FlyBase Allele Report: Dmel\Pdha1[GD12103]

General Information

Symbol

Dmel\Pdha1^GD12103

Species

D. melanogaster

Name

FlyBase ID

FBal0209834

Feature type

allele

Associated gene

Dmel\Pdha1

Associated Insertion(s)

Carried in Construct

P{GD12103}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12103}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12103

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of disease of metabolism

CEA

(Devilliers et al., 2021)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

abnormal wound healing | larval stage, with Scer\GAL4^repo

(Li et al., 2020)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

lethal - all die before end of larval stage, with Scer\GAL4^da.PU

(Devilliers et al., 2021)

short lived, with Scer\GAL4^da.PU

(Devilliers et al., 2021)

short lived, with Scer\GAL4^elav-C155

(Besson et al., 2015)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

larval multidendritic class IV neuron, with Scer\GAL4^repo

(Li et al., 2020)

Detailed Description

Statement

Reference

Flies expressing l(1)G0334^GD12103 in neurons under the control of Scer\GAL4^elav-C155 show a dramatic reduction in lifespan compared with wild-type.

(Besson et al., 2015)

Adults expressing l(1)G0334^GD12103 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in adult/pharate lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12103

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pdha1^GD12103, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by Pfk^UAS.cTa, Scer\GAL4^elav-C155

(Besson et al., 2015)

Pdha1^GD12103, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by G6pd^UAS.cLa, Scer\GAL4^elav-C155

(Besson et al., 2015)

Pdha1^GD12103, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by Hsap\SLC2A3^UAS.cBa, Scer\GAL4^elav-C155

(Besson et al., 2015)

NOT Enhancer of

Statement

Reference

Pdha1^GD12103, Scer\GAL4^repo is a non-enhancer of abnormal wound healing | larval stage phenotype of L2HGDH^GD5322, Scer\GAL4^repo

(Li et al., 2020)

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Pdha1^GD12103 is a suppressor | partially of increased cell size | third instar larval stage phenotype of Pten¹¹⁷

(Devilliers et al., 2021)

Pdha1^GD12103/Scer\GAL4^ppk.PU is a suppressor of abnormal wound healing | larval stage phenotype of Myc^QUAS.cPa, Ncra\QF^repo

(Li et al., 2020)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Pdha1^GD12103 is a non-suppressor of increased cell size | third instar larval stage phenotype of Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU

(Devilliers et al., 2021)

Pdha1^GD12103, Scer\GAL4^repo is a non-suppressor of abnormal wound healing | larval stage phenotype of L2HGDH^GD5322, Scer\GAL4^repo

(Li et al., 2020)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Pdha1^GD12103, Scer\GAL4^repo is a non-enhancer of larval multidendritic class IV neuron phenotype of L2HGDH^GD5322, Scer\GAL4^repo

(Li et al., 2020)

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Pdha1^GD12103 is a suppressor | partially of larval fat cell | somatic clone | third instar larval stage phenotype of Pten¹¹⁷

(Devilliers et al., 2021)

Pdha1^GD12103/Scer\GAL4^ppk.PU is a suppressor of larval multidendritic class IV neuron phenotype of Myc^QUAS.cPa, Ncra\QF^repo

(Li et al., 2020)

Pdha1^GD12103/Scer\GAL4^elav.PLu is a suppressor of lipid droplet | cell non-autonomous | adult stage phenotype of ND-42^ninaE.GD6220

(Liu et al., 2017)

Pdha1^GD12103, Scer\GAL4^nSyb.PS is a suppressor of adult optic lobe glial cell | cell non-autonomous phenotype of SREBP^UAS.cKa, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Pdha1^GD12103, Scer\GAL4^nSyb.PS is a suppressor of lipid droplet | cell non-autonomous | adult stage phenotype of SREBP^UAS.cKa, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Pdha1^GD12103, Scer\GAL4^nSyb.PS is a suppressor of adult optic lobe glial cell | cell non-autonomous phenotype of Scer\GAL4^nSyb.PS, bsk^UAS.cBa

(Liu et al., 2017)

Pdha1^GD12103, Scer\GAL4^nSyb.PS is a suppressor of lipid droplet | cell non-autonomous | adult stage phenotype of Scer\GAL4^nSyb.PS, bsk^UAS.cBa

(Liu et al., 2017)

Pdha1^GD12103/Scer\GAL4^elav.PLu is a suppressor of adult optic lobe glial cell | cell non-autonomous phenotype of ND-42^ninaE.GD6220

(Liu et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Pdha1^GD12103 is a non-suppressor of larval fat cell | somatic clone | third instar larval stage phenotype of Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU

(Devilliers et al., 2021)

Pdha1^GD12103, Scer\GAL4^repo is a non-suppressor of larval multidendritic class IV neuron phenotype of L2HGDH^GD5322, Scer\GAL4^repo

(Li et al., 2020)

Scer\GAL4^54C/Pdha1^GD12103 is a non-suppressor of adult optic lobe glial cell | cell non-autonomous phenotype of ND-42^ninaE.GD6220

(Liu et al., 2017)

Scer\GAL4^54C/Pdha1^GD12103 is a non-suppressor of lipid droplet | cell non-autonomous | adult stage phenotype of ND-42^ninaE.GD6220

(Liu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The accumulation of lipid droplets in glial cells in the vicinity of adult photoreceptor neurons expressing ND-42^ninaE.GD6220 is not suppressed by Scer\GAL4^54C-driven (glial) expression of Pdha^GD12103 while expression under the control of the neuronal Scer\GAL4^elav.PLu driver efficiently rescues the phenotype.

The accumulation of lipid droplets in the retinal glia of adult flies expressing either SREBP^UAS.cKa or bsk^Scer\UAS.cBa under the control of Scer\GAL4^nSyb.PS is significantly suppressed by co-expression of Pdha^GD12103.

(Liu et al., 2017)

Co-expression of Pfk^Scer\UAS.cTa significantly improves the survival of flies expressing Scer\GAL4^elav-C155>l(1)G0334^GD12103.

Co-expression of Zw^Scer\UAS.cLa significantly improves the survival of flies expressing Scer\GAL4^elav-C155>l(1)G0334^GD12103.

(Besson et al., 2015)

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\SLC2A3^Scer\UAS.cBa significantly suppresses the life-expectancy defects resulting from the expression of Scer\GAL4^elav-C155>l(1)G0334^GD12103.

(Besson et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v40410

w¹¹¹⁸; P{GD12103}v40410

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12103

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Pdha1^GD12103

Pdha^GD12103

l(1)G0334^GD12103

Name Synonyms

Secondary FlyBase IDs

References (11)

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