UASt regulatory sequences drive expression of mutant Hsap\VPS35 (D620N).
Ubiquitous expression of a transgene containing a variant of human VPS35 linked to dominantly inherited Parkinson's disease (Hsap\VPS35D620N.Scer\UAS) in a wild type background does not result in dominant toxicity. Expression of Hsap\VPS35D620N.Scer\UAS in a Vps35 mutant background only partially rescues the locomotor phenotypes.
Lifespan and motor ability are unaffected when Hsap\VPS35D620N.Scer\UAS is expressed under the control of Scer\GAL4da.PU at 25[o]C. Lifespan is moderately shortened compared with controls at 29[o]C.
No overt eye phenotypes are observed when Hsap\VPS35D620N.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.
Scer\GAL4da.PU/Hsap\VPS35D620N.UAS is a suppressor | partially of abnormal locomotor behavior | larval stage phenotype of Vps35MH20/Vps35E42
Scer\GAL4da.PU/Hsap\VPS35D620N.UAS is a suppressor of abnormal oxidative stress response phenotype of park25
Scer\GAL4da.PU/Hsap\VPS35D620N.UAS is a suppressor of abnormal locomotor behavior | adult stage phenotype of park25
Scer\GAL4da.PU/Hsap\VPS35D620N.UAS is a non-suppressor of lethal phenotype of Vps35MH20/Vps35E42
Expression of Hsap\VPS35D620N.Scer\UAS under the control of Scer\GAL4da.PU at 25[o] fails to suppress the phenotypes seen in Vps35MH20/Vps35E42 mutant flies. Expressing Hsap\VPS35D620N.Scer\UAS at 29[o]C rescues mutant viability to the late pupal stage, but adults fail to eclose.
Expression of Hsap\VPS35D620N.Scer\UAS under the control of Scer\GAL4da.PU at 29[o] suppresses the NMJ phenotypes seen in Vps35MH20/Vps35E42 mutant larvae.
Expression of Hsap\VPS35D620N.Scer\UAS under the control of Scer\GAL4da.PU partially suppresses the larval locomotion defects seen in Vps35MH20/Vps35E42 mutants.
Expression of Hsap\VPS35D620N.Scer\UAS under the control of Scer\GAL4da.PU suppresses the climbing defects and increased sensitivity to paraquat seen in park25 mutants.