FlyBase Allele Report: Dmel\mtm[RNAi.cVa.UAS]

General Information

Symbol

Dmel\mtm^RNAi.cVa.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0258479

Feature type

allele

Associated gene

Dmel\mtm

Associated Insertion(s)

Carried in Construct

P{UAS-mtm.RNAi.V}

Also Known As

mtm RNAi

Key Links

Transgenic product class

RNAi_reagent

(Velichkova et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Velichkova et al., 2010)

Mutagen

in vitro construct

(Velichkova et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-mtm.RNAi.V}

Cytology

Description

UAS regulatory sequences drive expression of a 395bp inverted repeats of mtm sequences in head-to-head orientation.

(Velichkova et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mtm

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of myopathy

CEA

(Ribeiro et al., 2011)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^He.PZ results in hemocytes exhibiting abnormally enlarged endolysosomal compartments.

(Pueyo et al., 2016)

Expression of mtm^{dsRNA.cVa.Scer\UAS} under the control of either Scer\GAL4^how-24B or Scer\GAL4^Mef2.PR results in a decrease in the number of both dorsal temporary internal oblique muscles (IOMs) and ventral persistant larval muscles (PLMs) in the abdomen of pharate adults. The IOMs that are present are frequently detached. Central myofibrils are misaligned or absent around a normal number of centrally-displaced nuclei (distance of nuclei from midline is increased) in the IOMs of pharate adults expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Mef2.PR. The nuclei are otherwise normal in size and morphology, mitochondrial integrity is normal and the peripheral myofibrils appear normal. Transverse (T-) tubules are disrupted.

IOMs expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Mef2.PR are properly maintained during early pupal stages, when other larval muscles undergo developmentally regulated cell death (2 days after puparium formation, APF) and subsequently undergo normal myofiber thinning (at 3 days APF) and rethickening (at 4 days APF), but IOM muscle detachment is seen during remodeling in late pupal stages (4 days APF).

Visceral muscles that normally migrate to ensheath the testis are present but disrupted in adults expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^how-24B.

(Ribeiro et al., 2011)

Primary hemocytes dissected from wild-type larvae undergo remodelling from a round to a spread cell shape, having F-actin rich radial protrusions. However, primary hemocytes derived larvae expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Pxn.PS lack these protrusions.

Third instar larvae expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Pxn.PS have a normal number of bled hemocytes per larva and have a normal number of sessile hemocytes per segment, although the sessile hemocytes have an abnormally clumped distribution compared to controls.

Third instar larvae expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Pxn.PS have a reduced number of hemocytes recruited to a puncture wound site 6 hours after wounding compared to control larvae.

Primary hemocytes derived from larvae expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Cg.PA have enlarged endolysosomes.

(Velichkova et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^how-24B, mtm^RNAi.cVa.UAS has lethal phenotype, suppressible by Hsap\MTMR2^UAS.EGFP, Scer\GAL4^how-24B

(Ribeiro et al., 2011)

Scer\GAL4^Cg.PA, mtm^RNAi.cVa.UAS has lethal | pharate adult stage phenotype, suppressible by Hsap\MTMR2^UAS.EGFP, Scer\GAL4^Cg.PA

(Velichkova et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^He.PZ, mtm^RNAi.cVa.UAS has endolysosome phenotype, suppressible by 14-3-3ζ^GD16467, Scer\GAL4^He.PZ

(Pueyo et al., 2016)

Scer\GAL4^He.PZ, mtm^RNAi.cVa.UAS has hemocyte phenotype, suppressible by 14-3-3ζ^GD16467, Scer\GAL4^He.PZ

(Pueyo et al., 2016)

Scer\GAL4^Mef2.PR, mtm^RNAi.cVa.UAS has muscle cell of abdominal temporary eclosion muscle DA phenotype, suppressible by Pi3K68D^GD7348, Scer\GAL4^Mef2.PR

(Ribeiro et al., 2011)

Scer\GAL4^Cg.PA, mtm^RNAi.cVa.UAS has embryonic/larval hemocyte phenotype, suppressible by Pi3K68D^GD7348, Scer\GAL4^Cg.PA

(Velichkova et al., 2010)

Scer\GAL4^Cg.PA, mtm^RNAi.cVa.UAS has embryonic/larval hemocyte phenotype, suppressible by Pi3K68D^GD7348/Scer\GAL4^Pxn.PS

(Velichkova et al., 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^Mef2.PR, mtm^RNAi.cVa.UAS has muscle cell of abdominal temporary eclosion muscle DA phenotype, non-suppressible by Pi3K59F^KK107602, Scer\GAL4^Mef2.PR

(Ribeiro et al., 2011)

Scer\GAL4^Cg.PA, mtm^RNAi.cVa.UAS has embryonic/larval hemocyte phenotype, non-suppressible by Pi3K59F^KD.UAS/Scer\GAL4^Pxn.PS

(Velichkova et al., 2010)

Suppressor of

Statement

Reference

mtm^RNAi.cVa.UAS, Scer\GAL4^Cg.PA is a suppressor of embryonic/larval plasmatocyte phenotype of Sbf^UAS.mCherry, Scer\GAL4^Cg.PA

(Jean et al., 2012)

mtm^RNAi.cVa.UAS, Scer\GAL4^Cg.PA is a suppressor of embryonic/larval plasmatocyte phenotype of Rab21^UAS.GFP, Scer\GAL4^Cg.PA

(Jean et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of 14-3-3ζ^GD16467 rescues the increased endolysosomal compartment phenotype of hemocytes expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^He.PZ.

(Pueyo et al., 2016)

Expression of mtm^{dsRNA.cVa.Scer\UAS} suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing Sbf^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^Cg.PA. The macrophages display unextended F-actin fibers.

Expression of mtm^{dsRNA.cVa.Scer\UAS} suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing Rab21^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Cg.PA.

(Jean et al., 2012)

Co-expression of Pi3K68D^GD7348, but not of Pi3K59F^KK107602, rescues the detachment of the dorsal temporary internal oblique muscles which is seen in pharate adults expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Mef2.PR.

(Ribeiro et al., 2011)

Co-expression of Pi3K68D^GD7348 suppresses the enlarged endolysosome phenotype seen in primary hemocytes derived from larvae expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Cg.PA.

The lack of F-actin rich radial protrusions seen in primary hemocytes derived from larvae expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Pxn.PS is completely rescued by co-expression of Pi3K68D^GD7348.

The abnormal clumped distribution of sessile hemocytes seen in larvae expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Pxn.PS is rescued by co-expression of Pi3K68D^GD7348.

The lack of F-actin rich radial protrusions seen in primary hemocytes derived from larvae expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Pxn.PS is infrequently and only mildly modified by co-expression of Pi3K59F^KD.Scer\UAS.

(Velichkova et al., 2010)

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\MTMR2^{Scer\UAS.T:Avic\GFP-EGFP} rescues the lethality caused by expression of mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^how-24B.

(Ribeiro et al., 2011)

Co-expression of Hsap\MTMR2^{Scer\UAS.T:Avic\GFP-EGFP} rescues the lethality caused by expression of mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Cg.PA.

(Velichkova et al., 2010)

Complementation and Rescue Data

Rescued by

mtm^RNAi.cVa.UAS is rescued by Scer\GAL4^how-24B/mtm^C.UAS.EGFP

(Ribeiro et al., 2011)

mtm^RNAi.cVa.UAS is rescued by mtm^C.UAS.EGFP/Scer\GAL4^Cg.PA

(Velichkova et al., 2010)

Rescues

mtm^RNAi.cVa.UAS/Scer\GAL4^Cg.PA rescues mtm^UAS.mCherry

(Jean et al., 2012)

Comments

Expression of mtm^{dsRNA.cVa.Scer\UAS} rescues the extra protrusions seen in macrophages isolated from animals expressing mtm^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^Cg.PA.

(Jean et al., 2012)

Co-expression of mtm^{C.Scer\UAS.T:Avic\GFP-EGFP} rescues the lethality caused by expression of mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^how-24B.

(Ribeiro et al., 2011)

The lethality caused by expression of mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Cg.PA is rescued by co-expression of mtm^{C.Scer\UAS.T:Avic\GFP-EGFP}.

(Velichkova et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mtm^RNAi.cVa.UAS

mtm^{dsRNA.cVa.Scer\UAS}

mtm^{dsRNA.cVa.UAS}

Name Synonyms

Secondary FlyBase IDs

References (6)

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