FlyBase Allele Report: Dmel\Rheb[EP50.084]

General Information

Symbol

Dmel\Rheb^EP50.084

Species

D. melanogaster

Name

FlyBase ID

FBal0159901

Feature type

allele

Associated gene

Dmel\Rheb

Associated Insertion(s)

P{EP(loxP.UAS)y⁺.UAS}Rheb^EP50.084

Carried in Construct

Also Known As

UAS-Rheb^EP50.084

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{EP(loxP.UAS)y⁺.UAS}Rheb^EP50.084

Cytology

Description

Insertion of a P{EP(loxP.UAS)y⁺.UAS} element six nucleotides downstream of the 5' end of the first exon of transcript Rheb-RA.

(Stocker et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{EP(loxP.UAS)y⁺.UAS}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell size | somatic clone, with Scer\GAL4^Act5C.PP

(Juhász et al., 2008)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Stocker et al., 2003)

fat body | somatic clone, with Scer\GAL4^Act5C.PP

(Juhász et al., 2008)

ommatidium, with Scer\GAL4^GMR.PF

(Stocker et al., 2003)

Detailed Description

Statement

Reference

Expression of Rheb^EP50.084 under the control of Scer\GAL4^Act5C.PP increases cell size.

(Juhász et al., 2008)

Overexpression of Rheb^EP50.084 results in an increase in TR-avidin endocytic internalisation.

(Hennig et al., 2006)

Rheb^EP50.084; Scer\GAL4^GMR.PF animals exhibit eye overgrowth. Ommatidia in Scer\GAL4^GMR.PF/+; Rheb^EP50.084/+ flies are disorganized, and are on average 1.43 times the size of wild-type ommatidia.

(Stocker et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Rheb^EP50.084, Scer\GAL4^Act5C.PP has increased cell size | somatic clone phenotype, non-enhanceable by Pi3K59F^KD.UAS

(Juhász et al., 2008)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Rheb^EP50.084, Scer\GAL4^Act5C.PP has fat body | somatic clone phenotype, non-enhanceable by Pi3K59F^KD.UAS

(Juhász et al., 2008)

Suppressed by

Statement

Reference

Rheb^EP50.084, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by S6k^l-1/S6k^l-1

(Stocker et al., 2003)

Rheb^EP50.084, Scer\GAL4^GMR.PF, mTor^2L1/Tor[+] has ommatidium phenotype, suppressible | partially by S6k[+]/S6k^l-1

(Stocker et al., 2003)

Rheb^EP50.084, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible | partially by mTor^2L1/Tor[+]

(Stocker et al., 2003)

Rheb^EP50.084, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by gig^UAS.cTa/Tsc1^UAS.cTa, Scer\GAL4^GMR.PF

(Stocker et al., 2003)

NOT suppressed by

Statement

Reference

Rheb^EP50.084, Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by Akt¹/Akt³

(Stocker et al., 2003)

Rheb^EP50.084, Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by Pten^UAS.cGb, Scer\GAL4^GMR.PF

(Stocker et al., 2003)

NOT Suppressor of

Statement

Reference

Rheb^EP50.084, Scer\GAL4^Lsp2.PH is a non-suppressor of embryonic/larval fat body phenotype of Acn^UAS.Tag:MYC, Scer\GAL4^Lsp2.PH

(Haberman et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Rheb^EP50.084 does not suppress the ability of Acn^{Scer\UAS.T:Hsap\MYC} expressed under the control of Scer\GAL4^Lsp2.PH to induce autolysosome formation in the fat body in the absence of starvation.

(Haberman et al., 2010)

Expression of Rheb^EP50.084 under the control of Scer\GAL4^Act5C.PP increases cell size, and this increase is also observed in the presence of Pi3K59F^KD.Scer\UAS.

(Juhász et al., 2008)

The large size and disorganisation of ommatidia in Scer\GAL4^GMR.PF/+; Rheb^EP50.084/+ flies is unaffected by Pten^Scer\UAS.cGb. The large size and disorganisation of ommatidia in Scer\GAL4^GMR.PF/+; Rheb^EP50.084/+ flies is largely suppressed by Tsc1^Scer\UAS.cTa with gig^Scer\UAS.cTa (ommatidial are = 0.95 of wild-type size and are only slightly disorganised. The large size and disorganisation of ommatidia in Scer\GAL4^GMR.PF/+; Rheb^EP50.084/+ flies is unaffected by Akt1¹/Akt1³. The large size and disorganisation of ommatidia in Scer\GAL4^GMR.PF/+; Rheb^EP50.084/+ flies is suppressed by S6k^l-1/S6k^l-1. The large size and disorganisation of ommatidia in Scer\GAL4^GMR.PF/+; Rheb^EP50.084/+ flies is partially dominantly suppressed by Tor^2L1 (ommatidia are 1.27 times wild-type size, and slightly disorganised). This suppression is stronger when one the flies are also heterozygous for S6k^l-1 (ommatidia 1.16 times wild-type size, and not disorganised).

(Stocker et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

The "EP50.084" chromosome carries a closely linked lethal factor that cannot be reverted by mobilisation of the P{EP.Scer\UAS(loxP.Scer\UAS.y⁺).BN} element.

(Stocker et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rheb^EP50.084

(Juhász et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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