FlyBase Allele Report: Dmel\Rab5[UASp.YFP]

General Information

Symbol

Dmel\Rab5^UASp.YFP

Species

D. melanogaster

Name

FlyBase ID

FBal0215392

Feature type

allele

Associated gene

Dmel\Rab5

Associated Insertion(s)

Carried in Construct

P{UASp-YFP.Rab5}

Also Known As

UAS-YFP-Rab5, UAS-YFP:Rab5, UAS-Rab5-YFP, UAS-Rab5, YFP-Rab5, Rab5-WT

Key Links

Transgenic product class

wild_type

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-YFP.Rab5}

Cytology

Description

UASp regulatory sequences drive expression of wild-type Rab5 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rab5

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates motor neuron disease

modeled by scat^HMS01910

(Patel et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | larval stage, with Scer\GAL4^futsch-C380

(Patel et al., 2020)

Phenotype Manifest In

embryonic/larval plasmatocyte | larval stage, with Scer\GAL4^Hml.Δ

(Petrignani et al., 2021)

follicle cell, with Scer\GAL4^55B

(Woolworth et al., 2009)

Detailed Description

Statement

Reference

Larval macrophages expressing Rab5^UASp.YFP under the control of Scer\GAL4^Hml.Δ exhibit increased number of large intracellular vesicles.

(Petrignani et al., 2021)

The expression of Rab5^{Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^ap-md544 has no obvious effect on the adult wing morphology, as compared to control.

(O'Farrell et al., 2017)

Expression of Rab5^{Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^Appl.G1a does not impair axonal transport in the segmental nerves in third instar larvae.

(Xu et al., 2016)

Expression via Scer\GAL4^pnr-MD237 results in flies with a wild type notum.

(Thomas et al., 2009)

Epithelial defects are seen in the egg chambers of females expressing Rab5^{Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^55B; 11% show cell swelling and 5% show piling up or loose cells.

(Woolworth et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Rab5^UASp.YFP, Scer\GAL4^ap-md544 is an enhancer of visible phenotype of Pi3K59F^KD.UAS, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

NOT Enhancer of

Statement

Reference

Rab5^UASp.YFP/Scer\GAL4^pnt-14-94 is a non-enhancer of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab5^UASp.YFP/Scer\GAL4^pnt-14-94 is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab5^UASp.YFP is a non-enhancer of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab5^UASp.YFP is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Suppressor of

Statement

Reference

Rab5^UASp.YFP, Scer\GAL4^c306 is a suppressor | partially of abnormal cell polarity | oogenesis phenotype of Scer\GAL4^c306, cactin^KK100507

(Miao et al., 2022)

Rab5^UASp.YFP, Scer\GAL4^c306 is a suppressor | partially of abnormal cell migration | oogenesis phenotype of Scer\GAL4^c306, cactin^KK100507

(Miao et al., 2022)

Rab5^UASp.YFP, Scer\GAL4^futsch-C380 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Scer\GAL4^futsch-C380, scat^HMS01910

(Patel et al., 2020)

NOT Suppressor of

Statement

Reference

Rab5^UASp.YFP/Scer\GAL4^pnt-14-94 is a non-suppressor of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab5^UASp.YFP is a non-suppressor of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab5^UASp.YFP/Scer\GAL4^pnt-14-94 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab5^UASp.YFP is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab5^UASp.YFP, Scer\GAL4^Appl.G1a is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Phenotype Manifest In

Enhancer of

Statement

Reference

Rab5^UASp.YFP, Scer\GAL4^ap-md544 is an enhancer of wing phenotype of Pi3K59F^KD.UAS, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

NOT Enhancer of

Statement

Reference

Scer\GAL4^insc-Mz1407/Rab5^UASp.YFP is a non-enhancer of embryonic/larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab5^UASp.YFP/Scer\GAL4^pnt-14-94 is a non-enhancer of larval neuroblast | larval stage | increased number phenotype of Vps35^E42

(Li et al., 2018)

Rab5^UASp.YFP/Scer\GAL4^pnt-14-94 is a non-enhancer of embryonic/larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab5^UASp.YFP is a non-enhancer of larval neuroblast | larval stage | increased number phenotype of Vps35^E42

(Li et al., 2018)

Rab5^UASp.YFP, Scer\GAL4^btl.PS is a non-enhancer of embryonic/larval tracheal dorsal trunk phenotype of Scer\GAL4^btl.PS, Vhl^RNAi.UAS

(Hsouna et al., 2010)

Suppressor of

Statement

Reference

Rab5^UASp.YFP, Scer\GAL4^c306 is a suppressor | partially of border follicle cell phenotype of Scer\GAL4^c306, cactin^KK100507

(Miao et al., 2022)

Rab5^UASp.YFP, Scer\GAL4^futsch-C380 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of Scer\GAL4^futsch-C380, scat^HMS01910

(Patel et al., 2020)

Rab5^UASp.YFP, Scer\GAL4^futsch-C380 is a suppressor | partially of NMJ bouton | increased number | larval stage phenotype of Scer\GAL4^futsch-C380, scat^HMS01910

(Patel et al., 2020)

Rab5^UASp.YFP, Scer\GAL4^55B is a suppressor | partially of follicle cell phenotype of Scer\GAL4^55B, awd^RNAi.UAS

(Woolworth et al., 2009)

NOT Suppressor of

Statement

Reference

Rab5^UASp.YFP/Scer\GAL4^pnt-14-94 is a non-suppressor of embryonic/larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab5^UASp.YFP is a non-suppressor of embryonic/larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab5^UASp.YFP/Scer\GAL4^pnt-14-94 is a non-suppressor of larval neuroblast | increased number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab5^UASp.YFP is a non-suppressor of larval neuroblast | increased number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab5^UASp.YFP, Scer\GAL4^Appl.G1a is a non-suppressor of larval segmental nerve | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Rab5^UASp.YFP, Scer\GAL4^Appl.G1a is a non-suppressor of axon | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Rab5^UASp.YFP, Scer\GAL4^btl.PS is a non-suppressor of embryonic/larval tracheal dorsal trunk phenotype of Scer\GAL4^btl.PS, Vhl^RNAi.UAS

(Hsouna et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Rab5^{Scer\UAS.P\T.T:Avic\GFP-YFP} enhances the wing defects resulting from the expression of Pi3K59F^KD.Scer\UAS under the control of Scer\GAL4^ap-md544.

(O'Farrell et al., 2017)

The penetrance of the tracheal lumen defects (convoluted and dilated dorsal trunks) caused by expression of Vhl^{dsRNA.Scer\UAS} under the control of Scer\GAL4^btl.PS is not altered by co-expression of Rab5^{Scer\UAS.P\T.T:Avic\GFP-YFP}.

(Hsouna et al., 2010)

The follicle cell epithelial defects seen in females expressing awd^{dsRNA.Scer\UAS} under the control of Scer\GAL4^55B are rescued by co-expression of Rab5^{Scer\UAS.P\T.T:Avic\GFP-YFP}.

(Woolworth et al., 2009)

Xenogenetic Interactions

Statement

Reference

The presence of axonal blockages in the segmental nerves characteristic for third instar larvae expressing Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^Appl.G1a is not rescued by co-expression of Rab5^{Scer\UAS.P\T.T:Avic\GFP-YFP}.

(Xu et al., 2016)

Complementation and Rescue Data

Rescues

Rab5^UASp.YFP/Scer\GAL4^GH146 rescues Rab5²

(Sakuma et al., 2014)

Rab5^UASp.YFP/Scer\GAL4^elav.PU rescues Rab5²

(Zhang et al., 2014)

Comments

Expression of Rab5^{Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^GH146 rescues the defects seen in homozygous Rab5² single-cell DL1 olfactory projection neuron clones.

(Sakuma et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

50788

P{UASp-YFP.Rab5}8, y¹ w^*

24616

y¹ w^*; P{UASp-YFP.Rab5}02

9775

y¹ w^*; P{UASp-YFP.Rab5}Pde8^08b