FlyBase Allele Report: Dmel\Pi3K59F[KD.UAS]

General Information

Symbol

Dmel\Pi3K59F^KD.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0243865

Feature type

allele

Associated gene

Dmel\Pi3K59F

Associated Insertion(s)

Carried in Construct

P{UAS-Pi3K59F.KD}

Also Known As

UAS-Vps34^KD, UAS-vps34^DN, UAS-Vps34-KD

Key Links

Transgenic product class

missense_variant

(Juhász et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Juhász et al., 2008)

Mutagen

in vitro construct

(Juhász et al., 2008)

Progenitor genotype

Pi3K59F^UAS.cJa

(Juhász et al., 2008)

Carried in construct

P{UAS-Pi3K59F.KD}

Cytology

Description

UAS regulatory sequences drive expression of mutated form of Pi3K59F (containing the amino acid replacements D805A and N810I), which is kinase-defective.

(Juhász et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pi3K59F

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal starvation stress response | late third instar larval stage, with Scer\GAL4^Cg.PA

(Pircs et al., 2012)

visible, with Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Pi3K59F^KD.Scer\UAS under the control of Scer\GAL4^ap-md544 leads to adult wings presenting an upward curvature.

(O'Farrell et al., 2017)

Expression of Pi3K59F^KD.Scer\UAS using Scer\GAL4^Act5C.PP results in basal autophagy defects in larval fat body cell somatic clones.

Expression of Pi3K59F^KD.Scer\UAS using Scer\GAL4^Cg.PA causes a decrease in starvation-induced autophagic response in the fat body of wandering third instar larvae compared with wild-type.

(Pircs et al., 2012)

Starvation-induced expansion of the lysosomal system is blocked in fat-body somatic clones expressing Pi3K59F^KD.Scer\UAS under the control of Scer\GAL4^Act5C.PP. Autophagosome and autolysosome formation is disrupted in these animals, and the accumulation of pre-autophagosomal membranes does not occur. Fat-body cell size is normal in these animals.

Expression of Pi3K59F^KD.Scer\UAS in the garland cells under the control of Scer\GAL4^Act5C.PP show disruptions in endocytosis.

(Juhász et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pi3K59F^KD.UAS, Scer\GAL4^ap-md544 has visible phenotype, enhanceable by Rab5^UASp.YFP, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Pi3K59F^KD.UAS, Scer\GAL4^ap-md544 has visible phenotype, enhanceable by Rab7^{Q67L.UASp.YFP}, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Suppressed by

Statement

Reference

Pi3K59F^KD.UAS, Scer\GAL4^ap-md544 has visible phenotype, suppressible by Lkb1^HMS01351, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

NOT suppressed by

Statement

Reference

Myc^UAS.cZa, Pi3K59F^KD.UAS, Scer\GAL4^unspecified has increased cell growth rate | somatic clone | third instar larval stage phenotype, non-suppressible by cnc^EY08884, Scer\GAL4^unspecified

(Nagy et al., 2013)

NOT Enhancer of

Statement

Reference

Pi3K59F^KD.UAS is a non-enhancer of increased cell size | somatic clone phenotype of Rheb^EP50.084, Scer\GAL4^Act5C.PP

(Juhász et al., 2008)

Suppressor of

Statement

Reference

Pi3K59F^KD.UAS, Scer\GAL4^ptc-559.1 is a suppressor of visible phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Pi3K59F^KD.UAS, Scer\GAL4^ptc-559.1 is a suppressor of abnormal size | third instar larval stage phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Pi3K59F^KD.UAS, Scer\GAL4^ptc-559.1 is a suppressor of abnormal size phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pi3K59F^KD.UAS, Scer\GAL4^ap-md544 has wing phenotype, enhanceable by Rab5^UASp.YFP, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Pi3K59F^KD.UAS, Scer\GAL4^ap-md544 has wing phenotype, enhanceable by Rab7^{Q67L.UASp.YFP}, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Suppressed by

Statement

Reference

Pi3K59F^KD.UAS, Scer\GAL4^ap-md544 has wing phenotype, suppressible by Lkb1^HMS01351, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

NOT suppressed by

Statement

Reference

Myc^UAS.cZa, Pi3K59F^KD.UAS, Scer\GAL4^unspecified has embryonic/larval fat body | somatic clone | third instar larval stage phenotype, non-suppressible by cnc^EY08884, Scer\GAL4^unspecified

(Nagy et al., 2013)

NOT Enhancer of

Statement

Reference

Pi3K59F^KD.UAS is a non-enhancer of fat body | somatic clone phenotype of Rheb^EP50.084, Scer\GAL4^Act5C.PP

(Juhász et al., 2008)

Suppressor of

Statement

Reference

Pi3K59F^KD.UAS, Scer\GAL4^ptc-559.1 is a suppressor of wing disc | third instar larval stage phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Pi3K59F^KD.UAS, Scer\GAL4^ptc-559.1 is a suppressor of 1st posterior cell phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Pi3K59F^KD.UAS, Scer\GAL4^ptc-559.1 is a suppressor of wing basal cell 1 phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

NOT Suppressor of

Statement

Reference

Pi3K59F^KD.UAS/Scer\GAL4^Pxn.PS is a non-suppressor of embryonic/larval hemocyte phenotype of Scer\GAL4^Cg.PA, mtm^RNAi.cVa.UAS

(Velichkova et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The upward adult wing curvature resulting from the expression of Pi3K59F^KD.Scer\UAS under the control of Scer\GAL4^ap-md544 is suppressed by the co-expression of Lkb1^HMS01351, but is enhanced by the co-expression of Rab5^{Scer\UAS.P\T.T:Avic\GFP-YFP} or Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP}.

(O'Farrell et al., 2017)

The lack of F-actin rich radial protrusions seen in primary hemocytes derived from larvae expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Pxn.PS is infrequently and only mildly modified by co-expression of Pi3K59F^KD.Scer\UAS.

(Velichkova et al., 2010)

Expression of Rheb^EP50.084 under the control of Scer\GAL4^Act5C.PP increases cell size, and this increase is also observed in the presence of Pi3K59F^KD.Scer\UAS.

(Juhász et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pi3K59F^KD.Scer\UAS

Pi3K59F^KD.UAS

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

Open Close