FlyBase Allele Report: Dmel\Sbf[GD12081]

General Information

Symbol

Dmel\Sbf^GD12081

Species

D. melanogaster

Name

FlyBase ID

FBal0208481

Feature type

allele

Associated gene

Dmel\Sbf

Associated Insertion(s)

Carried in Construct

P{GD12081}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12081}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12081

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of P-stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pharate adult stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

embryonic/larval plasmatocyte, with Scer\GAL4^Pxn.PS

(Jean et al., 2012)

Detailed Description

Statement

Reference

Primary macrophages isolated from animals expressing Sbf^GD12081 under the control of Scer\GAL4^Pxn.PS are able to spread flat as is seen in wild type, but unlike wild type they lack F-actin rich protrusions.

(Jean et al., 2012)

Adults expressing Sbf^GD12081 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12081

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Sbf^GD12081, Scer\GAL4^Pxn.PS has embryonic/larval plasmatocyte phenotype, suppressible by Pi3K68D^GD7348, Scer\GAL4^Pxn.PS

(Jean et al., 2012)

Sbf^GD12081, Scer\GAL4^Cg.PA has embryonic/larval plasmatocyte phenotype, suppressible by Rac1^V12.UAS, Scer\GAL4^Cg.PA

(Jean et al., 2012)

NOT suppressed by

Statement

Reference

Sbf^GD12081, Scer\GAL4^Cg.PA has embryonic/larval plasmatocyte phenotype, non-suppressible by mtm^UAS.mCherry, Scer\GAL4^Cg.PA

(Jean et al., 2012)

Sbf^GD12081, Scer\GAL4^Cg.PA has embryonic/larval plasmatocyte phenotype, non-suppressible by Rho1^V14.UAS, Scer\GAL4^Cg.PA

(Jean et al., 2012)

Enhancer of

Statement

Reference

Sbf^GD12081, Scer\GAL4^Cg.PA is an enhancer of embryonic/larval plasmatocyte phenotype of Rab21^GD9402, Scer\GAL4^Cg.PA

(Jean et al., 2012)

Suppressor of

Statement

Reference

Sbf^GD12081, Scer\GAL4^Cg.PA is a suppressor of embryonic/larval plasmatocyte phenotype of Scer\GAL4^Cg.PA, mtm^UAS.mCherry

(Jean et al., 2012)

Sbf^GD12081, Scer\GAL4^Cg.PA is a suppressor of embryonic/larval plasmatocyte phenotype of Rab21^UAS.GFP, Scer\GAL4^Cg.PA

(Jean et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Sbf^GD12081 suppresses the increase in F-actin rich protrusions seen in macrophages isolated from animals expressing mtm^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^Cg.PA. The macrophages completely lack protrusions, similar to what is seen when Sbf^GD12081 is expressed alone.

Expression of Pi3K68D^GD7348 suppresses the loss of F-actin rich protrusions seen in macrophages isolated from animals expressing Sbf^GD12081 under the control of Scer\GAL4^Pxn.PS.

Expression of Sbf^GD12081 enhances the loss of F-actin rich protrusions seen in macrophages isolated from animals expressing Rab21^GD9042 under the control of Scer\GAL4^Cg.PA.

Expression of Sbf^GD12081 suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing Rab21^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Cg.PA.

Expression of Rac1^V12.Scer\UAS suppresses the loss of F-actin rich protrusions seen in macrophages isolated from animals expressing Sbf^GD12081 under the control of Scer\GAL4^Cg.PA.

Expression of Rho1^V14.Scer\UAS fails to suppress the loss of F-actin rich protrusions seen in macrophages isolated from animals expressing Sbf^GD12081 under the control of Scer\GAL4^Cg.PA.

(Jean et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Cg.PA/Sbf^GD12081 rescues Sbf^UAS.mCherry

(Jean et al., 2012)

Comments

Expression of Sbf^GD12081 rescues the excess F-actin rich protrusions seen in macrophages isolated from animals expressing Sbf^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^Cg.PA.

(Jean et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v22317

w¹¹¹⁸; P{GD12081}v22317

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts