FlyBase Allele Report: Dmel\Egfr[f3]

General Information

Symbol

Dmel\Egfr^f3

Species

D. melanogaster

Name

FlyBase ID

FBal0003531

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

Also Known As

top^IP02, top^1P02, flb^IP02, flb^1P02, flb^1PO2

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Raz et al., 1991, Clifford and Schupbach, 1989, Schejter and Shilo, 1989)

loss of function allele

(Thackeray et al., 1998)

Mutagen

ethyl methanesulfonate

(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: W1075term.

(Clifford and Schupbach, 1994)

Nucleotide substitution: G3731A.

(Clifford and Schupbach, 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:21,558,173..21,558,173 [+]

Nucleotide change:

G21558173A

Reported nucleotide change:

G3731A

Amino acid change:

W1075term | Egfr-PA; W1124term | Egfr-PB

Reported amino acid change:

W1075term

Comment:

TGG to TGA

(Clifford and Schupbach, 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | recessive

(Radke et al., 2001, Clifford and Schupbach, 1994, Clifford and Schupbach, 1989, Schejter and Shilo, 1989)

some die during embryonic stage | recessive

(Radke et al., 2001, Clifford and Schupbach, 1994, Clifford and Schupbach, 1989, Schejter and Shilo, 1989)

Phenotype Manifest In

embryonic/first instar larval cuticle

(Radke et al., 2001)

embryonic/first instar larval cuticle | ventral

(Simcox, 1997)

follicle cell (with Egfr^t1)

(Smith et al., 2002)

ventral denticle belt

(Radke et al., 2001)

wing vein L4 (with Egfr^t2)

(Freeman, 1996)

Detailed Description

Statement

Reference

Homozygous embryos have a "faint little ball" phenotype; they lack denticle setae and anterior, posterior and ventral cuticular structures.

(Radke et al., 2001)

Egfr^f3/Df(2R)Egfr3 embryos exhibit no ventral cuticle and the anterior has a large hole. No Egfr^f3/Df(2R)Egfr3 mutant discs could be in vivo cultured.

(Simcox, 1997)

Egfr^f3/Egfr^t2 wings have a gap in L4 wing vein.

(Freeman, 1996)

Transheterozygous females with Egfr^t1 display egg chambers with gaps in the follicular epithelium that uncover the nurse cells. Eggs derived from these females display weak or intermediate ventralised phenotypes.

(Goode et al., 1996)

Enhances the female sterility and adult morphological defects of Egfr^t1. Rarely survives as transheterozygote with the semi-viable Egfr^top-CA allele.

(Clifford and Schupbach, 1994)

Homozygotes and hemizygotes display a severe 'flb' phenotype. Embryos produced from heteroallelic combination with Egfr^t1 have a severe ventralised phenotype, reduction in size of their dorsal appendage.

(Clifford and Schupbach, 1989)

severe allele

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Egfr^f3 is an enhancer of visible phenotype of Scer\GAL4^en-e16E, arm^UAS.cWa

(Greaves et al., 1999)

NOT Enhancer of

Statement

Reference

Egfr^f3 is a non-enhancer of visible phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Greaves et al., 1999)

Suppressor of

Statement

Reference

Egfr^f3 is a suppressor | partially of visible phenotype of Scer\GAL4^sca-109-68, ato^UAS.cJa

(White and Jarman, 2000)

NOT Suppressor of

Statement

Reference

Egfr^f3 is a non-suppressor of visible phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Greaves et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

Egfr^f3/Egfr^t1 has follicle cell phenotype, enhanceable by da[+]/da²

(Smith et al., 2002)

Suppressed by

Statement

Reference

Egfr^f3 has phenotype, suppressible by eRF1[+]/eRF1^F2

(Chao et al., 2003)

Egfr^f3/Egfr^t2 has wing vein L4 phenotype, suppressible by sev::tor^13D.hs.sev

(Freeman, 1996)

Enhancer of

Statement

Reference

Egfr^f3 is an enhancer of photoreceptor cell R7 phenotype of Scer\GAL4^sca-109-68, ato^UAS.cJa, sev¹⁴

(White and Jarman, 2000)

Egfr^f3 is an enhancer of wing phenotype of Scer\GAL4^en-e16E, arm^UAS.cWa

(Greaves et al., 1999)

Egfr^f3 is an enhancer of phenotype of svp^hs.ro1

(Kramer et al., 1995)

NOT Enhancer of

Statement

Reference

Egfr^f3 is a non-enhancer of wing phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Greaves et al., 1999)

Egfr^f3 is a non-enhancer of eye phenotype of N^spl-1

(Nagel and Preiss, 1999)

Suppressor of

Statement

Reference

Egfr^f3/Egfr[+] is a suppressor of adult optic lobe | adult stage phenotype of vap²

(Botella et al., 2003)

Egfr^f3 is a suppressor | partially of eye phenotype of Scer\GAL4^sca-109-68, ato^UAS.cJa

(White and Jarman, 2000)

Egfr^f3/Egfr[+] is a suppressor of photoreceptor cell R7 | ectopic phenotype of sl¹

(Thackeray et al., 1998)

Egfr^f3/Egfr[+] is a suppressor of photoreceptor cell R7 | ectopic phenotype of sl²

(Thackeray et al., 1998)

Egfr^f3 is a suppressor of phenotype of spi^s.sev

(Freeman, 1996)

Egfr^f3 is a suppressor of phenotype of svp^2.sev

(Kramer et al., 1995)

NOT Suppressor of

Statement

Reference

Egfr^f3 is a non-suppressor of wing phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Greaves et al., 1999)

Other

Statement

Reference

Egfr^f3, Scer\GAL4^sca-109-68, ato^UAS.cJa, sev¹⁴ has photoreceptor cell R7 phenotype

(White and Jarman, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Suppresses the recruitment defects in the eye caused by expression of ato^Scer\UAS.cJa under the control of Scer\GAL4^sca-109-68, although abnormal pattern formation still occurs. Many R7 cells differentiate in sev¹⁴ flies expressing ato^Scer\UAS.cJa under the control of Scer\GAL4^sca-109-68 (in contrast to sev¹⁴ flies which lack R7 photoreceptors). This rescue of R7 formation by ato^Scer\UAS.cJa expressed under the control of Scer\GAL4^sca-109-68 is strongly suppressed if the flies are also carrying Egfr^f3.

(White and Jarman, 2000)

Has no effect on the eye phenotype of N^spl-1.

(Nagel and Preiss, 1999)

Eye supernumerary R7 cell phenotype of sl¹ and sl² is almost completely suppressed by reducing the dosage of Egfr.

(Thackeray et al., 1998)

Egfr^f3/Egfr^t2 vein gap can be rescued by one copy of sev::tor^13D.hs.sev. Ectopic expression also causes extra vein phenotype, notably around the distal end of L2 and broadening or deltas at the distal tips of most of the veins.

(Freeman, 1996)

No effect on the faf eye phenotype.

(Huang and Fischer-Vize, 1996)

Suppression of the rough eye phenotype (formation of extra R7 cells) caused by P{sev-svp2} and enhancement of the P{ro-svp1} rough eye phenotype (loss of one or more outer photoreceptors from many of the ommatidia).

(Kramer et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

34043

cn¹ Egfr^f3 bw¹ speck¹/CyO

Notes on Origin

Discoverer

Selected as: Embryonic lethal.

Comments

Mutation of Egfr that coordinately affects all gene activities, a class I lesion. The allelic series for class I lesions: Egfr^t2 = Egfr^t1 < Egfr^top-EC20 < Egfr^f7 = Egfr^f1 = Egfr^flb-2E07 < Egfr^top-EE39 = Egfr^top-ED26 = Egfr^f5 < Egfr^f9 = Egfr^f10 = Egfr^f2 = Egfr^top-EE42 = Egfr^f11 = Egfr^f24 = Egfr^f3 = Df(2R)Egfr3.

(Clifford and Schupbach, 1989)

Class I allele.

(Clifford and Schupbach, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

DER^1P02

(Huang and Fischer-Vize, 1996)

DER^IP02

(Simcox, 1997)

EGFR^F3

(Crossman et al., 2018)

Egfr^1P02

(Kramer et al., 1995)

Egfr^f3

Egfr^top1PO2

(Botella et al., 2003)

IP02

(Shamloula et al., 2002)

flb

flb^1P02

(Freeman, 1996, Schweitzer et al., 1995, Schejter and Shilo, 1989)

flb^1PO2

(Thackeray et al., 1998, Raz et al., 1991)

flb^IP02

(Greaves et al., 1999, Nagel and Preiss, 1999, Raz et al., 1991)

flb^IP

(Tearle and Nusslein-Volhard, 1987)

top^1P02

(Radke et al., 2001, Clifford and Schupbach, 1994, Clifford and Schupbach, 1989)

top^IP02

(Chang et al., 2008, Smith et al., 2002, Goode et al., 1996)

Name Synonyms

faint little ball

Secondary FlyBase IDs

References (23)

Report Sections

Open Close