FlyBase Allele Report: Dmel\Pi3K68D[GD7348]

General Information

Symbol

Dmel\Pi3K68D^GD7348

Species

D. melanogaster

Name

FlyBase ID

FBal0208069

Feature type

allele

Associated gene

Dmel\Pi3K68D

Associated Insertion(s)

Carried in Construct

P{GD7348}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD7348}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD7348

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

flightless, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

embryonic/larval plasmatocyte, with Scer\GAL4^Cg.PA

(Jean et al., 2012)

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

myofibril, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

sarcomere, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

Primary macrophages isolated from animals expressing two copies of Pi3K68D^GD7348 under the control of Scer\GAL4^Cg.PA lack F-actin rich protrusions.

(Jean et al., 2012)

Adults expressing Pi3K68D^GD7348 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in flightless flies.

Expression under the control of Scer\GAL4^Mef2.PR results in missing adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in degenerate myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in missing sarcomeres in the adult indirect flight muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7348

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Pi3K68D^GD7348, Scer\GAL4^Pxn.PS is a suppressor of embryonic/larval plasmatocyte phenotype of Sbf^GD12081, Scer\GAL4^Pxn.PS

(Jean et al., 2012)

Pi3K68D^GD7348, Scer\GAL4^Cg.PA is a suppressor of embryonic/larval plasmatocyte phenotype of Sbf^UAS.mCherry, Scer\GAL4^Cg.PA

(Jean et al., 2012)

Pi3K68D^GD7348, Scer\GAL4^Cg.PA is a suppressor of embryonic/larval plasmatocyte phenotype of Scer\GAL4^Cg.PA, mtm^UAS.mCherry

(Jean et al., 2012)

Pi3K68D^GD7348, Scer\GAL4^Mef2.PR is a suppressor of muscle cell of abdominal temporary eclosion muscle DA phenotype of Scer\GAL4^Mef2.PR, mtm^RNAi.cVa.UAS

(Ribeiro et al., 2011)

Pi3K68D^GD7348, Scer\GAL4^Cg.PA is a suppressor of embryonic/larval hemocyte phenotype of Scer\GAL4^Cg.PA, mtm^RNAi.cVa.UAS

(Velichkova et al., 2010)

Pi3K68D^GD7348/Scer\GAL4^Pxn.PS is a suppressor of embryonic/larval hemocyte phenotype of Scer\GAL4^Cg.PA, mtm^RNAi.cVa.UAS

(Velichkova et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Pi3K68D^GD7348 suppresses the loss of F-actin rich protrusions seen in macrophages isolated from animals expressing Sbf^GD12081 under the control of Scer\GAL4^Pxn.PS.

Expression of Pi3K68D^GD7348 suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing Sbf^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^Cg.PA.

Expression of Pi3K68D^GD7348 suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing mtm^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^Cg.PA.

(Jean et al., 2012)

Co-expression of Pi3K68D^GD7348 rescues the detachment of the dorsal temporary internal oblique muscles which is seen in pharate adults expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Mef2.PR.

(Ribeiro et al., 2011)

Co-expression of Pi3K68D^GD7348 suppresses the enlarged endolysosome phenotype seen in primary hemocytes derived from larvae expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Cg.PA.

The lack of F-actin rich radial protrusions seen in primary hemocytes derived from larvae expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Pxn.PS is completely rescued by co-expression of Pi3K68D^GD7348.

The abnormal clumped distribution of sessile hemocytes seen in larvae expressing mtm^{dsRNA.cVa.Scer\UAS} under the control of Scer\GAL4^Pxn.PS is rescued by co-expression of Pi3K68D^GD7348.

(Velichkova et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v16240