FlyBase Allele Report: Dmel\Rab5[2]

General Information

Symbol

Dmel\Rab5²

Species

D. melanogaster

Name

FlyBase ID

FBal0182047

Feature type

allele

Associated gene

Dmel\Rab5

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Lu and Bilder, 2005)

Mutagen

P-element activity

(Wucherpfennig et al., 2003)

Progenitor genotype

Rab5^k08232

(Wucherpfennig et al., 2003)

Caused by aberration

Df(2L)Rab5²

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of carcinoma

CEA

(Lu and Bilder, 2005)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 8

modeled by Vap33^Δ20

(Mao et al., 2019)

ameliorates autosomal dominant adult-onset proximal spinal muscular atrophy

modeled by Vap33^Δ20

(Mao et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal dorsal multidendritic neuron | P-stage | somatic clone

(Zhang et al., 2014)

adult antennal lobe projection neuron DL1 adPN | somatic clone

(Sakuma et al., 2014)

apical plasma membrane | somatic clone

(Schottenfeld-Roames et al., 2014)

axon | somatic clone | third instar larval stage

(Satoh et al., 2008)

dendrite | somatic clone | third instar larval stage

(Satoh et al., 2008)

dendritic tree | P-stage | somatic clone

(Zhang et al., 2014)

egg | germline clone

(Compagnon et al., 2009)

embryonic/larval fat body | larval stage | somatic clone

(Bourouis et al., 2019)

embryonic/larval fat body | somatic clone | third instar larval stage

(Hegedűs et al., 2016)

endocytic vesicle | germline clone

(Compagnon et al., 2009)

endosome & embryonic nervous system

(Wucherpfennig et al., 2003)

endosome | P-stage | somatic clone

(Zhang et al., 2014)

eye-antennal disc | somatic clone

(Lu and Bilder, 2005)

eye disc | somatic clone

(Menut et al., 2007)

eye photoreceptor cell | somatic clone

(Houalla et al., 2010)

follicle cell | somatic clone

(Lu and Bilder, 2005)

larval dorsal multidendritic neuron ddaC | P-stage | somatic clone

(Zhang et al., 2014)

terminal tracheal cell | somatic clone

(Schottenfeld-Roames et al., 2014)

tracheal lumen | somatic clone

(Schottenfeld-Roames et al., 2014)

wing disc | somatic clone

(Menut et al., 2007)

yolk granule | germline clone

(Compagnon et al., 2009, Morrison et al., 2008)

Detailed Description

Statement

Reference

Rab5² larval fat body clone cells are slightly but significantly larger than control cells.

(Bourouis et al., 2019)

Fat body cells in Rab5² mutant somatic clones in starved third instar larvae are larger than the surrounding control cells but show similar number and size of autophagic structures and acidic autolysosomes (detected by LysoTracker staining).

(Hegedűs et al., 2016)

Rab5² heterozygotes do not exhibit a discernible phenotype in the adult brain.

(Rowshanravan et al., 2014)

Homozygous single-cell DL1 PN clones show defects in both the axons and dendrites. The axon fails to exit the antennal lobe in 47.6% of cases. The dendrites have an additional branch appearing from the proximal side of the dendrite shaft (85.7% penetrance), and sometimes target two glomeruli instead of a single glomerulus.

(Sakuma et al., 2014)

Rab5² mutant cells display seamless tracheal tube cysts and disorganized tube termini. They also show reduced terminal branching, failure in gas filling, increased tube diameter, and extreme disorganization of the apical membrane.

(Schottenfeld-Roames et al., 2014)

Mutant egg chambers in females containing homozygous germline clones show normal accumulation of intracellular lipid droplets.

(Parra-Peralbo and Culi, 2011)

The cortical F-actin layer at the posterior pole of stage 10b oocytes is disrupted in females carrying homozygous germline clones.

(Tanaka et al., 2011)

Homozygous mutant Rab5² eye disc clones do not undergo R-cell differentiation, leading to the overgrowth of the eye imaginal discs.

(Houalla et al., 2010)

Eggs derived from Rab5² germ-line clones are flaccid, and they collapse. In Rab5² germ line clones, no condensed yolk granules are observed in the oocyte cytoplasm. Clathrin coated pits are still present at the oocyte plasma membrane. Below the plasma membrane, the density of early endocytic vesicles is increased compared to controls.

(Compagnon et al., 2009)

Oocytes derived from homozygous germline clones lack yolk granules.

(Morrison et al., 2008)

Homozygous Rab5² mutant neurons extend axons and major dendritic branches, however, they display greatly simplified dendritic arbors and decreased number of terminal branches compared with that found for wild-type neurons.

(Satoh et al., 2008)

Mutant eye discs (generated using the eyFLP-cell lethal system) show disorganised cellular architecture and reduced differentiation compared to wild type.

Larvae containing mutant eye discs generated using the eyFLP-cell lethal system are increased in size compared to control larvae when examined as pupariation commences. The animals do not develop into adults.

Wing discs contain regions of neoplastic overgrowth in animals in which clones have been induced in the wing, haltere and leg discs (using the UbxFLP-cell lethal system). Eye discs are normal in these animals.

(Menut et al., 2007)

Eye imaginal discs that consist predominantly of Rab5² mutant clones (produced using the "eyFLP/Minute" system) and Rab5² mutant follicle cells show multilayered, overproliferative phenotypes very similar to Syx¹ mutant tissues.

(Lu and Bilder, 2005)

Zygotic loss of Rab5² results in the disruption of endosomes in the embryonic nervous system. In control embryos, the Avic\GFP^{Scer\UAS.T:Hsap\MYC,T:Mmus\2xFYVE}-positive endosomes appear as punctate structures within the cell bodies of the central nervous system, peripheral nervous system and neuromuscular junctions. Avic\GFP^{Scer\UAS.T:Hsap\MYC,T:Mmus\2xFYVE}-positive endosomes are disrupted in the nervous system and neuromuscular junctions of Rab5² mutant embryos.

(Wucherpfennig et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Df(1)rush⁴, Rab5², sta^+t4.4 has partially lethal - majority live phenotype, suppressible | partially by rush^UAS.GFP/Scer\GAL4^arm.PS

(Gailite et al., 2012)

NOT suppressed by

Statement

Reference

Rab5² has abnormal cell size | somatic clone | third instar larval stage phenotype, non-suppressible by Mon1^mut4/Mon1^mut4

(Hegedűs et al., 2016)

Suppressor of

Statement

Reference

Rab5² is a suppressor | partially of decreased cell number | adult stage | progressive phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Rab5²/Rab5[+] is a suppressor of abnormal neuroanatomy phenotype of vap²

(Rowshanravan et al., 2014)

Other

Statement

Reference

Df(1)rush⁴, Rab5², sta^+t4.4 has partially lethal - majority live phenotype

(Gailite et al., 2012)

Rab5²/Rab5[+], Scer\GAL4^en-e16E, Vps45^RNAi.UAS.WIZ has visible phenotype

(Morrison et al., 2008)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Rab5² has embryonic/larval fat body | somatic clone | third instar larval stage phenotype, non-suppressible by Mon1^mut4/Mon1^mut4

(Hegedűs et al., 2016)

Enhancer of

Statement

Reference

Rab5²/Rab5[+] is an enhancer of eye phenotype of RN-tre^UAS.cHa, Scer\GAL4^GMR.long

(Houalla et al., 2010)

Suppressor of

Statement

Reference

Rab5² is a suppressor | partially of outer photoreceptor cell | adult stage | decreased number phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Rab5²/Rab5[+] is a suppressor of adult brain phenotype of vap²

(Rowshanravan et al., 2014)

Rab5² is a suppressor of wing vein L5 phenotype of Df(3L)ru-22/rho^ve-1

(Tjota et al., 2011)

Rab5² is a suppressor of wing vein L5 phenotype of rho^ve-1

(Tjota et al., 2011)

NOT Suppressor of

Statement

Reference

Rab5²/Rab5² is a non-suppressor of embryonic/larval fat body | somatic clone | third instar larval stage phenotype of Mon1^mut4

(Hegedűs et al., 2016)

Rab5²/Rab5² is a non-suppressor of autolysosome | somatic clone | third instar larval stage phenotype of Mon1^mut4

(Hegedűs et al., 2016)

Other

Statement

Reference

Rab5²/Rab5[+], Scer\GAL4^en-e16E, Vps45^RNAi.UAS.WIZ has wing phenotype

(Morrison et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Somatic clones of fat body cell double mutant for both Rab5² and Mon1^mut4 are larger than their neighboring control cells (as are Rab5² single mutant cells) but display similar abnormal accumulation of autophagosomes and lack of acidic autolysosomes as Mon1^mut4 mutant cells in starved third instar larvae.

(Hegedűs et al., 2016)

A heterozygous Rab5² mutant background significantly suppresses the vap² neurodegenerative phenotype.

(Rowshanravan et al., 2014)

While Df(1)rush⁴, sta^+t4.4 homozygous fies and Rab5²heterozygotes are viable, their combination decreases viability at embryonic and pupal stages. This decreased survival is partially rescued by expression of rush^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^arm.PS.

(Gailite et al., 2012)

A Rab5² heterozygous background leads to full suppression of the L5 wing vein defect in rho^ve-1/Df(3L)ru-22 kst¹ mutants and 90% suppression in rho^ve-1/rho^ve-1 kst¹ flies.

(Tjota et al., 2011)

Reducing the dosage of Rab5 through a Rab5² heterozygous background significantly enhances the RN-tre^Scer\UAS.cHa-induced rough eye phenotype.

(Houalla et al., 2010)

Expression of Vps45^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^en-e16E in a Rab5²/+ background results in defects in the wing.

(Morrison et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rab5² is rescued by Rab5^UASp.YFP/Scer\GAL4^GH146

(Sakuma et al., 2014)

Rab5² is rescued by Rab5^UASp.YFP/Scer\GAL4^elav.PU

(Zhang et al., 2014)

Rab5² is rescued by Rab5^+tWa

(Wucherpfennig et al., 2003)

Comments

Expression of Rab5^{Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^GH146 rescues the defects seen in homozygous Rab5² single-cell DL1 olfactory projection neuron clones.

(Sakuma et al., 2014)

Rab5^+tWa rescues the lethality due to Rab5².

(Wucherpfennig et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

42702

P{hsFLP}1, y¹ w^*; Rab5² P{neoFRT}40A/In(2LR)Gla, wg^Gla-1 PPO1^Bc

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Rab5-null

(Satoh et al., 2008)

Rab5^KO

(Mao et al., 2019)

Rab5^d2

(Hegedűs et al., 2016)

rab5²

(Tsarouhas et al., 2019, Luo et al., 2016, Romani et al., 2016, Chance and Bashaw, 2015, Parra-Peralbo and Culi, 2011, Tanaka et al., 2011, Compagnon et al., 2009, Menut et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (37)

Report Sections

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