FB2024_03 , released June 25, 2024
Recombinant Construct: P{EP(loxP.UAS)y+.UAS}
Open Close
General Information
Symbol
P{EP(loxP.UAS)y+.UAS}
FlyBase ID
FBtp0019242
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
8 available
Molecular map
Description and Uses
Description

The P{EP(loxP.UAS)y+.UAS} element contains UAS sequences at both ends, with each copy of UAS being oriented outwards, so that when expression is driven by a GAL4 driver, transcription is directed outwards from both ends of the element, potentially driving overexpression of any nearby genomic loci in both directions. A y+t* marker is present in between the two UAS sequences. The 5' copy of UAS plus the y+t* marker are flanked by a pair of loxP sites, allowing them to be excised in the presence of P1cre recombinase.

(Sese et al., 2006)
Construct components
Component
Use(s)
Element type
Regulatory region(s)
UAS
Also carries
loxP
Marker(s)
y+t*
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

construct_comment: Double headed enhancer piracy construct in which the 5\' Scer\UAS is flanked by \'loxP\' sites. Constructed by Konrad Basler and Denis Nellen.

(Stocker et al., 2003)

Double headed enhancer piracy construct in which the 5\' Scer\UAS is flanked by \'loxP\' sites. Constructed by Konrad Basler and Denis Nellen.

(Stocker et al., 2003)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{EP(loxP.UAS)y+.UAS}
(FlyBase, 1996-)
P{EP.Scer\UAS(loxP.Scer\UAS.y+).BN}
(Stocker et al., 2003, FlyBase, 1996-)
Secondary FlyBase IDs
    References (5)