FlyBase Allele Report: Dmel\Vap33[Δ20]

General Information

Symbol

Dmel\Vap33^Δ20

Species

D. melanogaster

Name

FlyBase ID

FBal0141328

Feature type

allele

Associated gene

Dmel\Vap33

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Pennetta et al., 2002)

Mutagen

P-element activity

(Pennetta et al., 2002)

Progenitor genotype

Vap33⁴⁷

(Pennetta et al., 2002)

Cytology

Description

Imprecise excision of P{lArB} of Vap-33-1⁴⁷ removing 2.0kb of flanking DNA, including Vap-33-1 coding region.

(Pennetta et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis type 8

CEA

(Mao et al., 2019)

model of autosomal dominant adult-onset proximal spinal muscular atrophy

CEA

(Mao et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of amyotrophic lateral sclerosis type 8

is ameliorated by Rab5²

(Mao et al., 2019)

is ameliorated by Rab7^Gal4-KO

(Mao et al., 2019)

model of autosomal dominant adult-onset proximal spinal muscular atrophy

is ameliorated by Rab5²

(Mao et al., 2019)

is ameliorated by Rab7^Gal4-KO

(Mao et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Pennetta et al., 2002)

lethal

(Chai et al., 2008)

lethal | recessive

(Ratnaparkhi et al., 2008)

lethal - all die before end of larval stage | recessive

(Pennetta et al., 2002)

some die during larval stage | recessive

(Pennetta et al., 2002)

Phenotype Manifest In

adult mushroom body alpha-lobe | pupal stage

(Tsuda et al., 2008)

bouton

(Pennetta et al., 2002)

Detailed Description

Statement

Reference

Vap-33-1^Δ20 mutants lack mushroom body α lobes in late pupae and adult brains.

(Tsuda et al., 2008)

Lethality acts in the 2nd/early 3rd instar. Number of boutons on embryonic somatic muscles is 50% wild type, though muscle surface area is normal. The size of the boutons is abnormally large, such that synaptic surface area for all boutons on a muscle is similar to that of wild type.

(Pennetta et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Vap33^Δ20 has lethal phenotype, suppressible by Hsap\VAPB^P56S.UAS/Scer\GAL4^C164

(Chai et al., 2008)

Vap33^Δ20 has lethal phenotype, suppressible by Hsap\VAPB^P56S.UAS/Scer\GAL4^Toll-6-D42

(Chai et al., 2008)

Phenotype Manifest In

Other

Statement

Reference

Eph^unspecified, Vap33^Δ20 has adult mushroom body alpha-lobe | pupal stage phenotype

(Tsuda et al., 2008)

Eph^unspecified, Vap33^Δ20 has adult mushroom body alpha-lobe | adult stage phenotype

(Tsuda et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Vap-33-1^Δ20 Eph^unspecified double mutants lack mushroom body α lobes in late pupae and adult brains.

(Tsuda et al., 2008)

Xenogenetic Interactions

Statement

Reference

Vap-33-1^Δ20 mutant lethality is rescued through expression of Hsap\VAPB^{P56S.Scer\UAS} under the control of either Scer\GAL4^C164 or Scer\GAL4^D42. However, the number of boutons is not significantly different in flies expressing Hsap\VAPB^{P56S.Scer\UAS} compared to controls. Moreover, no significant difference in EJPs or quantal sizes is observed in flies expressing Hsap\VAPB^{P56S.Scer\UAS}.

(Chai et al., 2008)

Complementation and Rescue Data

Rescued by

Vap33^Δ20 is rescued by Vap33^UAS.cPa/Scer\GAL4^C164

(Pennetta et al., 2002)

Vap33^Δ20 is rescued by Vap33^hs.PP

(Pennetta et al., 2002)

Partially rescued by

Vap33^Δ20 is partially rescued by Vap33^UAS.cRa/Scer\GAL4^elav.PLu

(Ratnaparkhi et al., 2008)

Vap33^Δ20 is partially rescued by Vap33^P58S.cRa.UAS/Scer\GAL4^elav.PLu

(Ratnaparkhi et al., 2008)