FlyBase Allele Report: Dmel\Egfr[t2]

General Information

Symbol

Dmel\Egfr^t2

Species

D. melanogaster

Name

FlyBase ID

FBal0003573

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

Also Known As

top^CJ, egfr^CJ

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C406T. Amino acid replacement: S58F.

(Clifford and Schupbach, 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:21,554,848..21,554,848 [+]

Nucleotide change:

C21554848T

Reported nucleotide change:

C406T

Amino acid change:

S58F | Egfr-PA; S107F | Egfr-PB

Reported amino acid change:

S58F

(Clifford and Schupbach, 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Price et al., 1989)

female sterile | recessive

(Clifford and Schupbach, 1989)

lethal - all die during embryonic stage | maternal effect

(Roth and Schupbach, 1994)

some die during embryonic stage | maternal effect

(Roth and Schupbach, 1994)

viable

(Baker and Rubin, 1992)

Phenotype Manifest In

dorsal appendage | maternal effect

(Roth and Schupbach, 1994)

ventral furrow | maternal effect

(Roth and Schupbach, 1994)

wing vein L4 (with Egfr^f3)

(Freeman, 1996)

Detailed Description

Statement

Reference

Dsor1 mutant alleles do not suppress the eggshell dorso-ventral polarity defect of the Egfr^t2 maternal mutation.

(Lim et al., 1997)

Egfr^f3/Egfr^t2 wings have a gap in L4 wing vein.

(Freeman, 1996)

Transheterozygous females with Egfr^f2 display egg chambers with gaps in the follicular epithelium that uncover the nurse cells. Eggs derived from these females display weak or intermediate ventralised phenotypes.

(Goode et al., 1996)

Expression of phl^F22.hs in the anterior follicle cells of heterozygotes with Egfr^t1 causes dorsalisation of the egg chamber.

(Brand and Perrimon, 1994)

Homozygous adult viable.

(Clifford and Schupbach, 1994)

Weak ventralization of embryos. Filzkorper are normal. Only one dorsal appendage forms. 51% of laid eggs contain embryos. Mesoderm is wider than usual and cannot be enveloped by remaining epidermis. Two ventral furrows form.

(Roth and Schupbach, 1994)

Does not suppress the "Ellipse" phenotype. Hemizygotes die during pupariation, and have abnormally small dorsal imaginal discs.

(Baker and Rubin, 1992)

Viable as a homozygote and hemizygote. Adults exhibit milder eye defects than would be expected from the severity of the wing and haltere defects.

(Clifford and Schupbach, 1989)

maternal-effect lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Egfr^f3/Egfr^t2 has wing vein L4 phenotype, suppressible by sev::tor^13D.hs.sev

(Freeman, 1996)

Other

Statement

Reference

Egfr^t2, hup¹ has follicle cell phenotype

(Rotoli et al., 1998)

Egfr^t2, hup¹ has nurse cell phenotype

(Rotoli et al., 1998)

Egfr^t2, hup¹ has egg chamber phenotype

(Rotoli et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

16% of Egfr^t2 hup¹ double homozygous egg chambers are dicephalic, and an additional 12.5% of double homozygous egg chambers (either dicephalic or with the oocyte normally localised) show degenerated nurse cells. 10% of double homozygous egg chambers have multiple layers of follicle cells at the posterior end. In some cases, these follicle cells try to migrate centripetally at the posterior, splitting the cytoplasm of the oocyte.

(Rotoli et al., 1998)

Egfr^f3/Egfr^t2 vein gap can be rescued by one copy of sev::tor^13D.hs.sev. Ectopic expression also causes extra vein phenotype, notably around the distal end of L2 and broadening or deltas at the distal tips of most of the veins.

(Freeman, 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Mutation of Egfr that coordinately affects all gene activities, a class I lesion. The allelic series for class I lesions: Egfr^t2 = Egfr^t1 < Egfr^top-EC20 < Egfr^f7 = Egfr^f1 = Egfr^flb-2E07 < Egfr^top-EE39 = Egfr^top-ED26 = Egfr^f5 < Egfr^f9 = Egfr^f10 = Egfr^f2 = Egfr^top-EE42 = Egfr^f11 = Egfr^f24 = Egfr^f3 = Df(2R)Egfr3.

(Clifford and Schupbach, 1989)

Class I allele.

(Clifford and Schupbach, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Egf-r^CJ

(Sturtevant et al., 1993)

Egfr^CJ

(Neuman-Silberberg and Schupbach, 1996)

Egfr^t2

(Kim et al., 2023)

egfr^CJ

(Ghiglione et al., 1999)

top^CJ

(Grammont et al., 2000, Rotoli et al., 1998, Sapir et al., 1998, Lim et al., 1997, Goode et al., 1996, Brand and Perrimon, 1994, Clifford and Schupbach, 1994, Roth and Schupbach, 1994, Baker and Rubin, 1992, Clifford and Schupbach, 1989, Price et al., 1989)

top^cj

(Freeman, 1996)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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