FB2024_03 , released June 25, 2024
Allele: Dmel\mtmUAS.mCherry
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General Information
Symbol
Dmel\mtmUAS.mCherry
Species
D. melanogaster
Name
FlyBase ID
FBal0258482
Feature type
allele
Associated gene
Dmel\mtm
Associated Insertion(s)
Carried in Construct
P{UAS-mCherry-mtm}
Also Known As
UAS-mCherry:mtm
Key Links
Transgenic product class
cDNA
(Velichkova et al., 2010)
wild_type
(Velichkova et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Velichkova et al., 2010)
wild_type
(Velichkova et al., 2010)
Mutagen
in vitro construct
(Velichkova et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-mCherry-mtm}
Cytology
Description

UAS regulatory sequences drive expression of a full-length mtm cDNA which is tagged with mCherry.

(Velichkova et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
mtm
Tagged with
mCherry
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Primary macrophages isolated from animals expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA have an excess number of F-actin rich protrusions compared to wild type.

      (Jean et al., 2012)

      Primary hemocytes dissected from wild-type larvae undergo remodelling from a round to a spread cell shape, having F-actin rich radial protrusions. Primary hemocytes derived larvae expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA have an excessive number of these protrusions compared to wild-type controls.

      Third instar larvae expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Pxn.PS have a normal number of bled hemocytes per larva and have a normal number of sessile hemocytes per segment, although the sessile hemocytes have an abnormally dispersed distribution compared to controls.

      Third instar larvae expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Pxn.PS have a reduced number of hemocytes recruited to a puncture wound site 6 hours after wounding compared to control larvae.

      (Velichkova et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4He.PZ rescues the increased endolysosomal compartment phenotype of hemo-A4/hemo-A4 mutant hemocytes.

      (Pueyo et al., 2016)

      Expression of SbfGD12081 suppresses the increase in F-actin rich protrusions seen in macrophages isolated from animals expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA. The macrophages completely lack protrusions, similar to what is seen when SbfGD12081 is expressed alone.

      Expression of Pi3K68DGD7348 suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA.

      Expression of Rab21T27N.Scer\UAS.T:Avic\GFP suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA.

      Expression of Rab21GD9042 suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA.

      Expression of Rab11KK108297 suppresses the excess F-actin rich protrusions seen in macrophages isolated from animals expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA.

      (Jean et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      mtmUAS.mCherry is rescued by mtmRNAi.cVa.UAS/Scer\GAL4Cg.PA

      (Jean et al., 2012)
      Comments

      Expression of mtmdsRNA.cVa.Scer\UAS rescues the extra protrusions seen in macrophages isolated from animals expressing mtmScer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4Cg.PA.

      (Jean et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      mtmScer\UAS.T:Disc\RFP-mCherry
      mtmUAS.mCherry
      Name Synonyms
      Secondary FlyBase IDs
        References (3)