FlyBase Allele Report: Dmel\Rheb[EP50.084-loxP]

General Information

Symbol

Dmel\Rheb^{EP50.084-loxP}

Species

D. melanogaster

Name

FlyBase ID

FBal0159902

Feature type

allele

Associated gene

Dmel\Rheb

Associated Insertion(s)

P{EP(-loxP)y⁺.UAS}Rheb^{EP50.084-loxP}

Carried in Construct

Also Known As

UAS-Rheb

Key Links

Allele class

Mutagen

cre recombinase

Nature of the Allele

Allele class

Mutagen

cre recombinase

(Stocker et al., 2003)

Progenitor genotype

P{EP(loxP.UAS)y⁺.UAS}Rheb^EP50.084

(Stocker et al., 2003)

Associated Insertion(s)

P{EP(-loxP)y⁺.UAS}Rheb^{EP50.084-loxP}

Cytology

Description

The loxP cassette in the P{EP(loxP.UAS)y⁺.UAS} insertion has been excised, converting the inserted element to a single-headed P{EP(-loxP)y⁺.UAS} element capable of driving Rheb expression. in the presence of a GAL4 driver.

(Stocker et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{EP(-loxP)y⁺.UAS}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of disease of metabolism

CEA

(Devilliers et al., 2021)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Fat body clones expressing Rheb^{EP50.084-loxP} under the control of Scer\GAL4^Tub.PU, induced during embryogenesis and analyzed at the early third instar larval stage, bear larger cells than controls.

(Devilliers et al., 2021)

Clones expressing Rheb^{EP50.084-loxP} under the control of Scer\GAL4^Act5C.PU in the third larval instar wing disc and fat body are larger than control clones.

(Ibar et al., 2013)

Ovarian follicle cells overexpressing Rheb^{EP50.084-loxP} (under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}) lack lysotracker staining even under starvation conditions, indicating that activation of IIS/TOR signaling is sufficient to suppress starvation-induced autophagy.

(Barth et al., 2011)

Cells in the fat body that are expressing Rheb^{EP50.084-loxP} show an increase in relative cell area compared to controls.

(Kim et al., 2008)

Overexpression of Rheb^{EP50.084-loxP} under the control of Scer\GAL4⁷⁹⁶ induces a significant increase in the synaptic domain.

(Martin-Pena et al., 2006)

Expression of Rheb^{EP50.084-loxP} under the control of Scer\GAL4^GMR.PF results in a bulging eye phenotype.

(Reiling and Hafen, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU has increased cell size | third instar larval stage phenotype, enhanceable by Pten¹¹⁷/Pten¹¹⁷

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP} has increased cell size phenotype, enhanceable by RagC-D^EY11726/RagC-D^EY11726

(Kim et al., 2008)

Suppressed by

Statement

Reference

Pten¹¹⁷, Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU has increased cell size | third instar larval stage phenotype, suppressible | partially by Df(2L)FASN^Δ24-23/Df(2L)FASN^Δ24-23

(Devilliers et al., 2021)

NOT suppressed by

Statement

Reference

Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU has increased cell size | third instar larval stage phenotype, non-suppressible by Scer\GAL4^da.PU/Pfk^GD1508

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU has increased cell size | third instar larval stage phenotype, non-suppressible by Scer\GAL4^da.PU/Ldh^GD6887

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU has increased cell size | third instar larval stage phenotype, non-suppressible by Scer\GAL4^da.PU/Pyk^GD16178

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU has increased cell size | third instar larval stage phenotype, non-suppressible by Scer\GAL4^da.PU/Pdha1^GD12103

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU has increased cell size | third instar larval stage phenotype, non-suppressible by Df(2L)FASN^Δ24-23/Df(2L)FASN^Δ24-23

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}, Scer\GAL4^GMR.PF has increased body size phenotype, non-suppressible by chrb^EP1035/scyl^EP22.1, Scer\GAL4^GMR.PF

(Reiling and Hafen, 2004)

Enhancer of

Statement

Reference

Rheb^{EP50.084-loxP}/Scer\GAL4^Tub.PU is an enhancer of increased cell size | third instar larval stage phenotype of Pten¹¹⁷

(Devilliers et al., 2021)

Suppressor of

Statement

Reference

Rheb^{EP50.084-loxP}, Scer\GAL4^Cg.PA is a suppressor of decreased cell size phenotype of Atg1^GS10797, Scer\GAL4^Cg.PA

(Scott et al., 2007)

NOT Suppressor of

Statement

Reference

Rheb^{EP50.084-loxP}/Scer\GAL4^Tub.PU is a non-suppressor of decreased cell size | third instar larval stage | somatic clone - Minute background phenotype of mTor^ΔP

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}/Scer\GAL4^Tub.PU is a non-suppressor of decreased cell size | third instar larval stage | somatic clone - Minute background phenotype of mTor^2L19

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}/Scer\GAL4^Tub.PU is a non-suppressor of decreased cell size | third instar larval stage | somatic clone - Minute background phenotype of mTor^2L1

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}, Scer\GAL4^salm.EPv is a non-suppressor of visible phenotype of Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462

(Ibar et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU has larval fat cell | third instar larval stage | somatic clone phenotype, enhanceable by Pten¹¹⁷/Pten¹¹⁷

(Devilliers et al., 2021)

Suppressed by

Statement

Reference

Pten¹¹⁷, Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU has larval fat cell | somatic clone | third instar larval stage phenotype, suppressible | partially by Df(2L)FASN^Δ24-23/Df(2L)FASN^Δ24-23

(Devilliers et al., 2021)

NOT suppressed by

Statement

Reference

Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU has larval fat cell | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4^da.PU/Pfk^GD1508

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU has larval fat cell | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4^da.PU/Pyk^GD16178

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU has larval fat cell | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4^da.PU/Ldh^GD6887

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU has larval fat cell | somatic clone | third instar larval stage phenotype, non-suppressible by Scer\GAL4^da.PU/Pdha1^GD12103

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU has larval fat cell | somatic clone | third instar larval stage phenotype, non-suppressible by Df(2L)FASN^Δ24-23/Df(2L)FASN^Δ24-23

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by chrb^EP1035/scyl^EP22.1, Scer\GAL4^GMR.PF

(Reiling and Hafen, 2004)

Enhancer of

Statement

Reference

Rheb^{EP50.084-loxP}/Scer\GAL4^Tub.PU is an enhancer of larval fat cell | third instar larval stage | somatic clone phenotype of Pten¹¹⁷

(Devilliers et al., 2021)

Suppressor of

Statement

Reference

Rheb^{EP50.084-loxP}, Scer\GAL4^Cg.PA is a suppressor of fat body phenotype of Atg1^GS10797, Scer\GAL4^Cg.PA

(Scott et al., 2007)

NOT Suppressor of

Statement

Reference

Rheb^{EP50.084-loxP}/Scer\GAL4^Tub.PU is a non-suppressor of larval fat cell | third instar larval stage | somatic clone - Minute background phenotype of mTor^ΔP

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}/Scer\GAL4^Tub.PU is a non-suppressor of larval fat cell | third instar larval stage | somatic clone - Minute background phenotype of mTor^2L19

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}/Scer\GAL4^Tub.PU is a non-suppressor of larval fat cell | third instar larval stage | somatic clone - Minute background phenotype of mTor^2L1

(Devilliers et al., 2021)

Rheb^{EP50.084-loxP}, Scer\GAL4^salm.EPv is a non-suppressor of 1st posterior cell phenotype of Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462

(Ibar et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in the distance between wing veins 3 and 4 that is seen in animals expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^salm.EPv is not suppressed if they are also co-expressing Rheb^{EP50.084-loxP}.

Clones expressing Prpk^{dsRNA.Scer\UAS.462} and also co-expressing Rheb^{EP50.084-loxP} under the control of Scer\GAL4^Act5C.PU in the third larval instar wing disc and fat body are small and apoptotic, showing the same phenotype as clones expressing Prpk^{dsRNA.Scer\UAS.462} alone under the control of Scer\GAL4^Act5C.PU.

(Ibar et al., 2013)

The increase in cell area relative to control cells that is seen in cells in the fat body that are expressing Rheb^{EP50.084-loxP} is enhanced if the animals are also homozygous for RagC^EY11726.

(Kim et al., 2008)

The variable reduction in cell size and induction of autophagy in the fat body that is seen in animals expressing Atg1^GS10797 under the control of Scer\GAL4^Cg.PA is suppressed if the animals are also co-expressing Rheb^{EP50.084-loxP}.

(Scott et al., 2007)

The bulging eye phenotype caused by expression of Rheb^{EP50.084-loxP} under the control of Scer\GAL4^GMR.PF is not suppressed by simultaneous coexpression of both scyl^EP22.1 and chrb^EP1035.

(Reiling and Hafen, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

EPRheb

(Reiling and Hafen, 2004)

Rheb^{EP50.084-loxP}

Rheb^OE

(Kim et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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