EndoA^S75D.CyPet

(Hernandez-Diaz et al., 2022)

eye, with EndoA^A66W.UAS, Scer\GAL4^elav-C155

(Jung et al., 2010)

lamina | adult stage | conditional (with EndoA²⁶), with EndoA^S75A.CyPet

(Soukup et al., 2016)

lamina | adult stage | conditional (with EndoA²⁶), with EndoA^S75D.CyPet

(Soukup et al., 2016)

lamina | adult stage | somatic clone | conditional

(Soukup et al., 2016)

NMJ bouton | increased number

(Dickman et al., 2006)

NMJ bouton | increased number (with EndoA^EP927)

(Dickman et al., 2006)

NMJ bouton | increased number (with EndoA^EP927), with Scer\GAL4^rn-GAL4-14

(Dickman et al., 2006)

NMJ bouton | larval stage | nutrition conditional (with EndoA²⁶), with EndoA^S75D.CyPet

(Hernandez-Diaz et al., 2022)

ommatidium | adult stage | conditional (with EndoA²⁶), with EndoA^S75A.CyPet

(Soukup et al., 2016)

ommatidium | adult stage | conditional (with EndoA²⁶), with EndoA^S75D.CyPet

(Soukup et al., 2016)

ommatidium | adult stage | somatic clone | conditional

(Soukup et al., 2016)

photoreceptor neuron | adult stage | conditional (with EndoA²⁶), with EndoA^S75A.CyPet

(Soukup et al., 2016)

photoreceptor neuron | adult stage | conditional (with EndoA²⁶), with EndoA^S75D.CyPet

(Soukup et al., 2016)

photoreceptor neuron | adult stage | somatic clone | conditional

(Soukup et al., 2016)

presynaptic active zone

(Dickman et al., 2006)

presynaptic active zone (with EndoA^EP927)

(Dickman et al., 2006)

presynaptic active zone (with EndoA^EP927), with Scer\GAL4^rn-GAL4-14

(Dickman et al., 2006)

rhabdomere | adult stage | conditional (with EndoA²⁶), with EndoA^S75A.CyPet

(Soukup et al., 2016)

rhabdomere | adult stage | conditional (with EndoA²⁶), with EndoA^S75D.CyPet

(Soukup et al., 2016)

rhabdomere | adult stage | somatic clone | conditional

(Soukup et al., 2016)

synapse (with EndoA^EP927)

(Verstreken et al., 2002)

wing (with EndoA²⁶), with EndoA^D265A.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

wing (with EndoA²⁶), with EndoA^D265R.UAS, Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

Detailed Description

Statement

Reference

EndoA^Δ4/EndoA²⁶, EndoA^S75D.CyPet larvae show increased autophagosomes (Atg8-mCherry) at NMJ boutons, under both fed and starved conditions.

(Hernandez-Diaz et al., 2022)

EndoA^Δ4 heterozygotes containing a copy of EndoA^T:Zzzz\4C are viable. In these individuals, light-induced inactivation of EndoA^T:Zzzz\4C at the presynaptic region of third instar larval neuromuscular junctions results in significant decreases in the local level of endocytosis and in the local formation of autophagosomes (detected as fluorescent Atg8 puncta in confocal microscopy images) in response to electric-stimulation, as compared to 'non-inactivated' and wild-type controls.

In EndoA^Δ4/EndoA²⁶ transheterozygotes containing a copy of EndoA^{T:Avic\GFP-CyPet}, the presynaptic region of third instar larval neuromuscular junctions does not present abnormal density of autophagosome (detected as fluorescent puncta of Atg8 or Lamp in confocal microscopy images) under normal diet, nor defects in starvation-induced formation of autophagosomes, as compared to controls. In EndoA^Δ4/EndoA²⁶ transheterozygotes containing a copy of EndoA^{S75A.T:Avic\GFP-CyPet}, the presynaptic region of third instar larval neuromuscular junctions fails to present the expected starvation-induced formation of autophagosomes (detected as fluorescent puncta of Atg8 or Lamp in confocal microscopy images), but presents no defects in autophagosome density under normal diet, as compared to controls; the corresponding third instar larval effector neuron axons do not present defects in autophagosome density, as compared to controls. In EndoA^Δ4/EndoA²⁶ transheterozygotes containing a copy of EndoA^{S75D.T:Avic\GFP-CyPet}, the presynaptic region of third instar larval neuromuscular junctions presents an abnormally high density of autophagosomes (detected as fluorescent puncta of Atg8 or Lamp in confocal microscopy images) under normal diet, but does not present defects in the starvation-induced autophagosome formation, as compared to controls.

EndoA^Δ4/EndoA²⁶ transheterozygous adults containing a copy of either EndoA^{S75A.T:Avic\GFP-CyPet} or EndoA^{S75D.T:Avic\GFP-CyPet} exhibit significant decreases in the number of dopaminergic PPM3 and PPL1 neurons, but not dopaminergic PPM1 and PPM2 neurons, at day 30, but not day 1, after eclosion, as compared to controls; in adults reared under constant light, but not under constant dark, the eye exhibits a significant decrease in the rhabdomere number per ommatidium and in the photoreceptor terminal number per lamina cartridge (at day 7 of light exposure), as compared to controls; the eye of individuals reared under either constant light or constant dark also exhibits a significant decrease in depolarization amplitude in response to light pulses (at day 3 of light exposure), as compared to controls.

EndoA^Δ4-homozygous mutant eyes within EndoA^Δ4-heterozygous adults previously reared under constant light, but not within adults previously reared under constant dark, exhibit a progressive decrease in depolarization amplitude in response to light pulses (from day 2 to day 7 of light exposure) and exhibit significant decreases in the rhabdomere number per ommatidium and in the photoreceptor terminal number per lamina cartridge at day 7 of light exposure, as compared to controls.

(Soukup et al., 2016)

The neuromuscular junctions (NMJs) of third instar larvae homozygous for endoA^Δ4 exhibit numerous satellite boutons, i.e., small supernumerary boutons protruding from the primary terminal axis. This phenotype is fully penetrant at all neuromuscular junctions. Control larvae exhibit very few satellite boutons (2.48 per NMJ), whereas endoA^Δ4 exhibit a 10-fold increase (30.8 per NMJ). No abnormalities are apparent at the end of embryogenesis.

endoA^Δ4 mutant synapses exhibit approximately 65% more active zones than control synapses.

endoA^Δ4/endoA^EP927 larvae exhibit supernumerary boutons at neuromuscular junctions (a 10-fold increase, 30.5 compared to 2.48 satellite boutons/synapse). The boutons remain confined to the region of the muscle that normally receives the innervation, but this region is now crowded by the overgrowth of small boutons. No abnormalities are apparent at the end of embryogenesis.

(Dickman et al., 2006)

The amplitude of miniature excitatory junction potentials (mEJPs) is increased by 50% in endoA^Δ4 larvae compared to controls. Stimulation of endoA^Δ4 at 10 Hz for 10 minutes results in the release of a lower total number of quanta compared to wild-type larvae (~80,000 vs ~350,000). Monitoring of the recovery of the vesicle pool following stimulation shows that endoA^Δ4 vesicle pools are able to recover, but at a much slower rate than wild-type pools. The mEJP amplitude distribution is lower in endoA^Δ4 larvae following a tetanus challenge than in wild-type larvae.

endoA^Δ4 synaptic vesicles are able to load lipophilic dyes, but at a greatly reduced rate compared to wild-type vesicles, indicative of slowed endocytosis. Endocytotic rates increase from 1 to 5 Hz in endoA^Δ4 synapses, but are saturated by ~7 Hz stimulation, with synapses consistently recycling ~80 vesicles/s from 7 to 20 Hz. In contrast, endocytotic rates increase from 1 to 20 Hz stimulation in wild-type synapses with recycling up to at least 360 vesicles/s.

(Dickman et al., 2005)

Neuromuscular junction synapse in endoA^EP927/endoA^Δ4 animals are enlarged. The surface area of type I boutons are 160um² for endoA^EP927/endoA^Δ4 animals, compared to 65um² in controls.

(Verstreken et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

EndoA^Δ4 has lethal | second instar larval stage phenotype, suppressible by Scer\GAL4^elav-C155/EndoA::Hsap\ARFIP2^UAS.Tag:HA

(Jung et al., 2010)

EndoA^Δ4 has abnormal neurophysiology | third instar larval stage phenotype, suppressible by Scer\GAL4^elav-C155/EndoA::Hsap\ARFIP2^UAS.Tag:HA

(Jung et al., 2010)

NOT suppressed by

Statement

Reference

EndoA^Δ4 has lethal | second instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/CG8176::EndoA^UAS.cJa

(Jung et al., 2010)

EndoA^Δ4 has lethal | second instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Amph::EndoA^UAS.Tag:HA

(Jung et al., 2010)

EndoA^Δ4 has lethal | second instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/EndoA::Hsap\TRIP10^UAS.cJa

(Jung et al., 2010)

Enhancer of

Statement

Reference

EndoA^Δ4/EndoA[+] is an enhancer of abnormal neuroanatomy | third instar larval stage | semidominant phenotype of cup^Δ212

(Menon et al., 2015)

NOT Enhancer of

Statement

Reference

EndoA^Δ4 is a non-enhancer of visible phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

Suppressor of

Statement

Reference

endoA[+]/EndoA^Δ4 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of Lrrk^e03680

(Matta et al., 2012)

NOT Suppressor of

Statement

Reference

EndoA^Δ4/EndoA[+] is a non-suppressor of abnormal neurophysiology | dominant | third instar larval stage phenotype of Vps35^MH20

(Inoshita et al., 2017)

EndoA^Δ4 is a non-suppressor of visible phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

EndoA²⁶/EndoA^Δ4, EndoA^S75A.CyPet has autophagosome | third instar larval stage | nutrition conditional phenotype, non-suppressible by Scer\GAL4^da.G32/Hsap\LRRK2^{G2019S.UAS.Tag:V5}

(Soukup et al., 2016)

Enhancer of

Statement

Reference

EndoA^Δ4/EndoA[+] is an enhancer of NMJ bouton | third instar larval stage | increased number phenotype of cup^Δ212

(Menon et al., 2015)

NOT Enhancer of

Statement

Reference

EndoA^Δ4 is a non-enhancer of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

NOT Suppressor of

Statement

Reference

EndoA²⁶, EndoA^Δ4, EndoA^S75D.CyPet is a non-suppressor of autophagosome | larval stage | decreased number | nutrition conditional phenotype of EndoB⁴

(Hernandez-Diaz et al., 2022)

EndoA²⁶, EndoA^Δ4, EndoA^S75D.CyPet is a non-suppressor of embryonic/larval neuromuscular junction | larval stage | nutrition conditional phenotype of EndoB⁴

(Hernandez-Diaz et al., 2022)

EndoA²⁶, EndoA^Δ4, EndoA^S75D.CyPet is a non-suppressor of NMJ bouton | larval stage | nutrition conditional phenotype of EndoB⁴

(Hernandez-Diaz et al., 2022)

EndoA²⁶, EndoA^Δ4, EndoA^S75D.CyPet is a non-suppressor of autolysosome | larval stage | decreased number | nutrition conditional phenotype of EndoB⁴

(Hernandez-Diaz et al., 2022)

EndoA^Δ4 is a non-suppressor of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for EndoA^Δ4 does not significantly affect the decreased excitatory junction potential amplitude under high stimulation conditions observed at the third instar larval neuromuscular junction of Vps35^MH20 heterozygotes.

(Inoshita et al., 2017)

cup^Δ212/+;EndoA^Δ4/+ transheterozygotes show significant increases in satellite boutons at the NMJ of third instar larvae compared to either single heterozygote.

(Menon et al., 2015)

endoA^Δ4/+ suppresses the reduced excitatory junctional potential amplitude which is seen at the neuromuscular junction of Lrrk^e03680 third instar larvae during 10Z stimulation in 2mM calcium.

(Matta et al., 2012)

Expression of Amph::endoA^{Scer\UAS.T:Ivir\HA} or CG8176::endoA^Scer\UAS.cJa using Scer\GAL4^elav-C155 fails to rescue endoA^Δ4 larval lethality to adulthood.

(Jung et al., 2010)

Xenogenetic Interactions

Statement

Reference

The defective starvation-induced formation of autophagosomes observed at the presynaptic region of the third instar larval neuromuscular junction of EndoA^Δ4/EndoA²⁶ transheterozygotes that also possess one copy of EndoA^{S75A.T:Avic\GFP-CyPet} is not rescued by the expression of Hsap\LRRK2^{G2019S.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^Toll-6-D42.

(Soukup et al., 2016)

Expression of endoA::Hsap\ARFIP2^{Scer\UAS.T:Ivir\HA} using Scer\GAL4^elav-C155 rescues endoA^Δ4 larval lethality to adulthood, although the adult rescuants cannot fly and have reduced locomotor activity, and they have a median survival time of only 1.5 days.

Scer\GAL4^elav-C155-driven expression of endoA::Hsap\ARFIP2^{Scer\UAS.T:Ivir\HA} rescues the severe tetanus-induced depression in excitatory junctional potentials (EJPs), the slow post-tetanic recovery of EJP amplitude, and the low frequency of spontaneous miniature EJPs seen in endoA^Δ4 mutants.

Expression of endoA::Hsap\TRIP10^Scer\UAS.cJa using Scer\GAL4^elav-C155 fails to rescue endoA^Δ4 larval lethality to adulthood.

(Jung et al., 2010)

Complementation and Rescue Data

Rescued by

EndoA²⁶/EndoA^Δ4 is rescued by EndoA^D265A.UAS/Scer\GAL4^GMR57C10

(Bademosi et al., 2023)

EndoA²⁶/EndoA^Δ4 is rescued by Scer\GAL4^GMR57C10/EndoA^D265R.UAS

(Bademosi et al., 2023)

EndoA²⁶/EndoA^Δ4 is rescued by Scer\GAL4^GMR57C10/EndoA^UAS.cBa

(Bademosi et al., 2023)

EndoA^Δ4 is rescued by Scer\GAL4^elav-C155/EndoA^UAS.wt

(Jung et al., 2010)

EndoA^Δ4 is rescued by EndoA^UAS.Tag:HA/Scer\GAL4^elav-C155

(Jung et al., 2010)

EndoA^Δ4 is rescued by Scer\GAL4^elav-C155/EndoA^A66D.UAS

(Jung et al., 2010)

EndoA^Δ4 is rescued by Scer\GAL4^elav-C155/EndoA^SS.UAS

(Jung et al., 2010)

EndoA^Δ4 is rescued by Scer\GAL4^elav-C155/EndoA^SSQ.UAS

(Jung et al., 2010)

EndoA^Δ4 is rescued by Scer\GAL4^elav-C155/EndoA^D151P.UAS

(Jung et al., 2010)

Partially rescued by

EndoA^Δ4 is partially rescued by Scer\GAL4^elav-C155/EndoA^A66W.UAS

(Jung et al., 2010)

Not rescued by

EndoA^Δ4 is not rescued by Scer\GAL4^elav-C155/EndoA^ΔLQPNP.UAS

(Jung et al., 2010)

EndoA^Δ4 is not rescued by Scer\GAL4^elav-C155/EndoA^EEE.UAS

(Jung et al., 2010)

EndoA^Δ4 is not rescued by Scer\GAL4^elav-C155/EndoA^I10E.UAS

(Jung et al., 2010)

EndoA^Δ4 is not rescued by Scer\GAL4^elav-C155/EndoA^{P143A.ΔLEN.UAS}

(Jung et al., 2010)

EndoA^Δ4 is not rescued by Scer\GAL4^elav-C155/EndoA^ΔBAR.UAS

(Jung et al., 2010)

Comments

Expression of endoA^Scer\UAS.wt using Scer\GAL4^elav-C155 rescues endoA^Δ4 larval lethality - animals survive to adulthood, although the adult rescuants cannot fly and have a median survival time of only 6.3 days.

Expression of endoA^{Scer\UAS.T:Ivir\HA} using Scer\GAL4^elav-C155 rescues endoA^Δ4 larval lethality - animals survive to adulthood, although the median survival time of adult rescuants is only 5.7 days.

Expression of endoA^{A66D.Scer\UAS} using Scer\GAL4^elav-C155 rescues endoA^Δ4 larval lethality - animals survive to adulthood, although the adult rescuants cannot fly and have a median survival time of only 6.5 days.

Depending on the insertion, Scer\GAL4^elav-C155-driven expression of endoA^{A66W.Scer\UAS} causes embryonic or pupal lethality, or flies die a few days after eclosion exhibiting uninflated wings and a small-eye phenotype. Additional eye phenotypes in flies surviving to adulthood include: unusually narrow and pointed aspect of the lower eye; roughening of the eye caused by ommatidia that are uneven in size and missing/supernumerary bristles; abnormal pitting in the ommatidia.

Expression of endoA^SS.Scer\UAS using Scer\GAL4^elav-C155 rescues endoA^Δ4 larval lethality - animals survive to adulthood, although the adult rescuants cannot fly and have reduced locomotor activity, and they have a median survival time of only 4.5 days.

Expression of endoA^SSQ.Scer\UAS using Scer\GAL4^elav-C155 rescues endoA^Δ4 larval lethality - animals survive to adulthood, although the adult rescuants cannot fly and have reduced locomotor activity, and they have a median survival time of only 2.3 days.

Expression of endoA^{ΔLQPNP.Scer\UAS} using Scer\GAL4^elav-C155 fails to rescue endoA^Δ4 larval lethality to adulthood.

Expression of endoA^EEE.Scer\UAS using Scer\GAL4^elav-C155 fails to rescue endoA^Δ4 larval lethality to adulthood.

Expression of endoA^{I10E.Scer\UAS} using Scer\GAL4^elav-C155 fails to rescue endoA^Δ4 larval lethality to adulthood.

Expression of endoA^{D151P.Scer\UAS} using Scer\GAL4^elav-C155 rescues endoA^Δ4 larval lethality - animals survive to adulthood, although the adult rescuants cannot fly and have a median survival time of only 6 days.

Expression of endoA^{P143A.ΔLEN.Scer\UAS} using Scer\GAL4^elav-C155 fails to rescue endoA^Δ4 larval lethality to adulthood.

Expression of endoA^{ΔBAR.Scer\UAS} using Scer\GAL4^elav-C155 fails to rescue endoA^Δ4 larval lethality to adulthood.

Scer\GAL4^elav-C155-driven expression of endoA^Scer\UAS.wt, endoA^{Scer\UAS.T:Ivir\HA}, endoA^SS.Scer\UAS, endoA^SSQ.Scer\UAS, endoA^{A66D.Scer\UAS}, endoA^{D151P.Scer\UAS} or endoA^{A66W.Scer\UAS} rescues the severe tetanus-induced depression in excitatory junctional potentials (EJPs), the slow post-tetanic recovery of EJP amplitude, and the low frequency of spontaneous miniature EJPs seen in endoA^Δ4 mutants.

(Jung et al., 2010)

Overexpression of endoA^EP927, under the control of Scer\GAL4^elav-C155 in a endoA^EP927/endoA^Δ4 background restores wild-type structure to the synapse, with the same average number of boutons per synapse as in wild-type.

Overexpression of endoA^EP927, under the control of Scer\GAL4^rn-GAL4-14 in a endoA^EP927/endoA^Δ4 background fails to restore wild-type structure to the synapse.

(Dickman et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24882

w^*; EndoA^Δ4/TM3, P{GAL4-Kr.C}DC2, P{UAS-GFP.S65T}DC10, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

EndoA^Δ4

(Inoshita et al., 2017)

endo^Δ4

(Dickman, 2006, Dickman et al., 2006, Verstreken et al., 2002)

endoA^delta4

(Hernandez-Diaz et al., 2022)

endoA^Δ4

(Bademosi et al., 2023, Soukup et al., 2016, Chance and Bashaw, 2015, Menon et al., 2015, Matta et al., 2012, Jung et al., 2010, Buff et al., 2007, Dickman et al., 2005)

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