Homozygous and hup1/T(2;4)DTD38 females lay eggs with variable phenotypes and both dorsalised and ventralised eggs are seen. 100% of these eggs have abnormal dorsal appendages, of which 83% have shorter dorsal appendages than normal, 14% have fused dorsal appendages and 3% lack dorsal appendages. The oocyte is displaced from its posterior location in 15.5% of hup1/T(2;4)DTD38 egg chambers, and in 10% of stage S14 egg chambers, a micropyle is present at each pole. Dicephalic egg chambers or eggs with two micropyles are not seen in homozygous hup1 ovaries, although oocytes with an anterior localisation are occasionally seen. Females bearing homozygous germline clones can produce progeny.
Oogenesis proceeds normally in most egg chambers until they reach stage 11, when the distribution of follicle cell nuclei is very irregular with more nuclei in the anterior, and no nuclei in other areas of the egg chamber. Nuclei are also irregular in shape. The few eggs laid by homozygous mothers are large with shorter and thicker dorsal appendages than wild type.
Nearly completely penetrant upheld wing. Homozygotes viable and semi-sterile in both sexes. Lethal in combination with deficiency. Mortality of zygotes produced by hup mothers increased by the presence of a paternally inherited Y chromosome but not by the quantity of paternally derived X heterochromatin. Maternal effect at 25oC more severe than at 19oC. Fertility also temperature-sensitive; spermatids of sterile males show micronuclei and occasional double basal bodies and axonemes.
Egfrt2, hup1 has follicle cell phenotype
Egfrt2, hup1 has nurse cell phenotype
Egfrt2, hup1 has egg chamber phenotype
16% of Egfrt2 hup1 double homozygous egg chambers are dicephalic, and an additional 12.5% of double homozygous egg chambers (either dicephalic or with the oocyte normally localised) show degenerated nurse cells. 10% of double homozygous egg chambers have multiple layers of follicle cells at the posterior end. In some cases, these follicle cells try to migrate centripetally at the posterior, splitting the cytoplasm of the oocyte.
