FlyBase Allele Report: Dmel\vap[2]

General Information

Symbol

Dmel\vap²

Species

D. melanogaster

Name

FlyBase ID

FBal0151357

Feature type

allele

Associated gene

Dmel\vap

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Botella et al., 2003)

Mutagen

Progenitor genotype

Cytology

Description

Deletion of 239 base pairs starting from position 999 (by the numbering of cDNA AJ012609). This deletion removes a splice donor site at 1099 bp. The resulting predicted protein, from a transcript using a splice donor site at 907 bp, is lacking amino acids 206-269.

(Botella et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Rowshanravan et al., 2014)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Rowshanravan et al., 2014)

abnormal starvation stress response | adult stage

(Bourouis et al., 2019)

short lived | calorie restriction conditional

(Bourouis et al., 2019)

short lived | recessive

(Botella et al., 2003)

viable

(Bourouis et al., 2019)

Phenotype Manifest In

adult | nutrition conditional

(Bourouis et al., 2019)

adult brain

(Rowshanravan et al., 2014, Botella et al., 2003)

Detailed Description

Statement

Reference

vap² homozygous adults show significantly decreased body-mass when raised in standard diet, but not in poor diet, as compared to controls. These adults are hypersensitive to acute food deprivation, but not if fed 15% sucrose only.

(Bourouis et al., 2019)

The maximum lifespan of vap² homozygous flies is 25 days, compared to 90 days for wild-type controls. Vacuolisation is apparent in brains vap² homozygous flies by 15 days of age.

(Botella et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

vap² has abnormal starvation stress response | adult stage phenotype, suppressible by spri^6G1/spri^6G1

(Bourouis et al., 2019)

vap² has short lived | calorie restriction conditional phenotype, suppressible by spri^6G1/spri^6G1

(Bourouis et al., 2019)

vap² has abnormal starvation stress response | adult stage phenotype, suppressible by spri^6G1/spri[+]

(Bourouis et al., 2019)

vap² has short lived | calorie restriction conditional phenotype, suppressible by spri^6G1/spri[+]

(Bourouis et al., 2019)

vap² has abnormal neuroanatomy phenotype, suppressible by spri^6G1/spri[+]

(Rowshanravan et al., 2014)

vap² has abnormal neuroanatomy phenotype, suppressible by Rab5[+]/Rab5⁴

(Rowshanravan et al., 2014)

vap² has abnormal neuroanatomy phenotype, suppressible by Rab5²/Rab5[+]

(Rowshanravan et al., 2014)

Other

Statement

Reference

spri^6G1, vap² has abnormal starvation stress response | adult stage phenotype

(Bourouis et al., 2019)

spri^6G1, vap² has short lived | calorie restriction conditional phenotype

(Bourouis et al., 2019)

Ras85D^V12.C40.UAS, Scer\GAL4^Appl.G1a, vap² has lethal phenotype

(Botella et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

vap² has adult optic lobe | adult stage phenotype, enhanceable by Egfr^WT.hs

(Botella et al., 2003)

vap² has adult optic lobe | adult stage phenotype, enhanceable by Ras85D^UAS.cKa/Scer\GAL4^Appl.G1a

(Botella et al., 2003)

vap² has adult optic lobe | adult stage phenotype, enhanceable by rho^hs.PSt

(Botella et al., 2003)

vap² has adult optic lobe | adult stage phenotype, enhanceable by sty[+]/sty^S73

(Botella et al., 2003)

Suppressed by

Statement

Reference

vap² has adult brain phenotype, suppressible by spri^6G1

(Rowshanravan et al., 2014)

vap² has adult brain phenotype, suppressible by Rab5[+]/Rab5⁴

(Rowshanravan et al., 2014)

vap² has adult brain phenotype, suppressible by Rab5²/Rab5[+]

(Rowshanravan et al., 2014)

vap² has adult optic lobe | adult stage phenotype, suppressible by Egfr^f3/Egfr[+]

(Botella et al., 2003)

vap² has adult optic lobe | adult stage phenotype, suppressible by drk^k02401/drk[+]

(Botella et al., 2003)

vap² has adult optic lobe | adult stage phenotype, suppressible by Egfr[+]/Egfr^tsla

(Botella et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

vap²/vap², spri^6G1/spri^6G1 and vap²/+, spri^6G1/spri^6G1 double mutants show the same increased resistance to acute food deprivation as spri^6G1/spri^6G1 single mutants, instead of the hypersensitivity observed in vap²/vap² single mutants.

(Bourouis et al., 2019)

A homozygous spri^6G1 mutant background significantly suppresses the vap² neurodegenerative phenotype. Weaker suppression is seen in a spri^6G1 heterozygous background.

A heterozygous Rab5⁴ mutant background suppresses the vap² neurodegenerative phenotype.

A heterozygous Rab5² mutant background significantly suppresses the vap² neurodegenerative phenotype.

(Rowshanravan et al., 2014)

The neurodegenerative phenotype of 18 day old vap² homozygous flies is dominantly enhanced by sty^S73 and by rho^hs.PSt or Egfr^WT.hs under heat shock conditions or Ras85D^Scer\UAS.cKa; Scer\GAL4^Appl.G1a. (Assayed by the percentage of vacuolisation in the optic lobes in 18 day old adults). The neurodegenerative phenotype of 18 day old vap² homozygous flies is largely suppressed by Egfr^f3/+ or drk^k02401/+, or by reduction of Egfr levels in adulthood using the temperature sensitive allele Egfr^tsla/+. (Assayed by the percentage of vacuolisation in the optic lobes in 18 day old adults). The genetic combination: vap²; Ras85D^{V12.C40.Scer\UAS}; Scer\GAL4^Appl.G1a is lethal.

(Botella et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

vap² is rescued by Scer\GAL4^elav.PU/vap^{SH23mut2.UAS.Tag:MYC}

(Rowshanravan et al., 2014)

vap² is rescued by Scer\GAL4^elav.PU/vap^UAS.Tag:MYC

(Rowshanravan et al., 2014)

Not rescued by

vap² is not rescued by Scer\GAL4^elav.PU/vap^{SH2mut32.UAS.Tag:MYC}

(Rowshanravan et al., 2014)

Comments

Expression of vap^{SH2mut32.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PU fails to rescue the neurodegeneration phenotype seen in vap² mutant flies.

Expression of vap^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PU rescues the neurodegeneration phenotype seen in vap² mutant flies.

Expression of vap^{SH23mut2.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PU rescues the neurodegeneration phenotype seen in vap² mutant flies.

(Rowshanravan et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

vap²

(Bourouis et al., 2019, Rowshanravan et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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