FlyBase Allele Report: Dmel\mTor[2L1]

General Information

Symbol

Dmel\mTor^2L1

Species

D. melanogaster

Name

FlyBase ID

FBal0120583

Feature type

allele

Associated gene

Dmel\mTor

Associated Insertion(s)

Carried in Construct

Also Known As

TOR^2L1, dTOR^2L1

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Zhang et al., 2006)

Mutagen

ethyl methanesulfonate

(Oldham et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: P2303L. This mutation is within the highly conserved area of the kinase domain.

(Oldham et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:13,012,397..13,012,397 [+]

Nucleotide change:

C13012397T

Amino acid change:

P2293L | Tor-PA; P2294L | Tor-PB

Reported amino acid change:

P2303L

Comment:

Position of mutation on reference sequence inferred by FlyBase curator. Difference between annotated and reported sites of amino acid substitution due to authors reported CDS being 10aa longer.

(Oldham et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval somatic muscle cell

(Tixier et al., 2013)

embryonic myoblast

(Tixier et al., 2013)

head | somatic clone

(Oldham et al., 2000)

larval fat cell | somatic clone - Minute background | third instar larval stage

(Devilliers et al., 2021)

larval salivary gland & nucleus (with mTor^k17004)

(Oldham et al., 2000)

segment border muscle cell

(Tixier et al., 2013)

Detailed Description

Statement

Reference

Tor^2L1/Tor^2L1 fat body clones in a Minute background, induced during embryogenesis and analyzed at early third instar larval stage, are small and bear smaller cells than controls.

(Devilliers et al., 2021)

Tor^2L1/Tor^2L19 larvae are fat (increased TAG levels) and small in size.

(Tiebe et al., 2015)

The number of nuclei in the segmental border muscle is slightly reduced compared to wild type in mutant embryos.

(Tixier et al., 2013)

Mutant embryos have thinner muscles than normal and unfused myoblasts are present.

(Tixier et al., 2013)

Cells in Tor^2L1 mutant clones in the eye are 0.26 times the size of their heterozygous neighbours.

(Gao et al., 2002)

Tissue specific induction of Tor^2L1 mutant clones in the head produces flies with reduced head size relative to the body. Tor^2L1/Tor^k17004 mutants have wing discs that are approximately wild-type in size. The size of the nuclei in the endoreplicating salivary glands are smaller than wild-type.

(Oldham et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mTor^2L1 has decreased cell size | third instar larval stage | somatic clone - Minute background phenotype, suppressible by Pten¹¹⁷/Pten¹¹⁷

(Devilliers et al., 2021)

mTor^2L1/mTor^2L19 has decreased body size | larval stage phenotype, suppressible | partially by REPTOR^KO

(Tiebe et al., 2015)

Tsc1^Q87X, mTor^2L1/Tor[+] has lethal phenotype, suppressible | partially by S6k[+]/S6k^l-1

(Radimerski et al., 2002)

NOT suppressed by

Statement

Reference

mTor^2L1 has decreased cell size | third instar larval stage | somatic clone - Minute background phenotype, non-suppressible by Rheb^{EP50.084-loxP}/Scer\GAL4^Tub.PU

(Devilliers et al., 2021)

mTor^2L1 has decreased cell size | somatic clone phenotype, non-suppressible by Tsc1²⁹/Tsc1²⁹

(Gao et al., 2002)

Suppressor of

Statement

Reference

mTor^2L1/Tor[+] is a suppressor | partially of lethal | larval stage phenotype of gig¹⁹³/gig¹⁰⁹, mTor^+t9.4

(Zhang et al., 2006)

mTor^2L1/Tor[+] is a suppressor | partially of lethal | larval stage phenotype of gig¹⁹³/gig¹⁰⁹

(Zhang et al., 2006)

mTor^2L1/Tor[+] is a suppressor of increased cell size | somatic clone phenotype of Tsc1²⁹

(Gao et al., 2002)

mTor^2L1/Tor[+] is a suppressor of lethal | recessive | larval stage phenotype of Tsc1²⁹

(Gao et al., 2002)

mTor^2L1/Tor[+] is a suppressor of lethal phenotype of Tsc1^Q87X

(Radimerski et al., 2002)

mTor^2L1/Tor[+] is a suppressor | partially of lethal phenotype of S6k[+]/S6k^l-1, Tsc1^Q87X

(Radimerski et al., 2002)

Other

Statement

Reference

Pten¹¹⁷, mTor^2L1 has decreased cell number | third instar larval stage | somatic clone - Minute background phenotype

(Devilliers et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

mTor^2L1 has larval fat cell | third instar larval stage | somatic clone - Minute background phenotype, suppressible by Pten¹¹⁷/Pten¹¹⁷

(Devilliers et al., 2021)

Rheb^EP50.084, Scer\GAL4^GMR.PF, mTor^2L1/Tor[+] has ommatidium phenotype, suppressible | partially by S6k[+]/S6k^l-1

(Stocker et al., 2003)

NOT suppressed by

Statement

Reference

mTor^2L1 has larval fat cell | third instar larval stage | somatic clone - Minute background phenotype, non-suppressible by Rheb^{EP50.084-loxP}/Scer\GAL4^Tub.PU

(Devilliers et al., 2021)

Suppressor of

Statement

Reference

mTor^2L1/Tor[+] is a suppressor | partially of ommatidium phenotype of Rheb^EP50.084, Scer\GAL4^GMR.PF

(Stocker et al., 2003)

Other

Statement

Reference

Pten¹¹⁷, mTor^2L1 has larval fat cell | third instar larval stage | somatic clone - Minute background phenotype

(Devilliers et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

The small cell size in Tor^2L1/Tor^2L1 third instar larval fat body clones (in a Minute background) is suppressed by clonal Pten¹¹⁷, even leading to larger cells than in controls.

(Devilliers et al., 2021)

REPTOR^KO significantly rescues both the metabolic and size phenotypes of Tor^2L1/Tor^2L19 larvae.

(Tiebe et al., 2015)

Around 60% of Tor^2L1/+; gig¹⁰⁹/gig¹⁹³ flies survive to adulthood, while no gig¹⁰⁹/gig¹⁹³ survive to the pupal stage. The Tor^+t9.4 transgene reduces the amount of Tor^2L1/+; gig¹⁰⁹/gig¹⁹³ flies that survive to the pupal stage from around 85% to around 65%.

(Zhang et al., 2006)

The large size and disorganisation of ommatidia in Scer\GAL4^GMR.PF/+; Rheb^EP50.084/+ flies is partially dominantly suppressed by Tor^2L1 (ommatidia are 1.27 times wild-type size, and slightly disorganised).

(Stocker et al., 2003)

The first instar larval lethality due to Tsc1²⁹ is partially suppressed by heterozygosity for Tor^2L1 : 31% (n = 193) of Tor^2L1/+ Tsc1²⁹/Tsc1²⁹ animals survive to the pupal stage. The increased cell size seen in somatic clones of Tsc1²⁹ in the eye is partially suppressed in a Tor^2L1 heterozygous background: the resulting clone cells are 1.3X wild-type size, compared to 1.9X for Tsc1²⁹ somatic clone cells in a wild-type background. Cells in Tor^2L1; Tsc1²⁹ double mutant somatic clones are approximately 0.25 times the size of their heterozygous neighbours - i.e.- have little or no size difference with Tor^2L1 somatic clone cells.

(Gao et al., 2002)

The second instar lethality of homozygous Tsc1^Q87X is rescued to adulthood (18.5%) or pupal stages (82.5%) by heterozygosity for Tor^2L1. The second instar lethality of homozygous Tsc1^Q87X is rescued to adulthood (62%) or pupal stages (93%) by heterozygosity for both S6k^l-1 and Tor^2L1. The rescued animals are slightly larger than wild-type flies, with overall patterning appearing normal. The rescued females are semi-fertile when crossed to wild-type males, whereas the rescued males are fully fertile when crossed to wild-type females.

(Radimerski et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

mTor^W1251R

(Zhang et al., 2006)

mTor^A948V

(Zhang et al., 2006)

mTor^R97C

(Zhang et al., 2006)