FlyBase Allele Report: Dmel\awd[RNAi.UAS]

General Information

Symbol

Dmel\awd^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0240499

Feature type

allele

Associated gene

Dmel\awd

Associated Insertion(s)

Carried in Construct

P{UAS-awd.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Woolworth et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Woolworth et al., 2009)

Mutagen

in vitro construct

(Woolworth et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-awd.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of tail-to-tail tandem copies of the first 150 nucleotides of the awd open reading frame.

(Woolworth et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

awd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

follicle cell, with Scer\GAL4^55B

(Woolworth et al., 2009)

Detailed Description

Statement

Reference

Epithelial defects are seen in the egg chambers of females expressing awd^{dsRNA.Scer\UAS} under the control of Scer\GAL4^55B; 30% show flattening of epithelial cells, 11% show gaps in the epithelium, 27% show piling up or loose cells, and 7% show invasion of the germ cell complex by the follicle cells.

(Woolworth et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^55B, awd^RNAi.UAS has follicle cell phenotype, non-enhanceable by Rab5^{S43N.UASp.YFP}, Scer\GAL4^55B

(Woolworth et al., 2009)

Suppressed by

Statement

Reference

Scer\GAL4^55B, awd^RNAi.UAS has follicle cell phenotype, suppressible | partially by Rab5^{Q88L.UASp.YFP}, Scer\GAL4^55B

(Woolworth et al., 2009)

Scer\GAL4^55B, awd^RNAi.UAS has follicle cell phenotype, suppressible | partially by Rab5^UASp.YFP, Scer\GAL4^55B

(Woolworth et al., 2009)

NOT suppressed by

Statement

Reference

Scer\GAL4^55B, awd^RNAi.UAS has follicle cell phenotype, non-suppressible by Rab5^{S43N.UASp.YFP}, Scer\GAL4^55B

(Woolworth et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The follicle cell epithelial defects seen in females expressing awd^{dsRNA.Scer\UAS} under the control of Scer\GAL4^55B are rescued by co-expression of either Rab5^{Scer\UAS.P\T.T:Avic\GFP-YFP} or Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP}, but are not rescued or exacerbated by co-expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP}.

(Woolworth et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

awd^RNAi.UAS

awd^{dsRNA.Scer\UAS}

awd^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close